CAJ | 학술논문

目的通过对食管癌免疫功能与微转移关系的研究,了解免疫功能在食管癌微转移中的作用与地位.方法用流式细胞仪测定外周血T淋巴细胞亚群CD3+、CD4+、CD8+、CD4+/CD8+比值,选取食管胸中段鳞癌常规病理检查未发现癌细胞转移者淋巴结,免疫组织化学方法检测微转移,将无微转移和有微转移分为两组,对其T淋巴细胞亚群水平进行对比.结果有微转移组CD3+、CD4+水平及CD4+/CD8+比值明显低于无微转移组,CD8+水平明显高于无微转移组,差异均有统计学意义(均P<0.05).结论 T淋巴细胞亚群与微转移密切相关,低水平CD3+、CD4+和高水平CD8+提示预后较差.
목적 통과대식관암면역공능여미전이관계적연구,료해면역공능재식관암미전이중적작용여지위.방법 용류식세포의측정외주혈T림파세포아군CD3+、CD4+、CD8+、CD4+/CD8+비치,선취식관흉중단린암상규병리검사미발현암세포전이자림파결,면역조직화학방법 검측미전이,장무미전이화유미전이분위량조,대기T림파세포아군수평진행대비.결과 유미전이조CD3+、CD4+수평급CD4+/CD8+비치명현저우무미전이조,CD8+수평명현고우무미전이조,차이균유통계학의의(균P<0.05).결론 T림파세포아군여미전이밀절상관,저수평CD3+、CD4+화고수평CD8+제시예후교차.
Objective To acknowledge the effect of T-lymphocyte subset on lymph node micrometastasis in esophageal cancer patients by the study of the correlation between T-lymphocyte subset and micrometastasis.Methods CD3+、CD4+、CD8+and CD4+/CD8+ were examined in esophageal cancer patients under thoracotomy by flow cytometry,and the micrometastasis were examined by immunohistochemistry in lymph node that were diagnosed as negative for metastases by routine histopathological analysis.Two groups were divided by with or without micro-metastasis,T-lymphocyte subset were compared with two groups.Results CD3+,CD4+ and CD4+/CD8+were significantly lower in group with micrometastasis than in group without micrometastasis,CD8+ was significantly higher in group with micrometagtasis than in group without micrometastasis.Conclusion T-lymphocyte subset is closely associmed with micrometastasis and low CD3+,CD4+ and high CD8+ T-lymphocyte is associated with poor cancer-specific survival.