中国组织工程研究与临床康复
中國組織工程研究與臨床康複
중국조직공정연구여림상강복
JOURNAL OF CLINICAL REHABILITATIVE TISSUE ENGINEERING RESEARCH
2008年
16期
3193-3197
,共5页
牙周组织再生%骨髓间充质干细胞%细胞密度
牙週組織再生%骨髓間充質榦細胞%細胞密度
아주조직재생%골수간충질간세포%세포밀도
背景:目前临床上II度根分叉病变区牙周组织的再生仍是一项难题.目的:比较不同密度自体骨髓间充质干细胞修复II度牙根分叉病变区牙周组织缺损的能力.设计:随机对照观察实验.单位:福建医科大学附属口腔医院实验室和福州总医院动物实验科.材料:实验于2005-07/2006-09在解放军南京军区福州总医院动物实验科和福建医科大学附属口腔医院实验室完成.6只1岁半雄性Beagle犬由解放军南京军区福州总医院动物实验科提供,实验过程中对动物处置符合动物伦理学标准.实验中选用Bio-Gide胶原膜和BME-10X胶原膜.方法:在犬的下颌第2,3前磨牙和第1磨牙颊侧根分叉处制备慢性II度牙根分叉病变模型.从6只18月龄的Beagle犬抽取自体骨髓间充质干细胞.应用5×108 L-1,5×109 L-1,5×1010 L-1的骨髓间充质干细胞复合BME-10X胶原膜移植治疗牙根分叉病变,表面覆盖Bi0-Gide胶原膜,单纯Bi0-Gide胶原膜组为对照组.采用OLYPUS IX 71显微照相系统和OLYSIA BioAutoCell软件计算新生牙骨质长度的百分比和新生牙槽骨面积的百分比.主要观察指标:苏木精-伊红染色观察并测量牙周组织再生(新生牙骨质长度和新生牙槽骨面积的百分比)情况.结果:对照组的新生牙骨质长度和新生牙槽骨面积的百分比分别为:(51.5±5.6)%和(27.1±7.7)%;5×108 L-1骨髓间充质干细胞组为:(84.8±8.9)%和(30.6±7.7)%; 5×109 L-1骨髓间充质干细胞组为:(91.8±5.2)%和(68.3±11.4)%;5×1010 L-1骨髓间充质干细胞组为:(88.8±7.2)%和(78.5±12.7)%.细胞组的新生牙骨质量与对照组相比,差异有显著性意义(P < 0.01),但各细胞组间相互比较差异无显著性意义;5×109 L-1组和5×1010 L-1组的新生牙槽骨量与 5×108 L-1组和对照组相比差异有显著性 (P < 0.05),但前两组间和后两组间比较则差异无显著性意义.结论:骨髓间充质干细胞移植能促进牙根分叉病变区的牙周组织再生,其作用与细胞的接种密度有关.
揹景:目前臨床上II度根分扠病變區牙週組織的再生仍是一項難題.目的:比較不同密度自體骨髓間充質榦細胞脩複II度牙根分扠病變區牙週組織缺損的能力.設計:隨機對照觀察實驗.單位:福建醫科大學附屬口腔醫院實驗室和福州總醫院動物實驗科.材料:實驗于2005-07/2006-09在解放軍南京軍區福州總醫院動物實驗科和福建醫科大學附屬口腔醫院實驗室完成.6隻1歲半雄性Beagle犬由解放軍南京軍區福州總醫院動物實驗科提供,實驗過程中對動物處置符閤動物倫理學標準.實驗中選用Bio-Gide膠原膜和BME-10X膠原膜.方法:在犬的下頜第2,3前磨牙和第1磨牙頰側根分扠處製備慢性II度牙根分扠病變模型.從6隻18月齡的Beagle犬抽取自體骨髓間充質榦細胞.應用5×108 L-1,5×109 L-1,5×1010 L-1的骨髓間充質榦細胞複閤BME-10X膠原膜移植治療牙根分扠病變,錶麵覆蓋Bi0-Gide膠原膜,單純Bi0-Gide膠原膜組為對照組.採用OLYPUS IX 71顯微照相繫統和OLYSIA BioAutoCell軟件計算新生牙骨質長度的百分比和新生牙槽骨麵積的百分比.主要觀察指標:囌木精-伊紅染色觀察併測量牙週組織再生(新生牙骨質長度和新生牙槽骨麵積的百分比)情況.結果:對照組的新生牙骨質長度和新生牙槽骨麵積的百分比分彆為:(51.5±5.6)%和(27.1±7.7)%;5×108 L-1骨髓間充質榦細胞組為:(84.8±8.9)%和(30.6±7.7)%; 5×109 L-1骨髓間充質榦細胞組為:(91.8±5.2)%和(68.3±11.4)%;5×1010 L-1骨髓間充質榦細胞組為:(88.8±7.2)%和(78.5±12.7)%.細胞組的新生牙骨質量與對照組相比,差異有顯著性意義(P < 0.01),但各細胞組間相互比較差異無顯著性意義;5×109 L-1組和5×1010 L-1組的新生牙槽骨量與 5×108 L-1組和對照組相比差異有顯著性 (P < 0.05),但前兩組間和後兩組間比較則差異無顯著性意義.結論:骨髓間充質榦細胞移植能促進牙根分扠病變區的牙週組織再生,其作用與細胞的接種密度有關.
