CAJ | 학술논문

目的探讨血清白细胞介素-17(IL-17)、白三烯B4(LTB4)及总IgE水平在支气管哮喘发病中的作用及其关系.方法研究组选择哮喘急性发作患儿60例(其中轻度组29例,中重度组31例),对照组选择健康儿童24例.应用酶联免疫吸附法检测各组儿童血清IL-17、LTB4水平,荧光酶联免疫法检测血清总IgE水平,哮喘患儿进行肺功能检测.全自动血液分析仪进行外周血中性粒细胞计数.结果 (1)哮喘轻度组、中重度组与对照组血清IL-17水平分别为(1.15±0.10)μg/L、(2.80±2.30)μg/L、(0.83±0.10)μg/L；LTB4水平分别为(2.22±1.01)μg/L、(8.79±9.36)μg/L、(1.94±1.13)μg/L；IgE 水平分别为(123.70±86.94)μg/L、(322.27±332.28)μg/L、(24.27±7.64)μg/L,组间比较差异均有统计学意义(P＜0.001)；(2)哮喘轻度组、中重度组与对照组血清中性粒细胞百分比分别为(55.06±11.15)％、(64.44±11.87)％、(47.96±13.52)％,淋巴细胞百分比分别为(42.20±11.04)％、(33.93±10.02)％、(49.65±13.02)％,组间比较差异有统计学意义(P＜0.05)；(3)哮喘患儿血清IL-17与IgE水平呈正相关(P=0.004),哮喘患儿血清LTB4与IgE水平呈正相关(P=0.011),哮喘患儿血清IL-17与LTB4水平呈正相关(P=0.000)；(4)哮喘患儿血清IL-17、LTB4水平与FEV1(P=0.000)及PEF(P=0.000)均呈负相关,与中性粒细胞百分比呈正相关(P=0.000).结论 IL-17、LTB4及IgE在哮喘的急性发作及加重中起着重要作用,在哮喘发病中彼此相互影响,共同参与哮喘的病理生理改变.
목적 탐토혈청백세포개소-17(IL-17)、백삼희B4(LTB4)급총IgE수평재지기관효천발병중적작용급기관계.방법 연구조선택효천급성발작환인60례(기중경도조29례,중중도조31례),대조조선택건강인동24례.응용매련면역흡부법검측각조인동혈청IL-17、LTB4수평,형광매련면역법검측혈청총IgE수평,효천환인진행폐공능검측.전자동혈액분석의진행외주혈중성립세포계수.결과 (1)효천경도조、중중도조여대조조혈청IL-17수평분별위(1.15±0.10)μg/L、(2.80±2.30)μg/L、(0.83±0.10)μg/L；LTB4수평분별위(2.22±1.01)μg/L、(8.79±9.36)μg/L、(1.94±1.13)μg/L；IgE 수평분별위(123.70±86.94)μg/L、(322.27±332.28)μg/L、(24.27±7.64)μg/L,조간비교차이균유통계학의의(P＜0.001)；(2)효천경도조、중중도조여대조조혈청중성립세포백분비분별위(55.06±11.15)％、(64.44±11.87)％、(47.96±13.52)％,림파세포백분비분별위(42.20±11.04)％、(33.93±10.02)％、(49.65±13.02)％,조간비교차이유통계학의의(P＜0.05)；(3)효천환인혈청IL-17여IgE수평정정상관(P=0.004),효천환인혈청LTB4여IgE수평정정상관(P=0.011),효천환인혈청IL-17여LTB4수평정정상관(P=0.000)；(4)효천환인혈청IL-17、LTB4수평여FEV1(P=0.000)급PEF(P=0.000)균정부상관,여중성립세포백분비정정상관(P=0.000).결론 IL-17、LTB4급IgE재효천적급성발작급가중중기착중요작용,재효천발병중피차상호영향,공동삼여효천적병리생리개변.
Objective To explore the impact of levels of serum IL-17,Leukotriene B4 and IgE on pathogenesis of childhood asthma.Methods Totally 60 children with asthma acute exacerbation ( 29 children with mild asthma,31 children with moderate-severe asthma) were selected as study group,24 healthy children were selected as control group.Serum IL-17 and LTB4 were measured with euryzemLinked immunosorbent assay,serum IgE was determined with enzyme-linked fluoroimmuneassay by pharmacia CAP Sytem,PMN was determined with automatic blood analyser,pulmonary function was measured in the study group.Results ( 1 ) The level of serum IL-17 ( 1.15 ± 0.10 μg/L,2.80 ± 2.30 μg/L,0.83 ± 0.10 μg/L),LTB4 (2.22 ± 1.01 μg/L,8.79 ± 9.36 μg/L,1.94 ± 1.13 μg/L) and IgE( 123.70 ±86.94 μg/L,322.27 ±332.28 μg/L,24.27 ±7.64 μg/L) were significantly different among mild asthma group,moderate-severe asthma group and control group( P ＜ 0.001 ).( 2 )The N％ of mild asthma group,moderate-severe asthma group and study group were( 55.06 ± 1 1.15 ) ％,( 64.44± 11.87)％,(47.96 ± 13.52)％,L％ were(42.20 ± 11.04)％,(33.93 ± 10.02)％,(49.65 ± 13.02)％,and there were significant differences in N％ and L％ between study group and control group( P ＜ 0.05 ).( 3 ) There were significant positive correlations between the serum IL-17 levels and IgE,LTB4 and IgE,IL-17 and LTB4 in asthmatic children( P ＜0.05).(4) There were significant negative correlcations between the level of serum IL-17,LTB4 and FEVI,PEF( P ＜0.001 ).There were significant positive correlations between serum IL-17,LTB4 and N％ (P ＜0.001 ).(5)There were not correlations between the level of serum IgE and FEV1,PEF and N％in asthmatic children( P ＞0.05 ).Conclusion The levels of serum IL-17,LTB4 and IgE participated in pathogenesis on asthmatic children patients.