CAJ | 학술논문

目的探讨脂氧素对子痫前期患者巨噬细胞增殖和分泌功能的影响.方法对温州医学院附属第一医院产科2007年3-7月收治的24例子痫前期孕妇(子痫前期组)和24例正常孕妇(正常妊娠组)的腹腔巨噬细胞进行体外培养.采用酶联免疫吸附试验(ELISA)测定不同浓度脂氧素(0、10、100 nmol/L)作用两组孕妇腹腔巨噬细胞48 h后,上清液中肿瘤坏死因子α(TNF-α)的水平.用细胞生长抑制实验法检测两组不同浓度脂氧素(0、10、100 nmol/L)作用24 h后的巨噬细胞增殖抑制率.结果 (1)TNF-α水平:子痫前期组0、10、100 nmol/L浓度的脂氧素作用下,TNF-α水平分别为(1867.5±47.3)、(1836.9±4.5)、(1800.5±2.7)ng/L.正常妊娠组0、10、100 nmol/L浓度的脂氧素作用下,TNF-α水平分别为(791.3±62.2)、(789.4±2.3)、(781.5±1.9)ng/L.子痫前期组TNF-α水平显著高于正常妊娠组,两组比较,差异有统计学意义(P＜0.05).脂氧素对子痫前期组TNF-α水平的影响呈剂量依赖性抑制(P＜0.05),而其对正常妊娠组TNF-α水平变化无明显影响(P＞0.05).(2)增殖抑制率:子痫前期组0、10、100 nmol/L浓度的脂氧素作用下,增殖抑制率分别为(14.8±6.3)%、(32.9±3.6)%、(36.7±3.8)%.正常妊娠组0、10、100 nmol/L浓度的脂氧素作用下,增殖抑制率分别为(16.8±6.9)%、(16.7±5.4)%、(15.9±2.1)%.子痫前期组增殖抑制率比正常妊娠组明显升高,两组比较,差异有统计学意义(P＜0.05),脂氧素呈剂量依赖性抑制子痫前期组孕妇腹腔巨噬细胞的增殖抑制率(P＜0.05),而对正常妊娠组的增殖抑制率则无明显影响(P＞0.05).结论脂氧素呈剂量依赖性地抑制子痫前期患者的巨噬细胞增殖和TNF-α分泌.
목적 탐토지양소대자간전기환자거서세포증식화분비공능적영향.방법 대온주의학원부속제일의원산과2007년3-7월수치적24례자간전기잉부(자간전기조)화24례정상잉부(정상임신조)적복강거서세포진행체외배양.채용매련면역흡부시험(ELISA)측정불동농도지양소(0、10、100 nmol/L)작용량조잉부복강거서세포48 h후,상청액중종류배사인자α(TNF-α)적수평.용세포생장억제실험법검측량조불동농도지양소(0、10、100 nmol/L)작용24 h후적거서세포증식억제솔.결과 (1)TNF-α수평:자간전기조0、10、100 nmol/L농도적지양소작용하,TNF-α수평분별위(1867.5±47.3)、(1836.9±4.5)、(1800.5±2.7)ng/L.정상임신조0、10、100 nmol/L농도적지양소작용하,TNF-α수평분별위(791.3±62.2)、(789.4±2.3)、(781.5±1.9)ng/L.자간전기조TNF-α수평현저고우정상임신조,량조비교,차이유통계학의의(P＜0.05).지양소대자간전기조TNF-α수평적영향정제량의뢰성억제(P＜0.05),이기대정상임신조TNF-α수평변화무명현영향(P＞0.05).(2)증식억제솔:자간전기조0、10、100 nmol/L농도적지양소작용하,증식억제솔분별위(14.8±6.3)%、(32.9±3.6)%、(36.7±3.8)%.정상임신조0、10、100 nmol/L농도적지양소작용하,증식억제솔분별위(16.8±6.9)%、(16.7±5.4)%、(15.9±2.1)%.자간전기조증식억제솔비정상임신조명현승고,량조비교,차이유통계학의의(P＜0.05),지양소정제량의뢰성억제자간전기조잉부복강거서세포적증식억제솔(P＜0.05),이대정상임신조적증식억제솔칙무명현영향(P＞0.05).결론 지양소정제량의뢰성지억제자간전기환자적거서세포증식화TNF-α분비.
Objegtlve To study the effect of lipexins on the proliferation and secretion of peritoneal macrophages from patients with preeclampsia in vitro.Methods Peritoneal macrophages were obtained from 24 patients with preeclampsia(preeclampsia group)and 24 normal pregnant women(normal pregnant group)who were treated in the First Affiliated Hospital of Wenzhou Medical Coilege from March to July 2007.Enzyme linked immunosorbent assay (ELISA) was used to detect the concentration of tumor necrosis factor-α(TNF-α)in the supernatant of macrophages which were pulsed with lipoxins at different concentrations(0,10,100 nmol/L)in both groups after 48 hours.Methyl thiazolyl tetrazolium (MTT)assay was used to detect the inhibition rate of cell proliferation of macrophages which were pulsed with lipoxins at different concentrations(0,10,100 nmol/L)in both groups after 24 hours.Results (1)The concentration of TNF-α:the levels of TNF-α were(1867.5±47.3),(1836.9±4.5) and (1800.5±2.7)ng/L after treatment with differed concentrations of lipoxins(0,10,100 nmol/L)in preeclampsia group vs normal pregnant group[(791.3±62.2),(789.4±2.3),(781.5±1.9)ng/L].The levels of TNF-α in preeclampsia group were significantly higher than that in normal pregnant group(P＜0.05).Lipoxins significantly inhibited the concentration of TNF-α in a dose-dependent manner in preeclampsia group (P＜0.05),while it had no significant effect in normal pregnant group(P＞0.05).(2)Cell proliferation inhibition:Incubation with lipoxins produced a dose-dependent(0,10,100 nmol/L)inhibitory effect on proliferation in preeclampsia group,[(14.8±6.3)%,(32.9±3.6)%,(36.7±3.8)%],vs normal pregnant group[(16.8±6.9)%,(16.7±5.4)%,(15.9±2.1)%].The rate of cell proliferation in preeclampsia group was significantly hisher than that in normal pregnant group.Lipoxins significandy inhibited this growth(P＜0.05),while it had no significant effect in normal pregnant group(P＞0.05).Conclusion Lipoxins can inhibit the proliferation of macrophage and secretion of TNF-α in preeclampsia in a dose-dependent manner.Lipoxins may be potentially useful in prevention and treatment of preeclampsia.