CAJ | 학술논문

目的：研究驻极体对3T3细胞内游离Ca2+浓度变化的影响，探讨驻极体诱导3T3细胞凋亡与游离Ca2+浓度变化之间的关系。方法：选用-300 V和-500 V 驻极体作用3T3细胞48 h，即时依次加入CaCl2、A23187、EDTA，通过流式细胞术检测细胞内Ca2+浓度的变化和3T3细胞的凋亡量。结果：(1)负极性驻极体作用3T3细胞后，3T3细胞出现10%的凋亡量；(2)驻极体作用3T3细胞以后，细胞质游离Ca2+浓度升高，Ca2+浓度的升高不是由于细胞内钙池的释放，而是由于细胞外Ca2+内流所引起。结论：驻极体诱导3T3细胞凋亡的机制可能是细胞外Ca2+内流引起的。
목적：연구주겁체대3T3세포내유리Ca2+농도변화적영향，탐토주겁체유도3T3세포조망여유리Ca2+농도변화지간적관계。방법：선용-300 V화-500 V 주겁체작용3T3세포48 h，즉시의차가입CaCl2、A23187、EDTA，통과류식세포술검측세포내Ca2+농도적변화화3T3세포적조망량。결과：(1)부겁성주겁체작용3T3세포후，3T3세포출현10%적조망량；(2)주겁체작용3T3세포이후，세포질유리Ca2+농도승고，Ca2+농도적승고불시유우세포내개지적석방，이시유우세포외Ca2+내류소인기。결론：주겁체유도3T3세포조망적궤제가능시세포외Ca2+내류인기적。
Objective: To study the changes of intracellular free Ca2+ levels in 3T3 cells induced by electrets and discuss the relationship between electrets induced apoptosis of 3T3 cells and the changes of free Ca2+ levels. Methods: After 48 h action of -300 V and -500 V electrets in 3T3 cells, CaCl2， A23187， EDTA were added in proper order to 3T3 cells. The levels of intracellular Ca2+ and the cell number of apoptosis were detected with flow cytometry. Results：（1）After the action of electrets, 10% of 3T3 cells appeared apoptosis. (2) After the action of electrets in 3T3 cells, the free Ca2+ levels in cellular plasm were increased. The increasement of Ca2+ levels was caused by the Ca2+ inflow from outside cell, rather than the release of calcium pool inside cell. Conclusion：The electret can induce Ca2+ outside 3T3 cell to flow into cells, thereby induce cell apoptosis.