CAJ | 학술논문

通过DAPI荧光计数、恢复培养和变性梯度凝胶电泳,分析了玉龙雪山不同深度积雪中的细菌数量、多样性及其群落结构.结果显示:玉龙雪山雪坑样品中细菌数量和多样性指数与气候环境替代指标W(Ca~(2+)),W(Mg~(2+)),W(Cl~-)等具有相关性.可培养细菌隶属于三个不同系统发育群:Actinobactema(37%),Firmicutes(11%)和Proteobacteria(α-Proteobacteria)(52%),其中α-Proteobacteria类为优势类群.DGGE测序结果表明细菌属β-Proteobacteria,Actinobacteria和Bacteroidetes类群,以β-Proteobacteria为优势类群,并且不同深度雪坑中细菌多样性和群落结构没有明显变化.结果表明:海洋型冰川雪中细菌数量和多样性、群落结构与大陆型冰川雪相比,冰雪微生物具有其特殊性.
통과DAPI형광계수、회복배양화변성제도응효전영,분석료옥룡설산불동심도적설중적세균수량、다양성급기군락결구.결과현시:옥룡설산설갱양품중세균수량화다양성지수여기후배경체대지표W(Ca~(2+)),W(Mg~(2+)),W(Cl~-)등구유상관성.가배양세균대속우삼개불동계통발육군:Actinobactema(37%),Firmicutes(11%)화Proteobacteria(α-Proteobacteria)(52%),기중α-Proteobacteria류위우세류군.DGGE측서결과표명세균속β-Proteobacteria,Actinobacteria화Bacteroidetes류군,이β-Proteobacteria위우세류군,병차불동심도설갱중세균다양성화군락결구몰유명현변화.결과표명:해양형빙천설중세균수량화다양성、군락결구여대륙형빙천설상비,빙설미생물구유기특수성.
The total bacterial counts, bacterial diversity and community structure in mountain snow pits from the Mt. Yulong regions, China, were analyzed with 4,6-diamino-2-phenylindole (DAPI), culturedependent and culture-independent methods, denaturing gradient gel electrophoresis (DGGE). The microbial abundance and diversity index have a close relationship with mineral particle concentration. These mineral particles, such as W(Ca~(2+)), W(Mg~(2+)) and W(Cl~-) etc, are good indicators of climate and environment. The 16S rRNA genes of cultured bacteria belong to following groups: Actinobacteria (37%), Firmicutes (11%),Proteobacteria (α-Proteobacteria) (52%). Among these, α-Proteobacteria are dominant groups. DGGE band sequenced belongs to following groups:β- Proteobacteria, Actinobacteria and Bacteroidetes. β-Proteobacteria are dominant groups. The bacterial diversity and community structures do not change obviously in different depth snow samples. Comparing the microbial abundance, diversity and community structures in snow of inland glacier and maritime glacier, the microorganism of maritime glacier has its particularity.