CAJ | 학술논문

目的观测Hedgehog信号通路在脑胶质瘤的表达,探讨其表达在脑胶质瘤的预后意义.方法选取118例原发性脑胶质瘤患者的手术切除标本,运用免疫组织化学方法检测Sonic hedgehog(Shh)、受体Patched(Ptch)及下游转录因子Gli1的表达,采用Kaplan-Meier生存分析和Cox比例风险回归模型评价脑胶质瘤患者的预后.结果免疫染色结果显示Shh、Ptch和Gli1的阳性表达率随胶质瘤病理等级升高呈增强趋势(P＜0.01);随KPS评分的下降而成增强趋势(P＜0.01).生存分析表明,阳性表达Shh、Ptch和Gli1的胶质瘤患者总体存活率低于三者不表达的患者(P＜0.01).多因素Cox分析显示KPS(P＜0.05)、WHO grade(P＜0.01)、Shh(P＜0.05)、Ptch (P＜0.05)和Gli1(P＜0.05)是影响脑胶质瘤预后的独立因素.结论脑胶质瘤的Shh-Ptch1-Gli1 信号通路处于激活状态,与脑胶质瘤的临床病理特征及预后参数密切相关,提示Hedgehog信号通路的活化在脑胶质瘤的恶性潜能和患者的生存时间起重要的预示作用.
목적 관측Hedgehog신호통로재뇌효질류적표체,탐토기표체재뇌효질류적예후의의.방법 선취118례원발성뇌효질류환자적수술절제표본,운용면역조직화학방법검측Sonic hedgehog(Shh)、수체Patched(Ptch)급하유전록인자Gli1적표체,채용Kaplan-Meier생존분석화Cox비례풍험회귀모형평개뇌효질류환자적예후.결과 면역염색결과현시Shh、Ptch화Gli1적양성표체솔수효질류병리등급승고정증강추세(P＜0.01);수KPS평분적하강이성증강추세(P＜0.01).생존분석표명,양성표체Shh、Ptch화Gli1적효질류환자총체존활솔저우삼자불표체적환자(P＜0.01).다인소Cox분석현시KPS(P＜0.05)、WHO grade(P＜0.01)、Shh(P＜0.05)、Ptch (P＜0.05)화Gli1(P＜0.05)시영향뇌효질류예후적독립인소.결론 뇌효질류적Shh-Ptch1-Gli1 신호통로처우격활상태,여뇌효질류적림상병리특정급예후삼수밀절상관,제시Hedgehog신호통로적활화재뇌효질류적악성잠능화환자적생존시간기중요적예시작용.
Objective Study the expression of Hedgehog signal channel in glioma, trying to find the signification of its expression to the prognosis of patient. Methods Immunohistochemical staining was done on 118 samples of glioma resection, expression of sonic hedgehog (Shh), its receptor:Patched (Ptch)and downwards transcription factor Gli1, using Kaplan-Meier survive analysis and Cox proportional hazards regression model to estimate the prognosis of patients with glioma. Results The positive expression of Shh/Ptch/Gli1 is higher as the increase of WHO grade of glioma (P ＜0. 01 ) ;is higher as thc grade of KPS decreased. Survivor analysis showed the survive rate of glioma with positive expression of Shh/Ptch/Gli1 is lower than that without expression ( P ＜0.01 ). Cox analysis showed KPS ( P ＜ 0. 05 ), WHO grade ( P ＜the prognosis of glioma. Conclusion Positive expression of Shh/Ptch/Glilis in close relation of pathological character and prognosis, the activation of Hedgehog signal channel has a important indication of the level of maglinancy and survive time of the patient.