CAJ | 학술논문

目的观察小鼠肺脏基质细胞培养上清液对小鼠骨髓来源成熟树突状细胞(mDC)分化发育的影响,并探讨其影响机制.方法小鼠肺脏基质细胞培养上清液与完全培养基以1:1的比例混合后用于培养小鼠骨髓来源mDC,1周后将mDC诱导成为调节性树突状细胞(rDC).瑞氏-吉姆萨染色后观察细胞形态特征,特异性夹心酶联免疫法检测培养上清液中细胞因子白介素(IL)-10和IL 12p70的含量,流式细胞术检测细胞表型表达水平,将上述检测结果与mDC进行比较,观察肺脏基质细胞培养上清液诱导前后树突状细胞(DC)的区别.结果 rDC分泌的细胞因子IL-10高于mDC组(P＜0.01),而IL-12p70低于DC组(P＜0.01);细胞表型与mDC比较,CD11b上调(P＜0.01),CD11c、Ia、CD86下调(P＜0.01).结论肺脏基质细胞的培养上清液可诱导mDC分化发育为另一种生物学特性不同的DC亚群.
목적 관찰소서폐장기질세포배양상청액대소서골수래원성숙수돌상세포(mDC)분화발육적영향,병탐토기영향궤제.방법 소서폐장기질세포배양상청액여완전배양기이1:1적비례혼합후용우배양소서골수래원mDC,1주후장mDC유도성위조절성수돌상세포(rDC).서씨-길모살염색후관찰세포형태특정,특이성협심매련면역법검측배양상청액중세포인자백개소(IL)-10화IL 12p70적함량,류식세포술검측세포표형표체수평,장상술검측결과여mDC진행비교,관찰폐장기질세포배양상청액유도전후수돌상세포(DC)적구별.결과 rDC분비적세포인자IL-10고우mDC조(P＜0.01),이IL-12p70저우DC조(P＜0.01);세포표형여mDC비교,CD11b상조(P＜0.01),CD11c、Ia、CD86하조(P＜0.01).결론 폐장기질세포적배양상청액가유도mDC분화발육위령일충생물학특성불동적DC아군.
Objective To observe the effects of lung stromal cells on differentiation of mature dendritic cells (mDCs) deriving from mouse bone marrow. Methods The mDCs were cultured by complete medium, half of which was from the lung stromal cell cultures. One week later, the mDCs were induced into regulatory dendritic cells (rDCs). The morphological characteristics of rDCs were observed by Giemasa-Wright stain. The content of cytokines (IL-10 and IL-12p70) in the co-culture supernatants was detected by enzyme-linked immunosorbent assay. The expression level of cellular phenotype was tested with fluorescence-activated cell sorting, compared with mDCs. The difference of rDCs and mDCs was observed. Results Compared with mDCs, rDCs secreted higher level of IL-10 (P＜0.01), and lower level of IL-12p70 (P＜0. 01). In cellular phenotype, the expression of CD11b on rDCs increased (P＜0.01), while the expression of CDllc, Ⅰa and CD86 declined (P＜0.01).Conclusions Lung stromal cells microenvironment may induce the mDCs differentiate into a kind of DC with different biological characteristics.