国际流行病学传染病学杂志
國際流行病學傳染病學雜誌
국제류행병학전염병학잡지
INTERNATIONAL JOURNAL OF EPIDEMIOLOGY AND INFECTIOUS DISEASE
2008年
5期
297-300
,共4页
丁水军%孙永祥%汪皓秋%傅丹青%戴城钢
丁水軍%孫永祥%汪皓鞦%傅丹青%戴城鋼
정수군%손영상%왕호추%부단청%대성강
志贺菌%4c亚型%ipaH%脉冲场凝胶电泳%分型
誌賀菌%4c亞型%ipaH%脈遲場凝膠電泳%分型
지하균%4c아형%ipaH%맥충장응효전영%분형
Shigella%4c subtype%ipaH%Pulse-field gel electrophoresis%Typing
目的 了解杭州地区2003-2007年从爆发和散发细菌性痢疾患者中分离之福氏志贺菌4c亚型(F4c)菌株的分子生物学特征.方法 对24株F4c菌株以生化反应和血清分型进行鉴定,利用PCR法检测侵袭性质粒抗原H基因(ipaH)及脉冲场凝胶电泳(PFGE)进行分型分析.结果 24株菌株全部为F4c,均具有ipaH毒力基因.用PFGE分型方法将24株菌株分成17种不同的带型,Dice系数为0.71～1.00.2005年、2006年、2003年三次爆发(分别简称为爆发1、2、3)中各自分离到的菌株基因型紧密相关.三次爆发中的部分菌株可能存在一定的相关性(Dice系数>0.8).散发菌株和爆发1、3菌株间的距离较远(Dice系数<0.8),和爆发2菌株间可能存在一定的相关性(Dice系数>0.8).结论 F4c菌株具有ipaH毒力基因,PFGE提示杭州地区近年F4c三起爆发菌株可能属共同的克隆群.
目的 瞭解杭州地區2003-2007年從爆髮和散髮細菌性痢疾患者中分離之福氏誌賀菌4c亞型(F4c)菌株的分子生物學特徵.方法 對24株F4c菌株以生化反應和血清分型進行鑒定,利用PCR法檢測侵襲性質粒抗原H基因(ipaH)及脈遲場凝膠電泳(PFGE)進行分型分析.結果 24株菌株全部為F4c,均具有ipaH毒力基因.用PFGE分型方法將24株菌株分成17種不同的帶型,Dice繫數為0.71～1.00.2005年、2006年、2003年三次爆髮(分彆簡稱為爆髮1、2、3)中各自分離到的菌株基因型緊密相關.三次爆髮中的部分菌株可能存在一定的相關性(Dice繫數>0.8).散髮菌株和爆髮1、3菌株間的距離較遠(Dice繫數<0.8),和爆髮2菌株間可能存在一定的相關性(Dice繫數>0.8).結論 F4c菌株具有ipaH毒力基因,PFGE提示杭州地區近年F4c三起爆髮菌株可能屬共同的剋隆群.
목적 료해항주지구2003-2007년종폭발화산발세균성이질환자중분리지복씨지하균4c아형(F4c)균주적분자생물학특정.방법 대24주F4c균주이생화반응화혈청분형진행감정,이용PCR법검측침습성질립항원H기인(ipaH)급맥충장응효전영(PFGE)진행분형분석.결과 24주균주전부위F4c,균구유ipaH독력기인.용PFGE분형방법장24주균주분성17충불동적대형,Dice계수위0.71～1.00.2005년、2006년、2003년삼차폭발(분별간칭위폭발1、2、3)중각자분리도적균주기인형긴밀상관.삼차폭발중적부분균주가능존재일정적상관성(Dice계수>0.8).산발균주화폭발1、3균주간적거리교원(Dice계수<0.8),화폭발2균주간가능존재일정적상관성(Dice계수>0.8).결론 F4c균주구유ipaH독력기인,PFGE제시항주지구근년F4c삼기폭발균주가능속공동적극륭군.
Objective To analysis the molecular biological characteristics of Shigella flexneri 4c isolates from patients of shigellosis outbreak and sporadic diaxrbea in Hangzhou during 2003-2007. Method 24 S. flexneri 4c isolates were identified by biochemical tests and serotyping. Among these isolates, invasive plasmid antigen H gene (ipaH) was examined by PCR, and molecular typing was performed by Pulse-field gel electrophoresis(PFGE). Results All 24 isolates were serotyped as S. flexneri 4c and were positive for ipaH gene. A total of 17 different PFGE patterns were identified among these 24 isolates with Dice coefficient varying from 0.71 to 1.00. On the basis of PFGE patterns, S. flexneri 4c isolates from each of three shigellesis outbreaks were closely related. Some isolates from three outbreaks (outbreak in 2005,2006 and 2003 year which named as outbreak 1,2,3 respectively) were possibly related (Dice coefficient > 0.8). The sporadic isolates were demonstrated to be distinct from the isolates from two outbreaks (outbreak 1,3) ( Dice coefficient <0.8). However, the sporadic isolates were possibly related to isolates from outbreak 2 (Dice coefficient > 0.8). Conclusions All S. flexneri 4c in Hangzhou possess ipaH. The S. flexneri 4c isolates from three recent outbreaks in Hangzhou may belong to the same clone.
