CAJ | 학술논문

采用硫酸铵沉淀、有机溶剂分离、Sephadex G-25分子筛层析等实验方法,从文蛤肉中提取了一种低分子量的多肽,命名为Mer2.本文以溴化二苯偶氮盐(MTT)法检测Mer2对体外培养的癌细胞的抑制作用.结果表明Mer2对体外培养的人肝癌细胞株(HepG2)、宫颈癌细胞株(Hela)、胆管癌细胞株(QBC939)、肺癌细胞株(SPC-A-1和LTEP-a-2)的生长均有很强的抑制作用,且抑制效果随着Mer2含量的增高和处理时间的延长而增强,证明Mer2具有广谱的抗癌活性.其中Mer2对人肝癌HepG_2细胞株抑制作用最为显著,光学显微镜观察经Mer2细胞培养液处理后的细胞形态发生明显的改变,流式细胞仪实验的结果表明处理后的细胞周期发生明显的改变,并在G_0/G_1期前出现凋亡峰.
채용류산안침정、유궤용제분리、Sephadex G-25분자사층석등실험방법,종문합육중제취료일충저분자량적다태,명명위Mer2.본문이추화이분우담염(MTT)법검측Mer2대체외배양적암세포적억제작용.결과표명Mer2대체외배양적인간암세포주(HepG2)、궁경암세포주(Hela)、담관암세포주(QBC939)、폐암세포주(SPC-A-1화LTEP-a-2)적생장균유흔강적억제작용,차억제효과수착Mer2함량적증고화처리시간적연장이증강,증명Mer2구유엄보적항암활성.기중Mer2대인간암HepG_2세포주억제작용최위현저,광학현미경관찰경Mer2세포배양액처리후적세포형태발생명현적개변,류식세포의실험적결과표명처리후적세포주기발생명현적개변,병재G_0/G_1기전출현조망봉.
An anti-cancer peptide was purified from Mercenaria(Meretrix meretrix)by methods of fragmentation,organic precipitation and column chromatogram (Sephadex G-25).Finally,the anticancer peptide was obtained and named as Mer2.The antitumor activity and stability of Mer2 were preliminarily determined by MTT method in vitro.It shows that Mer2 inhibited significantly the growth of human cancer cells including HepG_2,HeLa,QBC939,SPC-A-1 and LTEP-a-2 in a dose-dependent manner in vitro,and had a more remarkable inhibitory effect on liver cancer cell strain HepG_2.The cell morphology of HepG_2 treated by the peptide Mer2 was changed distinctly under the LM and the proliferation of HepG_2 cells through induced-apoptosis was changed clearly showed by flow cytometry.