CAJ | 학술논문

目的探讨脉冲电刺激对大鼠脊髓损伤后自体骨髓间充质干细胞( mesenchymal stem cells,MSCs)移植疗效的影响. 方法选用60只雄性SD大鼠.数控脊髓损伤打击器制备脊髓损伤模型.脊髓损伤7d后,从大鼠自身股骨上采集并标记MSCs.以随机数字表法分为四组:MSCs移植组(A组)、电刺激组(B组)、电刺激联合MSCs移植组(C组)和PBS对照组(D组).应用BBB评分法和体感诱发电位(SEP)在脊髓损伤前后定期进行功能评定；磁共振弥散张量成像(DTI)技术检测脊髓的修复情况；免疫组化观察Brdu标记的移植MSCs的存活情况. 结果术后4周,BBB评分C组与其他三组比较差异有统计学意义(P＜0.05),明显高于D组(P＜0.01),提示肢体功能恢复显著.移植后第8周,SEP检测C组潜伏期及波幅与其他各组比较差异有统计学意义(P＜0.05),提示C组神经功能恢复较快,DTI成像在移植后第8周也有明显修复.免疫组化显示术后8周C组脊髓损伤部位有大量Brdu标记的MSCs细胞,其阳性细胞数量和比率明显增加. 结论电刺激疗法能够显著提高脊髓损伤后移植MSCs的存活能力及其联合治疗效果.
목적 탐토맥충전자격대대서척수손상후자체골수간충질간세포( mesenchymal stem cells,MSCs)이식료효적영향. 방법 선용60지웅성SD대서.수공척수손상타격기제비척수손상모형.척수손상7d후,종대서자신고골상채집병표기MSCs.이수궤수자표법분위사조:MSCs이식조(A조)、전자격조(B조)、전자격연합MSCs이식조(C조)화PBS대조조(D조).응용BBB평분법화체감유발전위(SEP)재척수손상전후정기진행공능평정；자공진미산장량성상(DTI)기술검측척수적수복정황；면역조화관찰Brdu표기적이식MSCs적존활정황. 결과 술후4주,BBB평분C조여기타삼조비교차이유통계학의의(P＜0.05),명현고우D조(P＜0.01),제시지체공능회복현저.이식후제8주,SEP검측C조잠복기급파폭여기타각조비교차이유통계학의의(P＜0.05),제시C조신경공능회복교쾌,DTI성상재이식후제8주야유명현수복.면역조화현시술후8주C조척수손상부위유대량Brdu표기적MSCs세포,기양성세포수량화비솔명현증가. 결론 전자격요법능구현저제고척수손상후이식MSCs적존활능력급기연합치료효과.
Objective To investigate whether the implanted spike wave electrical stimulation (ES) can improve survival of mesenchymal stem cells (MSCs) after transplantation in spinal cord injury (SCI) rats. Methods In the study,60 male SD rats were used to prepare the SCI induced by a digital weight drop apparatus.The MSCs from each rat' s femurs were collected and then labeled by Bromodeoxyuridine (Brdu) seven days after SCI.The rats were randomly assigned into four groups,ie,group A (receiving a transplantation of MSCs only),group B (receiving electro-stimulant therapy only),group C ( receiving a transplation of MSCs and electro-stimulant therapy) and the control group ( receiving a single injection of phosphate buffer solution,PBS).Functional status was assessed regularly before and after SCI by using the Basso-Beattie-Bresnahan (BBB) locomotor rating score and somatosensory evoked potential (SEP).The recovery of spinal cord was checked by diffusion tensor imaging (DTI) and the survivorship of the labeled MSCs was observed with immunohistochemical method. Results BBB score of group C demonstrated significant difference in comparison with that of the other three groups ( P ＜ 0.05 )and was significantly higher than that of the control group (P ＜0.01 ) four weeks after surgery,which indicated an obvious functional improvement of the extremity mobility in group C.At the 8th week postoperatively,SEP study displayed significant difference in group C from the other three groups in terms of latency and wave amplitude (P ＜0.05 ),indicating the fast neurofunctional recovery of the spinal cord in group C.MR images of the spinal cords by DTI showed the same outcome.Immunohistochemistry confirmed a large number of the labeled MSCs positive cells in the lesion site of group C. Conclusion Theimplanted spike wave ES promotes the bioactivity and survival of MSCs and improves the therapeutic effect in combination with MSCs transplantation.