华中师范大学学报（自然科学版）
華中師範大學學報（自然科學版）
화중사범대학학보（자연과학판）
JOURNAL OF CENTRAL CHINA NORMAL UNIVERSITY(NATURAL SCIENCES)
2009年
4期
633-636
,共4页
新疆雪莲%愈伤组织%植物激素
新疆雪蓮%愈傷組織%植物激素
신강설련%유상조직%식물격소
Saussurea involucrate%callus%plant hormones
研究了不同植物激素对新疆雪莲叶片愈伤组织诱导和分化的影响.结果表明:植物激素对新疆雪莲愈伤组织的诱导和分化影响显著,以MS为基本培养基附加浓度1.OO mg·L~(-1)的6-BA和2.OO mg·L~(-1)的NAA组合为最佳诱导培养基,诱导率达100%;以MS为基本培养基附加浓度1.00 mg·L~(-1)的6-BA和O.20 mg·L~(-1)的NAA组合为最值出芽培养基,出芽率达78%;以1/2MS为基本培养基附加浓度O.30 mg·L~(-1)的NAA和浓度O.03 mg·L~(-1)的KT为最佳生根培养基,生根率达54%.
研究瞭不同植物激素對新疆雪蓮葉片愈傷組織誘導和分化的影響.結果錶明:植物激素對新疆雪蓮愈傷組織的誘導和分化影響顯著,以MS為基本培養基附加濃度1.OO mg·L~(-1)的6-BA和2.OO mg·L~(-1)的NAA組閤為最佳誘導培養基,誘導率達100%;以MS為基本培養基附加濃度1.00 mg·L~(-1)的6-BA和O.20 mg·L~(-1)的NAA組閤為最值齣芽培養基,齣芽率達78%;以1/2MS為基本培養基附加濃度O.30 mg·L~(-1)的NAA和濃度O.03 mg·L~(-1)的KT為最佳生根培養基,生根率達54%.
연구료불동식물격소대신강설련협편유상조직유도화분화적영향.결과표명:식물격소대신강설련유상조직적유도화분화영향현저,이MS위기본배양기부가농도1.OO mg·L~(-1)적6-BA화2.OO mg·L~(-1)적NAA조합위최가유도배양기,유도솔체100%;이MS위기본배양기부가농도1.00 mg·L~(-1)적6-BA화O.20 mg·L~(-1)적NAA조합위최치출아배양기,출아솔체78%;이1/2MS위기본배양기부가농도O.30 mg·L~(-1)적NAA화농도O.03 mg·L~(-1)적KT위최가생근배양기,생근솔체54%.
Using Saussurea involucrate leaves, the effect of callus induction and differentiation on MS medium with different plant hormones was studied on this paper. The results showed that callus induction and differentiation of leaves with different plant hormones have significantly statistical differences. The medium containing 1, 00 mg · L~(-1) 6-BA and 2. 00 mg · L~(-1) NAA was the suitable medium for callus induction, the frequency of callus induction was 100% ; The medium containing 1. 00 mg · L~(-1) 6-BA and 0. 20 mg · L~(-1) NAA was the suitable medium for differentiation, the frequency of callus differentiation was 78%; Then the regenerated plants were rooted on 1/2MS medium containing 0. 03 mg · L~(-1) 6-BA and 0. 30 mg · L~(-1) NAA and the rooting rate was 54%.
