背景:健脾补肾中药对实验性脾虚证有较好的防治作用,环核苷酸及甲状腺激素水平的变化是否与药物作用机制有关?目的:观察健脾补肾方药对实验性脾虚证大鼠环核苷酸及甲状腺激素水平的影响,探讨该方防治脾虚证的作用机制及脾与肾相互关系的意义.设计:随机对照的实验研究.地点和材料:实验地点为广州中医药大学核医学教研室.实验动物为雄性SD大鼠,体质量190～230 g,3月龄,普通级,由中山大学实验动物中心提供(动物合格证号为2001A032).干预:将大鼠随机分为正常对照组(n=10)、脾虚模型组(n=12),健脾补肾方高剂量组(n=12)和健脾补肾方低剂量组(n=12),采用大黄复制脾虚证动物模型,分别给予生理盐水、大黄、健脾补肾方煎液(高剂量:22 g/kg,低剂量:11g/kg)灌胃.各组动物于最后1次给药后1 h从眼球取血,观察各组大鼠血浆环磷酸腺苷(cyclic adenosine monophos-phate,cAMP),环磷酸鸟苷(cyclic guanosine monophosphate,cGMP),及甲状腺素(thyroxine,T4)、三碘甲腺原氨酸(thiiodothyronine,T3)、促甲状腺素(thyroid stimulating hormone,TSH)水平的变化.主要观察指标:各组大鼠cAMP,cGMP,T4,T3,TSH水平.结果:脾虚模型组大鼠血浆cAMP含量[(30.29±6.55)nmol/L]及cAMP/cGMP(2.94±1.13)比值均比正常对照组升高,T3[(1.55±:0.23)nmol/L],T4[(67.73±14.17)nmol/L]含量比正常对照组下降,差异有显著性意义(q=5.87,7.91,5.61,P均＜0.01).高剂量健脾补肾方药能降低实验性脾虚证大鼠体内cAMP含量[(20.81±3.75)nmol/L]及cAMP/cGMP比值(1.34±0.52),提高T3[(2.19±0.38)nmol/L],T4[(89.70±21.27)nmol/L]含量,与脾虚模型组比较,差异有显著性意义(q=6.49,6.56,7.86,4.87,P均＜0.01);升高脾脏、胸腺质量比值,减轻肾脏组织质量.结论:健脾补肾中药可以消除大黄的药物影响,防止出现脾虚症候,其作用机制与调节cAMP、cGMP及甲状腺激素水平有关.
揹景:健脾補腎中藥對實驗性脾虛證有較好的防治作用,環覈苷痠及甲狀腺激素水平的變化是否與藥物作用機製有關?目的:觀察健脾補腎方藥對實驗性脾虛證大鼠環覈苷痠及甲狀腺激素水平的影響,探討該方防治脾虛證的作用機製及脾與腎相互關繫的意義.設計:隨機對照的實驗研究.地點和材料:實驗地點為廣州中醫藥大學覈醫學教研室.實驗動物為雄性SD大鼠,體質量190～230 g,3月齡,普通級,由中山大學實驗動物中心提供(動物閤格證號為2001A032).榦預:將大鼠隨機分為正常對照組(n=10)、脾虛模型組(n=12),健脾補腎方高劑量組(n=12)和健脾補腎方低劑量組(n=12),採用大黃複製脾虛證動物模型,分彆給予生理鹽水、大黃、健脾補腎方煎液(高劑量:22 g/kg,低劑量:11g/kg)灌胃.各組動物于最後1次給藥後1 h從眼毬取血,觀察各組大鼠血漿環燐痠腺苷(cyclic adenosine monophos-phate,cAMP),環燐痠鳥苷(cyclic guanosine monophosphate,cGMP),及甲狀腺素(thyroxine,T4)、三碘甲腺原氨痠(thiiodothyronine,T3)、促甲狀腺素(thyroid stimulating hormone,TSH)水平的變化.主要觀察指標:各組大鼠cAMP,cGMP,T4,T3,TSH水平.結果:脾虛模型組大鼠血漿cAMP含量[(30.29±6.55)nmol/L]及cAMP/cGMP(2.94±1.13)比值均比正常對照組升高,T3[(1.55±:0.23)nmol/L],T4[(67.73±14.17)nmol/L]含量比正常對照組下降,差異有顯著性意義(q=5.87,7.91,5.61,P均＜0.01).高劑量健脾補腎方藥能降低實驗性脾虛證大鼠體內cAMP含量[(20.81±3.75)nmol/L]及cAMP/cGMP比值(1.34±0.52),提高T3[(2.19±0.38)nmol/L],T4[(89.70±21.27)nmol/L]含量,與脾虛模型組比較,差異有顯著性意義(q=6.49,6.56,7.86,4.87,P均＜0.01);升高脾髒、胸腺質量比值,減輕腎髒組織質量.結論:健脾補腎中藥可以消除大黃的藥物影響,防止齣現脾虛癥候,其作用機製與調節cAMP、cGMP及甲狀腺激素水平有關.
