中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2007年
4期
210-212
,共3页
刘慧姝%宋小飞%袁文常%郝荣增
劉慧姝%宋小飛%袁文常%郝榮增
류혜주%송소비%원문상%학영증
水通道蛋白3%胎盘%胎膜%体液%基因表达
水通道蛋白3%胎盤%胎膜%體液%基因錶達
수통도단백3%태반%태막%체액%기인표체
Aquaporin 3%Placenta%Fetal membranes%Body fluids%Gene expression
目的 研究正常妊娠晚期人胎盘和胎膜水通道蛋白3(AQP3)的表达及分布.方法 收集5例正常足月妊娠剖宫分娩的胎盘和胎膜样本,用RT-PCR测定AQP3 mRNA在胎盘和胎膜组织中的表达;用Western印迹和免疫组织化学方法检测AQP3蛋白质水平在胎盘和胎膜的表达.结果 RT-PCR显示AQP3 mRNA在胎盘和胎膜组织均有表达.Western印迹结果显示胎盘组织在29 000左右有一特异性条带.免疫组织化学结果显示AQP3表达于合体滋养细胞,而在羊膜上皮细胞未见表达.结论 AQP3在胎盘母儿液体平衡中可能发挥重要作用.
目的 研究正常妊娠晚期人胎盤和胎膜水通道蛋白3(AQP3)的錶達及分佈.方法 收集5例正常足月妊娠剖宮分娩的胎盤和胎膜樣本,用RT-PCR測定AQP3 mRNA在胎盤和胎膜組織中的錶達;用Western印跡和免疫組織化學方法檢測AQP3蛋白質水平在胎盤和胎膜的錶達.結果 RT-PCR顯示AQP3 mRNA在胎盤和胎膜組織均有錶達.Western印跡結果顯示胎盤組織在29 000左右有一特異性條帶.免疫組織化學結果顯示AQP3錶達于閤體滋養細胞,而在羊膜上皮細胞未見錶達.結論 AQP3在胎盤母兒液體平衡中可能髮揮重要作用.
목적 연구정상임신만기인태반화태막수통도단백3(AQP3)적표체급분포.방법 수집5례정상족월임신부궁분면적태반화태막양본,용RT-PCR측정AQP3 mRNA재태반화태막조직중적표체;용Western인적화면역조직화학방법검측AQP3단백질수평재태반화태막적표체.결과 RT-PCR현시AQP3 mRNA재태반화태막조직균유표체.Western인적결과현시태반조직재29 000좌우유일특이성조대.면역조직화학결과현시AQP3표체우합체자양세포,이재양막상피세포미견표체.결론 AQP3재태반모인액체평형중가능발휘중요작용.
Objective To investigate the expression and distribution of aquaporin 3(AQP3)in normal human placenta and fetal membranes.Methods Placenta and fetal membrane were collected from 5 normal women with intact membranes undergone elective cesarean sections at term.Expression and localization of AQP3 was detected by reverse transcriptase-polymerase chain reaction(RT-PCR),Western blot analysis and immunohistochemistry.Results AQP3 messenger RNA was found in placenta and fetal membrane.Western blot analysis also yielded positive results for placenta and fetal membrane with specific band site around 29 000.Immunohistochemical localization showed AQP3 Was present at syncytotrophoblast of placenta and there was no signal staining at epithelial cell of amnion.Condusion AQP3 may play an important role in maternal-fetal fluid balance.
