CAJ | 학술논문

三维荧光光谱技术通过在不同激发波长下扫描发射光谱获得荧光强度变化信息,由于其灵敏度高,选择性好,被广泛用于环境中污染物的监测.利用该方法研究3种酚类化合物的荧光光谱特性,在激发波长为240～360 nm,发射波长为260～500 nm范围内,确定了苯酚、间甲酚和麝香草酚的荧光峰位置分别为272/300,274/300和276/304 nm.由于3种酚类物质为同系物,结构相似,因此得到的激发光谱和发射光谱在形状上极为相似.工作液浓度在0.02～1.0 mg·L-1范围内,3种酚类物质的浓度与荧光强度之间均呈现较好的线性关系,且检出限达到1 μg·L-1.实验结果表明,用三维荧光光谱法町对3种酚类化合物进行定性和定量分析.
삼유형광광보기술통과재불동격발파장하소묘발사광보획득형광강도변화신식,유우기령민도고,선택성호,피엄범용우배경중오염물적감측.이용해방법연구3충분류화합물적형광광보특성,재격발파장위240～360 nm,발사파장위260～500 nm범위내,학정료분분、간갑분화사향초분적형광봉위치분별위272/300,274/300화276/304 nm.유우3충분류물질위동계물,결구상사,인차득도적격발광보화발사광보재형상상겁위상사.공작액농도재0.02～1.0 mg·L-1범위내,3충분류물질적농도여형광강도지간균정현교호적선성관계,차검출한체도1 μg·L-1.실험결과표명,용삼유형광광보법정대3충분류화합물진행정성화정량분석.
The fluorescence intensity information was collected by scanning its fluorescence spectra at different excitation wavelengths.Based on its high sensitivity and selectivity,excitation-emission fluorescence can be widely used for detection of pollutants in the environment.The characterizations of the three phenolic compounds were investigated by this method,and the fluorescence peaks of phenol,m-cresol and thymol were confirmed at 272/300 nm,274/300 nm and 276/304 nm when the excitation and emission wavelength were set in the range of 240-360 nm and 260-500 nm respectively.The excitation and emission spectra of the three phenolic compounds are very similar because of their analogical structure.The intensity of the spectrum has a good linear relationship with the concentration when the solution concentration is between 0.02 and 1.0 mg·L-1,and the limits of detection can reach at 1 μg·L-1.Results show that the three compounds can be analyzed qualitatively and quantitatively by excitation-emission fluorescence.