中华胰腺病杂志
中華胰腺病雜誌
중화이선병잡지
CHINESE JOURNAL OF PANCREATOLOGY
2010年
2期
124-127
,共4页
邱大卫%牛立志%穆锋%彭祥%周亮%李海波%李蓉蓉%徐克成%倪嘉瓒%蒋灵芝%胡以则%郝卓芳
邱大衛%牛立誌%穆鋒%彭祥%週亮%李海波%李蓉蓉%徐剋成%倪嘉瓚%蔣靈芝%鬍以則%郝卓芳
구대위%우립지%목봉%팽상%주량%리해파%리용용%서극성%예가찬%장령지%호이칙%학탁방
胰腺肿瘤%冷冻疗法%动物实验%有效性%安全性
胰腺腫瘤%冷凍療法%動物實驗%有效性%安全性
이선종류%냉동요법%동물실험%유효성%안전성
Pancreas neoplasms%Cryotherapy%Animal experimentation%Efficacy%Safety
目的 观察胰腺冷冻前后其血液生化指标和组织变化,为胰腺癌冷冻疗法的开展提供依据.方法 将15只正常猪分为深度冷冻组(5头)、浅度冷冻组(5头)、不冷冻组(3头)和正常组(2头).麻醉后开腹暴露猪胰腺,将氩氦冷冻治疗系统探针插入胰腺,深、浅冷冻组分别采用100%和10%的氩气输出功率,冷冻温度分别为-130～-140℃和-110～120℃,形成直径15～20 mm的冰球,然后输入氦气,复温至10～20℃,维持3 min,重复冷冻-复温一个循环.不冷冻组仅插入探针,正常组仅开腹、关腹.检测实验前后血淀粉酶、IL-6、C反应蛋白(CRP)含量,第7天处死动物取胰腺行光镜及电镜观察.结果 胰腺的冷冻部位呈黑褐色坏死区,与未冷冻组织有明确界限;镜下见冷冻中心区及侧中心区有明显组织坏死,超微结构全部被破坏、消失,线粒体断嵴脱颗粒,粗面内质网脱颗粒.13头(86.7%)猪术后血淀粉酶增高,多数在第6天降至正常.5头猪(33.3%)血IL-6轻度增高.各组血清CRP水平均无明显改变.所有实验动物均健康存活直到被处死.结论 冷冻对实验动物胰腺组织有明确的致死性消融作用,该疗法安全性良好,无严重并发症.
目的 觀察胰腺冷凍前後其血液生化指標和組織變化,為胰腺癌冷凍療法的開展提供依據.方法 將15隻正常豬分為深度冷凍組(5頭)、淺度冷凍組(5頭)、不冷凍組(3頭)和正常組(2頭).痳醉後開腹暴露豬胰腺,將氬氦冷凍治療繫統探針插入胰腺,深、淺冷凍組分彆採用100%和10%的氬氣輸齣功率,冷凍溫度分彆為-130～-140℃和-110～120℃,形成直徑15～20 mm的冰毬,然後輸入氦氣,複溫至10～20℃,維持3 min,重複冷凍-複溫一箇循環.不冷凍組僅插入探針,正常組僅開腹、關腹.檢測實驗前後血澱粉酶、IL-6、C反應蛋白(CRP)含量,第7天處死動物取胰腺行光鏡及電鏡觀察.結果 胰腺的冷凍部位呈黑褐色壞死區,與未冷凍組織有明確界限;鏡下見冷凍中心區及側中心區有明顯組織壞死,超微結構全部被破壞、消失,線粒體斷嵴脫顆粒,粗麵內質網脫顆粒.13頭(86.7%)豬術後血澱粉酶增高,多數在第6天降至正常.5頭豬(33.3%)血IL-6輕度增高.各組血清CRP水平均無明顯改變.所有實驗動物均健康存活直到被處死.結論 冷凍對實驗動物胰腺組織有明確的緻死性消融作用,該療法安全性良好,無嚴重併髮癥.
목적 관찰이선냉동전후기혈액생화지표화조직변화,위이선암냉동요법적개전제공의거.방법 장15지정상저분위심도냉동조(5두)、천도냉동조(5두)、불냉동조(3두)화정상조(2두).마취후개복폭로저이선,장아양냉동치료계통탐침삽입이선,심、천냉동조분별채용100%화10%적아기수출공솔,냉동온도분별위-130～-140℃화-110～120℃,형성직경15～20 mm적빙구,연후수입양기,복온지10～20℃,유지3 min,중복냉동-복온일개순배.불냉동조부삽입탐침,정상조부개복、관복.검측실험전후혈정분매、IL-6、C반응단백(CRP)함량,제7천처사동물취이선행광경급전경관찰.결과 이선적냉동부위정흑갈색배사구,여미냉동조직유명학계한;경하견냉동중심구급측중심구유명현조직배사,초미결구전부피파배、소실,선립체단척탈과립,조면내질망탈과립.13두(86.7%)저술후혈정분매증고,다수재제6천강지정상.5두저(33.3%)혈IL-6경도증고.각조혈청CRP수평균무명현개변.소유실험동물균건강존활직도피처사.결론 냉동대실험동물이선조직유명학적치사성소융작용,해요법안전성량호,무엄중병발증.
Objective To observe the blood biochemical and histological changes before and after pancreas freezing, to provide evidence for cryosurgery for pancreatic cancer. Methods Fifteen healthy pigs were divided into deep frozen group (n = 5), shallow frozen group (n = 5), non-frozen group (n = 3) and normal group (n = 2). After anesthesia and Iaparotomy, a probe of the Argon-Helium Surgical System was inserted into the pancreas, 100% and 10% argon output power were used in deep and shallow frozen group, respectively;and the temperature were - 130 ～ - 140℃ and - 110 ～ - 120℃, respectively;which results in an ice-ball with 15 ～ 20 mm in diameter. Then helium gas was inputted to increase the temperature to 10 ～ 20℃ for three minutes;then the whole process was repeated. A probe was inserted into the pancreas in the non-frozen group only and only laparotomy was performed in non-grozen group normal group and normal group. Serum amylase, IL-6, CRP levels before and after the experiment was determined;the pigs were sacrificed at day 7 and the pancreas was harvested for light microscope and electron microscope examination. Results The frozen pancreatic tissue became pitchy necrosis zone, and it could be distinguished from non-frozen tissue;there were obvious tissue necrosis in the center and para-center of frozen area, and the ultra-structure were destroyed and disappeared, mitochondria degranulation and rough endoplasmic reticulum degrannlation were observed. Serum amylase was elevated in 13 (86.7%) pigs and most returned to normal at 6th day. Serum IL-6 was slightly elevated in 5 (33.3%) pigs. There was no significant difference among all the groups in term of serum CRP. All the pigs were alive until the time of sacrifice. Conclusions Cryosurgery has affirmative fatal ablative effects on pancreatic tissue, and it is safe with no serious complications.
