CAJ | 학술논문

[目的]对日本落叶松体细胞胚胎发生进行系统研究.[方法]以日本落叶松5个品种的未成熟合子胚为外植体,研究基本培养基、品种基因型、杂交系和纯系品种对日本落叶松愈伤组织诱导的影响,以及ABA对体细胞胚成熟及植株再生的影响.[结果]通过对日本落叶松胚性愈伤组织的诱导和体细胞胚的成熟培养获得再生植株.2种基本培养基中,DCR对胚性愈伤组织的诱导频率最高,达54.0%;落叶松品种之间的愈伤组织诱导率有差异,以杂交品种愈伤组织的诱导率最高,达71.4%,优于纯系的落叶松品种(27.3%～50.0%);在体细胞胚的成熟培养过程中,ABA可严重影响体细胞胚的发育,并影响到其以后的萌发能力和植株再生.[结论]杂交品系更有利于胚性愈伤组织诱导,ABA 对日本落叶松体细胞胚胎发育起重要作用.
[목적]대일본락협송체세포배태발생진행계통연구.[방법]이일본락협송5개품충적미성숙합자배위외식체,연구기본배양기、품충기인형、잡교계화순계품충대일본락협송유상조직유도적영향,이급ABA대체세포배성숙급식주재생적영향.[결과]통과대일본락협송배성유상조직적유도화체세포배적성숙배양획득재생식주.2충기본배양기중,DCR대배성유상조직적유도빈솔최고,체54.0%;락협송품충지간적유상조직유도솔유차이,이잡교품충유상조직적유도솔최고,체71.4%,우우순계적락협송품충(27.3%～50.0%);재체세포배적성숙배양과정중,ABA가엄중영향체세포배적발육,병영향도기이후적맹발능력화식주재생.[결론]잡교품계경유리우배성유상조직유도,ABA 대일본락협송체세포배태발육기중요작용.
[Objective] The aim was to study the somatic embryogenesis of Larix leptolepis systematically. [Method] With premature zygotic embryos of 5 L. leptolepis cultivars as explants, the effects of basic medium, cultivar genotypes, hybrid and pure lines cultivars on the callus induction of L. leptolepis and the effects of ABA on somatic maturation and plant regeneration were studied. [Result] The regenerated plants were obtained through embryogenic callus induction of L. leptolepis and maturation culture of somatic embryo. In the 2 basic media, the induction frequency of DCR on embryogenic calli was highest, being up to 54.0%;the induction frequencies of callus had difference among L. leptolepis cultivars and that of hybrid cultivars was highest, being up to 71.4% and superior to that of pure line L. leptolepis cultivars (The difference is 27.3%-50.0%.);in the maturation culture process of somatic embryos, ABA could affect the development of somatic embryos strongly and affect their subsequent germination ability and plant regeneration. [Conclusion] The hybrid lines were more favorable to the induction of embryogenic calli and ABA played important role on the development of somatic embryogenesis of L. leptolepis.