CAJ | 학술논문

目的观察大鼠骨髓多能成体祖细胞(mulipotent adult progenitor cells,MAPCs)是否可在体向皮肤组织细胞分化.方法采用免疫磁性分选技术(MACS)获取MAPCs;通过皮肤受损的C57BL/6小鼠与免疫缺陷性裸鼠的尾静脉注入MAPCs,免疫组织化学技术检测愈合皮肤中大鼠MHC I类抗原的表达.结果 MHC I抗原在C57BL/6小鼠愈合皮肤处的毛囊皮脂腺周围及毛囊外根鞘与皮脂腺交界处均有阳性表达细胞;裸鼠实验组愈合皮肤内出现毛囊样结构,在表皮基底部及部分毛囊样结构中有MHC I抗原阳性表达的细胞.结论在皮肤组织损伤的条件下,MAPCs可迁移至受损皮肤表面及皮肤附属器毛囊周围,并通过转化为表皮细胞参与受损皮肤的愈合.
목적 관찰대서골수다능성체조세포(mulipotent adult progenitor cells,MAPCs)시부가재체향피부조직세포분화.방법 채용면역자성분선기술(MACS)획취MAPCs;통과피부수손적C57BL/6소서여면역결함성라서적미정맥주입MAPCs,면역조직화학기술검측유합피부중대서MHC I류항원적표체.결과 MHC I항원재C57BL/6소서유합피부처적모낭피지선주위급모낭외근초여피지선교계처균유양성표체세포;라서실험조유합피부내출현모낭양결구,재표피기저부급부분모낭양결구중유MHC I항원양성표체적세포.결론 재피부조직손상적조건하,MAPCs가천이지수손피부표면급피부부속기모낭주위,병통과전화위표피세포삼여수손피부적유합.
Objective To observe the differentiation of bone marrow multipotent adult progenitor cells(MAPCs)into skin tissue cells of rats in vivo. Methods Magnetic activated cell sorting (MACS)was used to remove MAPCs from bone marrow of male rats through negative screening.Tail vein injection with combined with MAPCs Was done in C57BL/6 mice with skin wound and nude mice with immunodeficieney.Immunohistochemical staining was used to examine the expression of MHCI antigen in the healed skin of donor SD rats. Results Positive MHCI cells were found in the epidermal fundus and Some hair follicle-like structures of the healed skin of C57BL/6 mice.Hair follicle-like structure appeared in the healed skin of nude mice group,in which positive MHCI cells were found in the basal epidermal and some hair follicle-like structures. Conclusions During skin damage,MAPCs can migrate to the injured skin area and skin adnexa hair foilicle area,transform into epidermal cells and hence participate in the healing of the wound skin.