CAJ | 학술논문

目的:调查并分析佛山地区无偿献血者Rh 血型分布情况.方法:使用抗-D、抗-C、抗-c、抗-E和抗-e血清进行Rh血型分型,对RhD抗原筛查阴性的标本使用3批不同厂家 IgG 抗-D 血清采用抗人球蛋白法进行确认.根据实验结果,计算Rh 表现型、基因、单倍型频率,并进行Hardy-Weinberg 吻合度检验.结果:佛山地区无偿献血者Rh血型表现型频率特点为CCDee＞CcDEe＞CcDee＞ccDEE＞ccDEe＞CCDEe＞CcDEE＞ccdee＞ccDee,Ccdee＞CCdee,ccdEe＞CCDEE,CCdEE,CCdEe,CcdEE,CcdEe,ccdEE;单倍型频率特点为CDe＞cDE＞cDe＞CDE;cde＞Cde＞cdE＞CdE;基因频率特点为D＞d,C＞c,e＞E;D(-)表型频率为0.379%,d基因频率为0.061.本文Rh血型单倍型及基因的观察值与期望值差异无统计学意义(P>0.05),Hardy-Weinberg吻合度检验良好.结论:本调查数据符合群体遗传学中的Hardy-Weinberg定律,结果可靠;佛山地区无偿献血人群的Rh血型分布,既有地域特点,也有汉族人群的共性;本研究结果对指导佛山地区科学地进行无偿献血招募及合理地储存血液,提高血站为稀有血型受血者及时供血的能力,具有重要的意义.
목적:조사병분석불산지구무상헌혈자Rh 혈형분포정황.방법:사용항-D、항-C、항-c、항-E화항-e혈청진행Rh혈형분형,대RhD항원사사음성적표본사용3비불동엄가 IgG 항-D 혈청채용항인구단백법진행학인.근거실험결과,계산Rh 표현형、기인、단배형빈솔,병진행Hardy-Weinberg 문합도검험.결과:불산지구무상헌혈자Rh혈형표현형빈솔특점위CCDee＞CcDEe＞CcDee＞ccDEE＞ccDEe＞CCDEe＞CcDEE＞ccdee＞ccDee,Ccdee＞CCdee,ccdEe＞CCDEE,CCdEE,CCdEe,CcdEE,CcdEe,ccdEE;단배형빈솔특점위CDe＞cDE＞cDe＞CDE;cde＞Cde＞cdE＞CdE;기인빈솔특점위D＞d,C＞c,e＞E;D(-)표형빈솔위0.379%,d기인빈솔위0.061.본문Rh혈형단배형급기인적관찰치여기망치차이무통계학의의(P>0.05),Hardy-Weinberg문합도검험량호.결론:본조사수거부합군체유전학중적Hardy-Weinberg정률,결과가고;불산지구무상헌혈인군적Rh혈형분포,기유지역특점,야유한족인군적공성;본연구결과대지도불산지구과학지진행무상헌혈초모급합리지저존혈액,제고혈참위희유혈형수혈자급시공혈적능력,구유중요적의의.
Objective:To investigate and analysis the distribution situations of Rh blood group system from voluntary blood donors in Foshan area.Methods:Anti-D,anti-C,anti-c,anti-E and anti-e reagents were used to identify Rh blood group system and the RhD(-) was confirmed.The phenotypes,haplotypes and corresponding genotypes frequencies were calculated and the datas were analyzed based on Hardy-Weinberg law.Results:The characteristics of phenotype frequencies in the voluntary blood donors in Foshan area was CCDee＞CcDEe＞CcDee＞ccDEE＞ccDEe＞CCDEe＞CcDEE＞ccdee＞ccDee,Ccdee＞CCdee,ccdEe＞CCDEE,CCdEE,CCdEe,CcdEE,CcdEe and ccdEE.The characteristics of haplotypes frequencies was CDe＞cDE＞cDe＞CDE,de＞Cde＞cdE＞CdE.The characteristics of genes frequencies was D＞d,C＞c,e＞E.The frequency of RhD(-)phenotype was 0.379%.The gene frequency of d was 0.061.The observed value and desired value of the haplotypes and corresponding genotypes were not statistically significant(P>0.05).The data were analyzed well based on Hardy-Weinberg law.Conclusion:The result is reliable according to Hardy-Weinberg law.The distribution of Rh blood group system from voluntary blood donors in Foshan area has geographic and general characteristics.The result is important in guiding the recruitment of voluntary blood donors and banking blood reasonably and enhancing the abilitiy of offering blood for the recipients of rare blood group.