中华急诊医学杂志
中華急診醫學雜誌
중화급진의학잡지
CHINESE JOURNAL OF EMERGENCY MEDICINE
2011年
1期
74-77
,共4页
俎德玲%诸葛毅%蒋一鸣%屠晓鸣%周建英%金奇志%程科云%陆国华%郑家胜%杨庆业%高珍艳
俎德玲%諸葛毅%蔣一鳴%屠曉鳴%週建英%金奇誌%程科雲%陸國華%鄭傢勝%楊慶業%高珍豔
조덕령%제갈의%장일명%도효명%주건영%금기지%정과운%륙국화%정가성%양경업%고진염
急性心肌梗死%流式细胞术%血小板α颗粒糖蛋白CD62P%血小板超微结构
急性心肌梗死%流式細胞術%血小闆α顆粒糖蛋白CD62P%血小闆超微結構
급성심기경사%류식세포술%혈소판α과립당단백CD62P%혈소판초미결구
Acute myocardial infarction%Flow cytometry%Platelet activation-dependent granule membrane protein%Ultra microstructure change of platelets
目的 探讨急性ST段抬高心肌梗死(STEMI)患者血小板α颗粒糖蛋白(platelet activationdependent granule membrane protein,PADGEM or GMP-140,CD62P)、血小板源生长因子受体αβ亚型(platelet-derived growth factor receptor-αβ,PDGFR-αβ)表达,血小板超微结构的变化.方法 采用流式细胞仪测定36例急性ST段抬高心肌梗死患者和同期34名健康体检者(对照组)外周血中血小板的血小板a颗粒糖蛋白(CD62P)、血小板源生长因子受体αβ亚型(PDGFR-αβ)表达,采用扫描电子显微镜观察血小板超微结构,并进行比较.结果 急性STEMI组治疗前血小板α颗粒糖蛋白、血小板源生长因子受体αβ表达率分别为(3.65±1.87)%,(0.43±0.39)%,治疗后为(0.96±0.79)%,(0.28±0.24)%,对照组相应为(0.67±0.35)%,(0.27±0.22)%.STEMI组的血小板α颗粒糖蛋白、血小板源生长因子受体αβ表达率,治疗前较对照组高(分别为P＜0.01和P＜0.05),治疗后明显降低.STEMI组扫描电镜观察血小板表而超微结构变化明显.结论 STEMI血小板处于活化状态,血小板活化指标血小板α颗粒糖蛋白可作为冠心病病情监测的有效指标,血小板源生长因子受体αβ有参考价值,扫描电镜观察可发现血小板活化的超微病理结构改变.
目的 探討急性ST段抬高心肌梗死(STEMI)患者血小闆α顆粒糖蛋白(platelet activationdependent granule membrane protein,PADGEM or GMP-140,CD62P)、血小闆源生長因子受體αβ亞型(platelet-derived growth factor receptor-αβ,PDGFR-αβ)錶達,血小闆超微結構的變化.方法 採用流式細胞儀測定36例急性ST段抬高心肌梗死患者和同期34名健康體檢者(對照組)外週血中血小闆的血小闆a顆粒糖蛋白(CD62P)、血小闆源生長因子受體αβ亞型(PDGFR-αβ)錶達,採用掃描電子顯微鏡觀察血小闆超微結構,併進行比較.結果 急性STEMI組治療前血小闆α顆粒糖蛋白、血小闆源生長因子受體αβ錶達率分彆為(3.65±1.87)%,(0.43±0.39)%,治療後為(0.96±0.79)%,(0.28±0.24)%,對照組相應為(0.67±0.35)%,(0.27±0.22)%.STEMI組的血小闆α顆粒糖蛋白、血小闆源生長因子受體αβ錶達率,治療前較對照組高(分彆為P＜0.01和P＜0.05),治療後明顯降低.STEMI組掃描電鏡觀察血小闆錶而超微結構變化明顯.結論 STEMI血小闆處于活化狀態,血小闆活化指標血小闆α顆粒糖蛋白可作為冠心病病情鑑測的有效指標,血小闆源生長因子受體αβ有參攷價值,掃描電鏡觀察可髮現血小闆活化的超微病理結構改變.
목적 탐토급성ST단태고심기경사(STEMI)환자혈소판α과립당단백(platelet activationdependent granule membrane protein,PADGEM or GMP-140,CD62P)、혈소판원생장인자수체αβ아형(platelet-derived growth factor receptor-αβ,PDGFR-αβ)표체,혈소판초미결구적변화.방법 채용류식세포의측정36례급성ST단태고심기경사환자화동기34명건강체검자(대조조)외주혈중혈소판적혈소판a과립당단백(CD62P)、혈소판원생장인자수체αβ아형(PDGFR-αβ)표체,채용소묘전자현미경관찰혈소판초미결구,병진행비교.결과 급성STEMI조치료전혈소판α과립당단백、혈소판원생장인자수체αβ표체솔분별위(3.65±1.87)%,(0.43±0.39)%,치료후위(0.96±0.79)%,(0.28±0.24)%,대조조상응위(0.67±0.35)%,(0.27±0.22)%.STEMI조적혈소판α과립당단백、혈소판원생장인자수체αβ표체솔,치료전교대조조고(분별위P＜0.01화P＜0.05),치료후명현강저.STEMI조소묘전경관찰혈소판표이초미결구변화명현.결론 STEMI혈소판처우활화상태,혈소판활화지표혈소판α과립당단백가작위관심병병정감측적유효지표,혈소판원생장인자수체αβ유삼고개치,소묘전경관찰가발현혈소판활화적초미병리결구개변.
Objective To investigate the expressions of platelet activation-dependent granule membrane protein and platelet-derived growth factor receptor-αB, and the ultra-microstructure changes of platelets in patients with acute ST-segment elevation myocardial infarction(STEMI). Method The expressions of platelet activationdependent granule of glycoprotein (CD62P)and platelet derived growth factor receptor αβ subtype (PDGFR-αβ)of platelets in peripheral blood in 36 patients with acute ST-segment elevation myocardial infarction(STEMI) hospitalized and another 34 healthy subjects over the same period (control group) were investigated by flow cytometry and data were analyzed. The changes of ultra microstructure and activity of blood platelets in those patients and control group were observed under the scanning electron microscope. Results The expressions of CD62P and PDGFR-αβin patients with STEMI group before treatment were (3.65 ± 1.87) % and (0.43 ± 0.39) %, respectively, and those after treatment were (0.96 ± 0.79) % and (0.28 ± 0. 24) %, respectively, whereas those in control group were (0.67 ± 0.35) % and (0.27 ± 0.22) %, respectively, which were much lower in control than those in patients with STEMI before treatment (P ＜ 0.01 or P ＜ 0.05) respectively. There were statistically significant differences in the expressions of CD62P and PDGFR-αβ in patients group between pre-treatment and posttreatment (P ＜0.01 or P ＜0.05), respectively. Obvious ultra-microstructure changes of platelet surface in patients with STEMI group were observed. Conclusions Due to platelet activation in AMI, the expressions of CD62P can be used as effective indicators for monitoring coronary heart disease, and the PDGFR-αβ can be used as a reference indicator. The platelet surface ultra-microstructure changes during platelet activation in patients with AMI can be found by scanning electron microscopy.
