中国中药杂志
中國中藥雜誌
중국중약잡지
CHINA JOURNAL OF CHINESE MATERIA MEDICA
2007年
3期
249-252
,共4页
袁拯忠%朱陵群%庞鹤%单泽松%王硕仁%高永红%牛福玲
袁拯忠%硃陵群%龐鶴%單澤鬆%王碩仁%高永紅%牛福玲
원증충%주릉군%방학%단택송%왕석인%고영홍%우복령
黄连解毒汤%脑微血管内皮细胞%缺氧/复氧%栀子苷%黄芩苷%小檗碱
黃連解毒湯%腦微血管內皮細胞%缺氧/複氧%梔子苷%黃芩苷%小檗堿
황련해독탕%뇌미혈관내피세포%결양/복양%치자감%황금감%소벽감
目的:研究栀子苷、黄芩苷和小檗碱对大鼠脑微血管内皮细胞的保护作用.方法:建立离体大鼠脑微血管内皮细胞缺氧/复氧损伤模型,用1.024,0.512,0.256,0.128,0.064,0.032,0.016,0.008 μmol·mL-1栀子苷,0.224,0.112,0.056,0.028,0.014,0.007,0.003 μnol·mL-1黄芩苷和0.192,0.096,0.048,0.024,0.012,0.006,0.003 μmol·mL-1小檗碱分别作用于正常组和模型组细胞.细胞活性用MTT比色法检测.结果:0.128,0.064,0.032 μmol·mL-1栀子苷,0.028,0.014,0.007 μmol·mL-1黄芩苷和0.024,0.012,0.006 μmol·mL-1小檗碱能够比较理想的保护缺氧4 h复氧12 h损伤的大鼠脑微血管内皮细胞.结论:适当浓度的栀子苷、黄芩苷和小檗碱等黄连解毒汤主要成分可以保护脑微血管内皮细胞.
目的:研究梔子苷、黃芩苷和小檗堿對大鼠腦微血管內皮細胞的保護作用.方法:建立離體大鼠腦微血管內皮細胞缺氧/複氧損傷模型,用1.024,0.512,0.256,0.128,0.064,0.032,0.016,0.008 μmol·mL-1梔子苷,0.224,0.112,0.056,0.028,0.014,0.007,0.003 μnol·mL-1黃芩苷和0.192,0.096,0.048,0.024,0.012,0.006,0.003 μmol·mL-1小檗堿分彆作用于正常組和模型組細胞.細胞活性用MTT比色法檢測.結果:0.128,0.064,0.032 μmol·mL-1梔子苷,0.028,0.014,0.007 μmol·mL-1黃芩苷和0.024,0.012,0.006 μmol·mL-1小檗堿能夠比較理想的保護缺氧4 h複氧12 h損傷的大鼠腦微血管內皮細胞.結論:適噹濃度的梔子苷、黃芩苷和小檗堿等黃連解毒湯主要成分可以保護腦微血管內皮細胞.
목적:연구치자감、황금감화소벽감대대서뇌미혈관내피세포적보호작용.방법:건립리체대서뇌미혈관내피세포결양/복양손상모형,용1.024,0.512,0.256,0.128,0.064,0.032,0.016,0.008 μmol·mL-1치자감,0.224,0.112,0.056,0.028,0.014,0.007,0.003 μnol·mL-1황금감화0.192,0.096,0.048,0.024,0.012,0.006,0.003 μmol·mL-1소벽감분별작용우정상조화모형조세포.세포활성용MTT비색법검측.결과:0.128,0.064,0.032 μmol·mL-1치자감,0.028,0.014,0.007 μmol·mL-1황금감화0.024,0.012,0.006 μmol·mL-1소벽감능구비교이상적보호결양4 h복양12 h손상적대서뇌미혈관내피세포.결론:괄당농도적치자감、황금감화소벽감등황련해독탕주요성분가이보호뇌미혈관내피세포.
