中国中西医结合杂志
中國中西醫結閤雜誌
중국중서의결합잡지
CHINESE JOURNAL OF INTEGRATED TRADITIONAL AND WESTERN MEDICINE
2010年
2期
182-187
,共6页
唐巍%王键%王又闻%倪朝民%陈业农%唐照亮%管叶明%俞丽华%李晓民%胡建鹏
唐巍%王鍵%王又聞%倪朝民%陳業農%唐照亮%管葉明%俞麗華%李曉民%鬍建鵬
당외%왕건%왕우문%예조민%진업농%당조량%관협명%유려화%리효민%호건붕
神经干细胞%药物血清%增殖%脑络欣通%左归丸
神經榦細胞%藥物血清%增殖%腦絡訢通%左歸汍
신경간세포%약물혈청%증식%뇌락흔통%좌귀환
neural stem cells%drug contained serum%proliferation%Naoluo Xintong%Zuogui Pill
目的 探讨脑络欣通药物血清和左归丸药物血清对体外培养的大鼠胚胎神经干细胞(neural stem cell,NSC)增殖分化的作用.方法 分别采用含10%脑络欣通药物血清和含10%左归丸药物血清的培养基培养大鼠胚胎NSC,观察其促增殖效应和诱导分化效应,应用相差显微镜和免疫荧光染色对其进行比较观察.结果 脑络欣通药物血清和左归丸药物血清均可诱导绝大多数NSC分化成神经元、星形胶质细胞和少突胶质细胞.此外,两者还能在一定程度上促进培养的NSC生长.前者诱导NSC分化的进程虽比后者要慢,但诱导NSC向神经元方向分化的比率较高,其分化的神经元在细胞形态学上与发育成熟的神经元更为接近;后者促进NSC生长的效应较为显著.结论 脑络欣通和左归丸均能促进体外培养的NSC生长和分化,两者作用有一定差异;益气活血治法和补肾生髓治法对NSC进行诱导分化具有一定的可行性.
目的 探討腦絡訢通藥物血清和左歸汍藥物血清對體外培養的大鼠胚胎神經榦細胞(neural stem cell,NSC)增殖分化的作用.方法 分彆採用含10%腦絡訢通藥物血清和含10%左歸汍藥物血清的培養基培養大鼠胚胎NSC,觀察其促增殖效應和誘導分化效應,應用相差顯微鏡和免疫熒光染色對其進行比較觀察.結果 腦絡訢通藥物血清和左歸汍藥物血清均可誘導絕大多數NSC分化成神經元、星形膠質細胞和少突膠質細胞.此外,兩者還能在一定程度上促進培養的NSC生長.前者誘導NSC分化的進程雖比後者要慢,但誘導NSC嚮神經元方嚮分化的比率較高,其分化的神經元在細胞形態學上與髮育成熟的神經元更為接近;後者促進NSC生長的效應較為顯著.結論 腦絡訢通和左歸汍均能促進體外培養的NSC生長和分化,兩者作用有一定差異;益氣活血治法和補腎生髓治法對NSC進行誘導分化具有一定的可行性.
목적 탐토뇌락흔통약물혈청화좌귀환약물혈청대체외배양적대서배태신경간세포(neural stem cell,NSC)증식분화적작용.방법 분별채용함10%뇌락흔통약물혈청화함10%좌귀환약물혈청적배양기배양대서배태NSC,관찰기촉증식효응화유도분화효응,응용상차현미경화면역형광염색대기진행비교관찰.결과 뇌락흔통약물혈청화좌귀환약물혈청균가유도절대다수NSC분화성신경원、성형효질세포화소돌효질세포.차외,량자환능재일정정도상촉진배양적NSC생장.전자유도NSC분화적진정수비후자요만,단유도NSC향신경원방향분화적비솔교고,기분화적신경원재세포형태학상여발육성숙적신경원경위접근;후자촉진NSC생장적효응교위현저.결론 뇌락흔통화좌귀환균능촉진체외배양적NSC생장화분화,량자작용유일정차이;익기활혈치법화보신생수치법대NSC진행유도분화구유일정적가행성.
Objective To investigate the effects of drug-contained sera (DCS) of Naoluo Xintong (NLXT) and Zuogui Pill (ZGP) on the proliferation and differentiation of in vitro cultured embryonic neural stem cells (NSCs) in rats. Methods Rat's embryonic NSCs were cultured in medium supplemented with 10% DCS with NLXT and ZGP separately,the effects of DCS in enhancing proliferation and inducing differentiation were observed and compared by phase contrast microscopy and immunofluorescence staining. Results Majority of NSCs were induced to neurons,astrocytes or oligodendrocytes in medium supplemented with either DCS-NLXT or DCS-ZGP,with the growth of them promoted to some extent. However,DCS-NLXT induced the differentiation rather slowly but with the differentiated neurons more resemble to the mature neuron in morphology,while DCS-ZGP promoted the stem cell growth more effectively. Conclusion Both NLXT and ZGP could promote the differentiation and growth of in vitro cultured NSCs,but with exiguous difference. It is feasible to induce the proliferation and differentiation of NSCs by way of using Chinese medicine drug-therapy for supplementing qi and activating blood circulation as well as that for tonifying Shen to generate marrow.
