CAJ | 학술논문

目的研究慢性应激抑郁模型大鼠海马cAMP反应元件结合蛋白(CREB)的表达情况及氟西汀的干预作用.方法将大鼠随机分为抑郁模型组、氟西汀治疗组和对照组.模型组和氟西汀组给予21d的应激刺激,此期间对照组正常饲养,刺激期间氟西汀组每天腹腔注射氟西汀(10mg/kg),模型组和对照组每天腹腔注射等体积的生理盐水.行为学检测应用open-field法和液体消耗实验.采用免疫组织化学法和Western-blotting法检测各组大鼠海马CREB的表达情况.结果应激后,模型组水平穿越格数[(8.2 ±2.7)格、竖立次数(8.1±3.5)次、修饰次数(4.3±1.6)次]、糖水消耗百分比[(52.5 ±7.8)%]均显著低于对照组[分别为(31.3±5.8)%、(13.9 ±3.2)%、(10.6 ±2.4)%、(68.3 ±4.5)%;均P＜0.01].应激后氟西汀组水平穿越格数[(15.3±7.7)格]低于对照组(P＜0.01),但高于模型组(P＜0.05);竖立次数[(8.2±5.6)次]低于对照组(P＜0.01),与模型组差异无显著性(P＞0.05);修饰次数[(6.2 ±1.5)次]低于对照组(P＜0.01),但高于模型组(P＜0.05);糖水消耗百分比(66.7 ±5.1)%与对照组差异无显著性(P＞0.05),但高于模型组(P＜0.05).免疫组织化学检测和Western-blotting法检测中,模型组大鼠海马CREB的表达均显著低于对照组(P＜0.01);氟西汀组大鼠海马CREB的表达均显著低于对照组(P＜0.01),但高于模型组大鼠(P＜0.01).结论慢性应激抑郁模型大鼠海马中CREB的表达降低并可以被氟西汀部分逆转.
목적 연구만성응격억욱모형대서해마cAMP반응원건결합단백(CREB)적표체정황급불서정적간예작용.방법 장대서수궤분위억욱모형조、불서정치료조화대조조.모형조화불서정조급여21d적응격자격,차기간대조조정상사양,자격기간불서정조매천복강주사불서정(10mg/kg),모형조화대조조매천복강주사등체적적생리염수.행위학검측응용open-field법화액체소모실험.채용면역조직화학법화Western-blotting법검측각조대서해마CREB적표체정황.결과 응격후,모형조수평천월격수[(8.2 ±2.7)격、수립차수(8.1±3.5)차、수식차수(4.3±1.6)차]、당수소모백분비[(52.5 ±7.8)%]균현저저우대조조[분별위(31.3±5.8)%、(13.9 ±3.2)%、(10.6 ±2.4)%、(68.3 ±4.5)%;균P＜0.01].응격후불서정조수평천월격수[(15.3±7.7)격]저우대조조(P＜0.01),단고우모형조(P＜0.05);수립차수[(8.2±5.6)차]저우대조조(P＜0.01),여모형조차이무현저성(P＞0.05);수식차수[(6.2 ±1.5)차]저우대조조(P＜0.01),단고우모형조(P＜0.05);당수소모백분비(66.7 ±5.1)%여대조조차이무현저성(P＞0.05),단고우모형조(P＜0.05).면역조직화학검측화Western-blotting법검측중,모형조대서해마CREB적표체균현저저우대조조(P＜0.01);불서정조대서해마CREB적표체균현저저우대조조(P＜0.01),단고우모형조대서(P＜0.01).결론 만성응격억욱모형대서해마중CREB적표체강저병가이피불서정부분역전.
Objective To research the expression of CREB in hippocampus of chronic stress depression rat and the interference of fluoxetine to the CREB. Methods All the experimental rats were divided by random into 3 groups:depression group, fluoxetine group and control group. The rats of depression group and fluoxetine group were applied stress for 21 days, and meanwhile the rats of control group were fed normally. The rats of fluoxetine group were applied fluoxetine by peritoneal injection(10mg/kg), and the rats of another groups were given normal sodium of the same volume by peritoneal injection. The ethology examination was performed by using method of open-field and experiment of fluid consumption. The expression of CREB was detected by immunohistochemical method and Western-blotting. Results After the chronic stress, the horizontal crossing numbers, the erection times, the modification times and the percentage of sacchar-consumption of the rats of depression group were 8. 2 ±2. 7;8. 1 4-3. 5;4. 3 4-1. 6 and 52. 5%4-7. 8%respectively, which were less than control group(31. 3 4-5. 8;13. 9 ±3. 2;10. 6 ±2. 4 and 68. 3%±4. 5%;P＜0. 01). The horizontal crossing numbers(15. 3 ±7. 7)and themodification times(6. 2±1. 5)of fluoxetine group were less than those of control group(P＜0. 01), but more than depression group(P＜0. 05);the erection times(8. 2 ±5. 6), fluoxetine group was less than control group(P＜0. 01), but no obvious difference with depression group(P＞0. 05);the percentage of sacchar-consumption (66. 7%±5. 1%), fluoxetine group was more than depression group(P＜0. 05), but no significant difference compared with control group(P＞0. 05). In both immunohistochemical method and western-blotting, the level of CREB in the hippocampus of depression group was less than that of Control group(P＜0. 01), and the level of CREB in the hippocampus of fluoxetine group was less than that of Control group(P＜0. 01)but more than that of depression group(P＜0. 01). Conclusion The expression of CREB in hippocampus of chronic stress depression rat decreased and can be reversed by fluoxetine to some extent.