中华肝胆外科杂志
中華肝膽外科雜誌
중화간담외과잡지
CHINESE JOURNAL OF HEPATOBILIARY SURGERY
2010年
7期
504-507
,共4页
宿华威%孙世波%孙铁为%吴德全
宿華威%孫世波%孫鐵為%吳德全
숙화위%손세파%손철위%오덕전
肝移植%血红素氧合酶-1%缺血再灌注损伤
肝移植%血紅素氧閤酶-1%缺血再灌註損傷
간이식%혈홍소양합매-1%결혈재관주손상
Liver transplantation%Heme oxygenase-1%Ischemia/reperfusion injury
目的 研究移植肝脏血红素氧合酶(HO-1)表达水平与缺血再灌注损伤和移植术后肝脏功能的关系.方法 研究28例人类临床原位肝脏移植,根据供肝血红素氧合酶(HO-1)表达的平均值将供肝分为两组:移植前供肝HO-1高表达组和移植前供肝HO-1低表达组.比较两组移植术后血浆AST、ALT水平、胆汁中胆盐含量以及术前术后HO-1 mRNA和蛋白表达情况.结果 再灌注后移植术前HO-1低表达组的HO-1 mRNA表达显著增加,而高表达组HO-1 mRNA表达却有所下降.肝脏移植后,术前HO-1低表达组与高表达组相比,血浆转氨酶显著降低,胆汁中胆盐含量明显高于后者.结论 移植术前HO-1低表达组供肝在再灌注过程中能够进一步诱导HO-1表达,与高表达组供肝相比其所遭受的缺血再灌注损伤较轻,移植术后肝脏功能较好.移植过程中HO-1表达的增强要比移植前HO-1高表达更具有细胞保护作用.免疫荧光染色证实枯否细胞是人类肝脏表达HO-1的主要部位.
目的 研究移植肝髒血紅素氧閤酶(HO-1)錶達水平與缺血再灌註損傷和移植術後肝髒功能的關繫.方法 研究28例人類臨床原位肝髒移植,根據供肝血紅素氧閤酶(HO-1)錶達的平均值將供肝分為兩組:移植前供肝HO-1高錶達組和移植前供肝HO-1低錶達組.比較兩組移植術後血漿AST、ALT水平、膽汁中膽鹽含量以及術前術後HO-1 mRNA和蛋白錶達情況.結果 再灌註後移植術前HO-1低錶達組的HO-1 mRNA錶達顯著增加,而高錶達組HO-1 mRNA錶達卻有所下降.肝髒移植後,術前HO-1低錶達組與高錶達組相比,血漿轉氨酶顯著降低,膽汁中膽鹽含量明顯高于後者.結論 移植術前HO-1低錶達組供肝在再灌註過程中能夠進一步誘導HO-1錶達,與高錶達組供肝相比其所遭受的缺血再灌註損傷較輕,移植術後肝髒功能較好.移植過程中HO-1錶達的增彊要比移植前HO-1高錶達更具有細胞保護作用.免疫熒光染色證實枯否細胞是人類肝髒錶達HO-1的主要部位.
목적 연구이식간장혈홍소양합매(HO-1)표체수평여결혈재관주손상화이식술후간장공능적관계.방법 연구28례인류림상원위간장이식,근거공간혈홍소양합매(HO-1)표체적평균치장공간분위량조:이식전공간HO-1고표체조화이식전공간HO-1저표체조.비교량조이식술후혈장AST、ALT수평、담즙중담염함량이급술전술후HO-1 mRNA화단백표체정황.결과 재관주후이식술전HO-1저표체조적HO-1 mRNA표체현저증가,이고표체조HO-1 mRNA표체각유소하강.간장이식후,술전HO-1저표체조여고표체조상비,혈장전안매현저강저,담즙중담염함량명현고우후자.결론 이식술전HO-1저표체조공간재재관주과정중능구진일보유도HO-1표체,여고표체조공간상비기소조수적결혈재관주손상교경,이식술후간장공능교호.이식과정중HO-1표체적증강요비이식전HO-1고표체경구유세포보호작용.면역형광염색증실고부세포시인류간장표체HO-1적주요부위.
Objective To explore the relation of donor liver's heme oxygenase-1 level to ische-mia/reperfusion injury and the liver function after liver transplantation. Methods Twenty-eight pa-tients receiving orthotopic liver transplantation (OLT) were divided into two groups according to the donor liver's heme oxygenase level of high HO-1 expression group and low HO-1 expression group.The recipients' serum levels of ALT, AST, bile salt output and the expression of HO-1 mRNA were determined and compared between the 2 groups. Results HO-1 mRNA of low expression group in-creased significantly after the reperfusion. Meanwhile, the HO-1 mRNA of high expression group de-creased. The serum levels of ALT and AST of HO-1 in low expression group were much lower but bile salt concentration higher than those in high expression group. Conclusion HO-1 can be further induced in the liver of HO-1 low expression group during the reperfusion period. The I/R injury in the HO-1 low expression group was less severe than that in high expression group, but the liver function was better. The increase of HO-1 during the transplantation seems better for protecting the liver than the initial HO-1 high expression. Kupffer cells are of the main localization for HO-1 protein in human liver.
