中国介入心脏病学杂志
中國介入心髒病學雜誌
중국개입심장병학잡지
CHINESE JOURNAL OF INTERVENTIONAL CARDIOLOGY
2001年
2期
101-103
,共3页
姜宁%高炜%王日胜%朱建健%郭艳红%王建%朱国英%沈冬%陈光慧%汤健
薑寧%高煒%王日勝%硃建健%郭豔紅%王建%硃國英%瀋鼕%陳光慧%湯健
강저%고위%왕일성%주건건%곽염홍%왕건%주국영%침동%진광혜%탕건
促血管形成素-1%基因治疗%血管闭塞病
促血管形成素-1%基因治療%血管閉塞病
촉혈관형성소-1%기인치료%혈관폐새병
目的观察肌肉电转促血管形成素-1（angiopoietin-1，Ang-1）基因对大鼠下肢血管闭塞病的治疗效果。方法制作大鼠下肢血管闭塞病模型，通过电转Ang-1进行基因治疗，以RT-PCR及免疫组化方法观察Ang-1基因的表达，应用微血管计数、血管造影技术及相关临床指标观察Ang-1基因导入大鼠体内的生物学效应。结果（1）肌肉电转pcD2Ang-1基因可在大鼠局部转基因肌肉组织高效表达Ang-1；（2）转pcD2Ang-1基因组大鼠下肢肌肉组织新生血管及侧支循环数目明显多于同期空载质粒对照组。结论转Ang-1基因可促进大鼠局部组织新生血管形成和侧支循环建立，恢复闭塞部位血供，从而有效治疗血管闭塞病。
目的觀察肌肉電轉促血管形成素-1（angiopoietin-1，Ang-1）基因對大鼠下肢血管閉塞病的治療效果。方法製作大鼠下肢血管閉塞病模型，通過電轉Ang-1進行基因治療，以RT-PCR及免疫組化方法觀察Ang-1基因的錶達，應用微血管計數、血管造影技術及相關臨床指標觀察Ang-1基因導入大鼠體內的生物學效應。結果（1）肌肉電轉pcD2Ang-1基因可在大鼠跼部轉基因肌肉組織高效錶達Ang-1；（2）轉pcD2Ang-1基因組大鼠下肢肌肉組織新生血管及側支循環數目明顯多于同期空載質粒對照組。結論轉Ang-1基因可促進大鼠跼部組織新生血管形成和側支循環建立，恢複閉塞部位血供，從而有效治療血管閉塞病。
목적관찰기육전전촉혈관형성소-1（angiopoietin-1，Ang-1）기인대대서하지혈관폐새병적치료효과。방법제작대서하지혈관폐새병모형，통과전전Ang-1진행기인치료，이RT-PCR급면역조화방법관찰Ang-1기인적표체，응용미혈관계수、혈관조영기술급상관림상지표관찰Ang-1기인도입대서체내적생물학효응。결과（1）기육전전pcD2Ang-1기인가재대서국부전기인기육조직고효표체Ang-1；（2）전pcD2Ang-1기인조대서하지기육조직신생혈관급측지순배수목명현다우동기공재질립대조조。결론전Ang-1기인가촉진대서국부조직신생혈관형성화측지순배건립，회복폐새부위혈공，종이유효치료혈관폐새병。
Objective We tested the hypothesis that gene transfer of plasmid DNA encoding angiopoietin-1 (Ang-1) could modulate collateral vessel development in a rat model of hindlimb ischemia. Methods PcD2 and pcD2 / Ang-1 were electroporated intramuscularly into the rat ischemic hindlimb. Couateral vessel development and limb perfusion were assessed by capillary density, capillary / muscle fiber ratio, angiograghy and clinical observation. Results (1) The expression of Ang-1 gene was comfirmed RT-PCR and immunohistochemistry; (2) Collateral vessels developed more with pcD2/Ang-1 than those with pcD2. Conclusion Intramuscular electroporation of Ang-1 gene produces anatomic and physiological evidence of enhanced collateral vessels formation. Ang-1 may modulate neovascularization in adult animals and thus represents a feasible therapeutic strategy for patients with tissue ischemia.
