中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2011年
6期
976-978,封4
,共4页
隐睾%生殖股神经%血管内皮细胞生长因子%内皮型一氧化氮合酶
隱睪%生殖股神經%血管內皮細胞生長因子%內皮型一氧化氮閤酶
은고%생식고신경%혈관내피세포생장인자%내피형일양화담합매
Cryptorchidism%Genitofemoral never%Vascular endothelial growth factor%Endothelial nitric oxide synthase
目的 探讨大鼠隐睾中内皮型一氧化氮合酶(eNOS)和血管内皮细胞生长因子(VEGF)的表达与单侧隐睾对侧睾丸损害的关系.方法 将45只雄性SD大鼠(22 日龄)随机分成单侧隐睾并生殖股神经离断组(A组)、单侧隐睾组(B组)和假手术组(C组),每组15只.动物模型建立后(65日龄),苏木素-伊红(HE)染色观察睾丸生精上皮形态,原位缺口末端标记法(TUNEL)检测对侧睾丸生精细胞的凋亡,免疫组织化学和Western blot方法检测对侧睾丸组织中eNOS和VEGF基因表达的变化.结果 B组相对于A组和C组对侧睾丸组织中生精细胞的凋亡率最高(t1=3.04,t2=3.94,t1,t2＞t28,P＜0.01),Western blot和免疫组织化学方法检测结果也显示B组eNOS和VEGF的表达含量较A组和C组都显著升高,差异有统计学意义(P＜0.01),而A组和C组的各指标差异均无统计学意义(P＞0.05).结论 eNOS和VEGF的高表达和生殖股神经在单侧隐睾对侧睾丸的损害中起重要作用,而切断生殖股神经可以阻断这一损害过程.
目的 探討大鼠隱睪中內皮型一氧化氮閤酶(eNOS)和血管內皮細胞生長因子(VEGF)的錶達與單側隱睪對側睪汍損害的關繫.方法 將45隻雄性SD大鼠(22 日齡)隨機分成單側隱睪併生殖股神經離斷組(A組)、單側隱睪組(B組)和假手術組(C組),每組15隻.動物模型建立後(65日齡),囌木素-伊紅(HE)染色觀察睪汍生精上皮形態,原位缺口末耑標記法(TUNEL)檢測對側睪汍生精細胞的凋亡,免疫組織化學和Western blot方法檢測對側睪汍組織中eNOS和VEGF基因錶達的變化.結果 B組相對于A組和C組對側睪汍組織中生精細胞的凋亡率最高(t1=3.04,t2=3.94,t1,t2＞t28,P＜0.01),Western blot和免疫組織化學方法檢測結果也顯示B組eNOS和VEGF的錶達含量較A組和C組都顯著升高,差異有統計學意義(P＜0.01),而A組和C組的各指標差異均無統計學意義(P＞0.05).結論 eNOS和VEGF的高錶達和生殖股神經在單側隱睪對側睪汍的損害中起重要作用,而切斷生殖股神經可以阻斷這一損害過程.
목적 탐토대서은고중내피형일양화담합매(eNOS)화혈관내피세포생장인자(VEGF)적표체여단측은고대측고환손해적관계.방법 장45지웅성SD대서(22 일령)수궤분성단측은고병생식고신경리단조(A조)、단측은고조(B조)화가수술조(C조),매조15지.동물모형건립후(65일령),소목소-이홍(HE)염색관찰고환생정상피형태,원위결구말단표기법(TUNEL)검측대측고환생정세포적조망,면역조직화학화Western blot방법검측대측고환조직중eNOS화VEGF기인표체적변화.결과 B조상대우A조화C조대측고환조직중생정세포적조망솔최고(t1=3.04,t2=3.94,t1,t2＞t28,P＜0.01),Western blot화면역조직화학방법검측결과야현시B조eNOS화VEGF적표체함량교A조화C조도현저승고,차이유통계학의의(P＜0.01),이A조화C조적각지표차이균무통계학의의(P＞0.05).결론 eNOS화VEGF적고표체화생식고신경재단측은고대측고환적손해중기중요작용,이절단생식고신경가이조단저일손해과정.
Objective To study the relationship between the expression of endothelial nitric oxide synthase (eNOS) and vascular endothelial growth factor (VEGF) with the damage of contralateral testis of unilateral cryptorchidism in the experimental rats. Methods Forty-five immature male Sprague-Dawley rats (aged 22 days) were randomly divided into the group A (unilateral cryptorchidism and the ipsilateral genitofemoral nerve division), group B (unilateral cryptorchidism), group C (sham operation), n = 15 in each group. When the rats were aged 65 days, all the rats were sacrificed and the testes were obtained. The morphological changes of the spermatogenic epithelium in the testes were observed, and the germ cell apoptosis was detected by the TdT-mediated dUTP-biotin nick end labeling (TUNEL) technique. The expression of eNOS and VEGF was detected by using Western blotting and immunohistochemistry in the testicular tissues.Results The germ cell apoptosis was increased significantly ( t1 = 3. 04, t2 = 3. 94, t1 ,t2 ＞ t28 , P ＜0. 01) , and the levels of eNOS and VEGF in the contralateral testes were also increased obviously in group B as compared with groups A and C ( P ＜ 0. 01) , but the entire indexes in groups A and C had no significant difference. Conclusion eNOS, VEGF and genitofemoral nerve play a important role in the damage of the contralateral testes of unilateral cryptorchidism, and the damage can be prevented by genitofemoral nerve division.
