CAJ | 학술논문

目的探讨rhIL-11和rhG-CSF对中子照射致小鼠骨髓损伤的治疗作用.方法将BALB/c小鼠130只用随机数字表法分为对照组(30只)、照射组(40只)、rhIL-11治疗组(30只)、rhIL-11+rhG-CSF治疗组(30只),用3.0 Gy中子照射,并于照射后给予600 μg·kg-1·d-1的rhIL-11和50 μg·kg-1·d-1的rhG-CSF,1次/d,共3 d;用血细胞自动分析仪、HE染色、AgNOR染色、流式细胞术、免疫组织化学染色和图像分析等,检测小鼠外周血细胞、骨髓病理形态变化、有核细胞计数、AgNOR含量、凋亡与坏死率和Bax蛋白含量的变化.结果照射组和rhIL-11治疗组于照射后6 h～3 d,小鼠外周血白细胞、骨髓有核细胞计数和AgNOR含量进行性下降,Bax蛋白于造血细胞胞质内呈阳性至强阳性表达,含量进行性增加(F=0.003,P<0.01);照射后6 h,照射组小鼠造血细胞凋亡与坏死率增加,以坏死为显著;照射后3 d,rhIL-11+rhG-CSF治疗组小鼠的骨髓有核细胞计数和AgNOR含量均增加(F=0.037、0.026,P<0.05),Bax蛋白含量减少(F=0.018,P<0.05);rhIL-11治疗组各指标较照射组差异无统计学意义.结论 3.0 Gy中子照射可使小鼠骨髓造血功能严重受损,rhIL-11和rhG-CSF对照射后小鼠的造血功能恢复有一定改善作用,联合使用效果优于单独使用.
목적 탐토rhIL-11화rhG-CSF대중자조사치소서골수손상적치료작용.방법 장BALB/c소서130지용수궤수자표법분위대조조(30지)、조사조(40지)、rhIL-11치료조(30지)、rhIL-11+rhG-CSF치료조(30지),용3.0 Gy중자조사,병우조사후급여600 μg·kg-1·d-1적rhIL-11화50 μg·kg-1·d-1적rhG-CSF,1차/d,공3 d;용혈세포자동분석의、HE염색、AgNOR염색、류식세포술、면역조직화학염색화도상분석등,검측소서외주혈세포、골수병리형태변화、유핵세포계수、AgNOR함량、조망여배사솔화Bax단백함량적변화.결과 조사조화rhIL-11치료조우조사후6 h～3 d,소서외주혈백세포、골수유핵세포계수화AgNOR함량진행성하강,Bax단백우조혈세포포질내정양성지강양성표체,함량진행성증가(F=0.003,P<0.01);조사후6 h,조사조소서조혈세포조망여배사솔증가,이배사위현저;조사후3 d,rhIL-11+rhG-CSF치료조소서적골수유핵세포계수화AgNOR함량균증가(F=0.037、0.026,P<0.05),Bax단백함량감소(F=0.018,P<0.05);rhIL-11치료조각지표교조사조차이무통계학의의.결론 3.0 Gy중자조사가사소서골수조혈공능엄중수손,rhIL-11화rhG-CSF대조사후소서적조혈공능회복유일정개선작용,연합사용효과우우단독사용.
Objective To explore the therapeutic effect of rhIL-11 and rhG-CSF on mouse bone marrow injury induced by neutron irradiation.Methods 130 male BALB/c mice were irradiated by 3.0 Gy neutron and mice peripheral blood cells,bone marrow pathological changes,bone marrow nucleated cell counts,AgNOR content,apoptosis and necrosis rates and Bax protein content were observed by means of blood cells automatic analyzer,HE staining,AgNOR staining,flow cytometry,immunohistochemistry staining and image analysis.Results In the irradiation group and the rhIL-11 group,the mice peripheral blood white blood cells,bone marrow nucleated cell counts and AgNOR content was decreased progressively.The Bax protein was positively or strongly positively expressed in the cytoplasm of the hematopoietic cells and the Bax protein content was increased progressively at 6 h,1 d,3 d after irradiation.In the irradiation group,the rates of apoptosis and necrosis in the mice hematopoietic cells were greatly increased and that of necrosis was significant at 6 h after irradiation.In the rhIL-11 + rhG-CSF group,the counts of bone marrow nucleated cell and AgNOR were increased and the Bax protein content was decreased at 3 d after irradiation,while in the rhIL-11 group,the indexes mentioned above were not obviously different compared with those of the irradiation group.Conclusions The mice bone marrow hematopoietic function is seriously damaged by 3.0 Gy neutron irradiation,rhIL-11 and rhG-CSF could improve the mice hernatopoietic function after neutron irradiation,and combination of them is more effective to stimulate the hematopoitic function than either of them alone.