CAJ | 학술논문

实验旨在研究金属硫蛋白(MT)在大鼠成骨细胞对高锌和锌缺乏的耐受中的作用。采用反义MT寡核苷酸造成大鼠成骨细胞MT低表达，采用银／血红素饱和法测定细胞MT含量，以受损细胞所释放乳酸脱氢酶的活性表示细胞毒性。结果显示：反义MT寡核苷酸显著降低成骨细胞对高剂量锌以及锌缺乏的耐受性，使细胞毒性增加，表明MT在维系成骨细胞锌稳态以及对抗高剂量锌和锌缺乏的毒性中具有重要调节作用。
실험지재연구금속류단백(MT)재대서성골세포대고자화자결핍적내수중적작용。채용반의MT과핵감산조성대서성골세포MT저표체，채용은／혈홍소포화법측정세포MT함량，이수손세포소석방유산탈경매적활성표시세포독성。결과현시：반의MT과핵감산현저강저성골세포대고제량자이급자결핍적내수성，사세포독성증가，표명MT재유계성골세포자은태이급대항고제량자화자결핍적독성중구유중요조절작용。
This study is expected to investigate the role ofmetallothionein(MT) in the resistance of osteoblasts to high or low zinc conditions. Antisense MT oligonucleotide was used to reduce the content of MT in osteoblast. Celluar MT content was determined by silver／ hemolysate saturation method, and the cytotoxicity was measured by the activity of LDH releasing from damaged cells. The results showed that antisense MT oligonucleotide markedly decreased the resistance of osteoblast to zinc excess and zinc deficiency. It suggested that MT played an important role in the modulation and maintenance of intracellular zinc homeostasis of osteoblasts.