배경:목전림상상II도근분차병변구아주조직적재생잉시일항난제.목적:비교불동밀도자체골수간충질간세포수복II도아근분차병변구아주조직결손적능력.설계:수궤대조관찰실험.단위:복건의과대학부속구강의원실험실화복주총의원동물실험과.재료:실험우2005-07/2006-09재해방군남경군구복주총의원동물실험과화복건의과대학부속구강의원실험실완성.6지1세반웅성Beagle견유해방군남경군구복주총의원동물실험과제공,실험과정중대동물처치부합동물윤리학표준.실험중선용Bio-Gide효원막화BME-10X효원막.방법:재견적하합제2,3전마아화제1마아협측근분차처제비만성II도아근분차병변모형.종6지18월령적Beagle견추취자체골수간충질간세포.응용5×108 L-1,5×109 L-1,5×1010 L-1적골수간충질간세포복합BME-10X효원막이식치료아근분차병변,표면복개Bi0-Gide효원막,단순Bi0-Gide효원막조위대조조.채용OLYPUS IX 71현미조상계통화OLYSIA BioAutoCell연건계산신생아골질장도적백분비화신생아조골면적적백분비.주요관찰지표:소목정-이홍염색관찰병측량아주조직재생(신생아골질장도화신생아조골면적적백분비)정황.결과:대조조적신생아골질장도화신생아조골면적적백분비분별위:(51.5±5.6)%화(27.1±7.7)%;5×108 L-1골수간충질간세포조위:(84.8±8.9)%화(30.6±7.7)%; 5×109 L-1골수간충질간세포조위:(91.8±5.2)%화(68.3±11.4)%;5×1010 L-1골수간충질간세포조위:(88.8±7.2)%화(78.5±12.7)%.세포조적신생아골질량여대조조상비,차이유현저성의의(P < 0.01),단각세포조간상호비교차이무현저성의의;5×109 L-1조화5×1010 L-1조적신생아조골량여 5×108 L-1조화대조조상비차이유현저성 (P < 0.05),단전량조간화후량조간비교칙차이무현저성의의.결론:골수간충질간세포이식능촉진아근분차병변구적아주조직재생,기작용여세포적접충밀도유관.
BACKGROUND: Regeneration of type Ⅱ furcation defects of periodontal tissues is still a great clinical challenge. OBJECTIVE: To compare different densities of autologous bone marrow mesenchymal stem cells (auto-BMSCs) for repairing canine experimental class Ⅱ furcation defects of periodontal tissues. DESIGN: A randomized controlled trial. SETTING: Laboratory in Stomatological Hospital Affiliated to Fujian Medical University and Department of Animal Experiment in Fuzhou General Hospital. MATERIALS: Experiments were performed at the Laboratory in Stomatological Hospital Affiliated to Fujian Medical University and Department of Animal Experiment in Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA from July 2005 to September 2006. Six 18-month Beagle dogs were provided by Department of Animal Experiment in Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA. Animal intervention met animal ethical standards. Bio-Gide collagen membrane and BME-10X collagen membrane were used in the study.METHODS: Class Ⅱ furcation defects were induced surgically on the buccal side of canine mandibular second and third premolar (P2, P3) and first molar (M1). The ex vivo expanded auto-BMSCs from six 18-month Beagle dogs were seeded in BME-10X collagen membranes at cell density of 5×108 L-1，5×109 L-1，5×1010 L-1, and delivered into experimental class Ⅱ furcation defects, underneath a Bio-Gide membrane. Bio-Gide membrane alone was used as a control. The percentage of new cementum length and percentage of new alveolar bone area were measured on OLYPUS IX 71 inverted research microscope and OLYSIA BioAutoCell software in a computer.MAIN OUTCOME MEASURES: Each specimen was stained with hematoxylin and eosin. The lengths of new cementum and the area of new alveolar bone were calculated.RESULTS: The percentage of newly formed cementum length and the percentage of newly formed alveolar bone area were (51.5±5.6)% and (27.1±7.7)% in the control group,（84.8±8.9）% and（30.6±7.7）% in the 5×108 L-1 BMSCs group, (91.8±5.2)% and (68.3±11.4)% in the 5×109 L-1 BMSCs group and (88.8±7.2)% and (78.5±12.7)% in the 5×1010 L-1 BMSCs group. There were significant differences when comparing the BMSCs groups to the control group (P < 0.01), but there was no significant difference in each BMSCs group. There were significant differences in the percentage of newly formed alveolar bone when comparing the 5×109 L-1 and 5×1010 L-1 BMSCs groups to 5×108 L-1 BMSCs group and control group (P < 0.05), but there was no significantly difference between the first two groups, and neither was the later.CONCLUSION:Periodontal regeneration can be induced by BMSCs transplantation. The mechanism of regeneration is associated with inoculated density.