배경:건비보신중약대실험성비허증유교호적방치작용,배핵감산급갑상선격소수평적변화시부여약물작용궤제유관?목적:관찰건비보신방약대실험성비허증대서배핵감산급갑상선격소수평적영향,탐토해방방치비허증적작용궤제급비여신상호관계적의의.설계:수궤대조적실험연구.지점화재료:실험지점위엄주중의약대학핵의학교연실.실험동물위웅성SD대서,체질량190～230 g,3월령,보통급,유중산대학실험동물중심제공(동물합격증호위2001A032).간예:장대서수궤분위정상대조조(n=10)、비허모형조(n=12),건비보신방고제량조(n=12)화건비보신방저제량조(n=12),채용대황복제비허증동물모형,분별급여생리염수、대황、건비보신방전액(고제량:22 g/kg,저제량:11g/kg)관위.각조동물우최후1차급약후1 h종안구취혈,관찰각조대서혈장배린산선감(cyclic adenosine monophos-phate,cAMP),배린산조감(cyclic guanosine monophosphate,cGMP),급갑상선소(thyroxine,T4)、삼전갑선원안산(thiiodothyronine,T3)、촉갑상선소(thyroid stimulating hormone,TSH)수평적변화.주요관찰지표:각조대서cAMP,cGMP,T4,T3,TSH수평.결과:비허모형조대서혈장cAMP함량[(30.29±6.55)nmol/L]급cAMP/cGMP(2.94±1.13)비치균비정상대조조승고,T3[(1.55±:0.23)nmol/L],T4[(67.73±14.17)nmol/L]함량비정상대조조하강,차이유현저성의의(q=5.87,7.91,5.61,P균＜0.01).고제량건비보신방약능강저실험성비허증대서체내cAMP함량[(20.81±3.75)nmol/L]급cAMP/cGMP비치(1.34±0.52),제고T3[(2.19±0.38)nmol/L],T4[(89.70±21.27)nmol/L]함량,여비허모형조비교,차이유현저성의의(q=6.49,6.56,7.86,4.87,P균＜0.01);승고비장、흉선질량비치,감경신장조직질량.결론:건비보신중약가이소제대황적약물영향,방지출현비허증후,기작용궤제여조절cAMP、cGMP급갑상선격소수평유관.
BACKGROUND: Although jianpi bushen recipe(JPBS) acted satisfactorily on the treatment of experimental spleen deficiency, whether did the changes in levels of cyclic nucleotide and thyroid hormone be related to the mechanism of the recipe?OBJECTIVE: To observe the effect of JPBS on the level of cyclic adenosine monophosphate (cAMP), cyclic guanosine monophosphate (cGMP) and the thyroid hormone in rats with experimental spleen deficiency and probe into the mechanism of the recipe on the treatment, as well as the interrelationship between spleen and kidney.DESIGN: Randomized controlled experiment.SETTING and MATERIALS: The experiment was performed in the Department of Nucleus Medical of Guangzhou University of Traditional Chinese Medicine. Male SD rats, weighing 190 to 230 g, at the age of 3 months, nor mai class, were employed in the experiment, which were provided by the Experimental Animal Center of Sun Yat-sen University(Qualified No. 2001A032).INTERVENTIONS: The rats were divided at random into normal control group( n = 10), model group of spleen deficiency( n = 12), JPBS high dosage group( n = 12) and JPBS low dosage group( n = 12) . The model of spleendeficiency was duplicated with dahuan. Gastric infusion was done with physiological saline, dahuan and JPBS decoction(high dosage: 22 g/kg, low dosage: 11 g/kg). Blood was collected from eyeball at the last medication in every group to observe the changes in levels of cAMP, cyclic cGMP, thyroxine(T4), thiiodothyronine(T3), and thyroid stimulating hormone(TSH) of rats in every group.MAIN OUTCOME MEASURES: Levels of cAMP, cGMP, T4, T3 and TSH of rats in every group.RESULTS: In model group of spleen deficiency, the content of serum cAMP [ (30. 29±6.55)nmol/L] and cAMP/cGMP(2.94±1.13)were higher than the normal control group; the contents of T3[(1.55±0.23 nmol/L) and T4[ (67.73 ± 14. 17 nmol/L)were reduced compared with the normal control,indicating significant difference( q = 5.87, 7.91, 5.61, P ＜ 0.01) . JPBS recipe of high dosage reduced the content of cAMP[ (20. 81 ± 3.75 nmol/L)and cAMP/cGMP was(1.34±0.52) and increased the contents of T3[ (2.19±0.38 nmol/L) and T4 [ (89.70 ± 21.27) nmol/L] in the rats with experimental spleen deficiency, compared with the model of spleen deficiency, indicating significant difference( q = 6.49, 6.56, 7.86,4. 87; P ＜0.01) and it also increased the mass ratio of spleen and thymus and decreased the mass of kidney tissues.CONCLUSION: JPBS recipe acted on reducing the medical influence of dahuan and preventiong the symatoms of spleen deficiency.The mechanism of it was related to the levels of cAMP ,cGMP and thyroid hormone.
