CAJ | 학술논문

目的观察棕桐酸盐对胰岛β细胞凋亡及胰腺衍生因子(PANDER)的影响,并观察胰升血糖素样肽1(GLP-1)对其的干预.方法培养β-TC3细胞分别以PA、PA+GLP-1以及空白处理24h,以MTT法、Annexin-V/PI荧光染色流式细胞术、Tunel荧光染色法检测细胞凋亡,以RT-PCR法测定PANDER mRNA的表达.结果 PA浓度达0.25mmol/L时细胞存活率开始显著下降,并随浓度上升逐渐下降(P＜0.05).GLp-1能显著拮抗PA致胰岛β细胞凋亡;PA能明显增加PANDER mRNA的水平,而GLp-1能显著抑制PA对PANDER的作用.结论 PA在诱导胰岛β细胞凋亡的同时刺激PANDER表达,GLP-1拮抗PA致胰岛β细胞凋亡及PANDER的表达;PANDER可能是GLP-1拮抗胰岛β细胞脂性凋亡的靶点之一.
목적 관찰종동산염대이도β세포조망급이선연생인자(PANDER)적영향,병관찰이승혈당소양태1(GLP-1)대기적간예.방법 배양β-TC3세포분별이PA、PA+GLP-1이급공백처리24h,이MTT법、Annexin-V/PI형광염색류식세포술、Tunel형광염색법검측세포조망,이RT-PCR법측정PANDER mRNA적표체.결과 PA농도체0.25mmol/L시세포존활솔개시현저하강,병수농도상승축점하강(P＜0.05).GLp-1능현저길항PA치이도β세포조망;PA능명현증가PANDER mRNA적수평,이GLp-1능현저억제PA대PANDER적작용.결론 PA재유도이도β세포조망적동시자격PANDER표체,GLP-1길항PA치이도β세포조망급PANDER적표체;PANDER가능시GLP-1길항이도β세포지성조망적파점지일.
Objective To observe the effects of palmitate (PA) on the pancreatic β-cell apoptosis and PANDER mRNA expression, and the effects of GLP-1 on fatty acid-induced pancreatic β-cell apoptosis and PANDER expression. Methods β-TC3 cells were cultured with vehicle (control), 0.5 mmol/L PA, 0.5 mmol/L PA + 10 nmol/L GLP-1, respectively for 24 hours. Cell apoptosis were evaluated with MTT, Annexin-V-FITC/PI FACS and Tunel assay. PANDER mRNA expression were measured by RT-PCR. Results MTT analysis showed that PA inhibited β-TC3 cell viability in a dose dependent manner with the significantly viability decrease beginning at 0.25 mmol/L PA.GLP-1 counteracted the apoptotic effects of PA on β-TC3 cell. PANDER mRNA increased significantly in PA treated cells (vs control，P<0.05), while GLP-1 inhibited PA induced PANDER mRNA over expression. Conclusion FFA induces pancreatic β-cells apoptosis and PANDER over expression, GLP-1 counteracts the effects of FFA on pancreatic β-cell and PANDER expression. PANDER may be an important mediator in the signaling pathway of lipoapoptosis of pancreatic beta-cell.