CAJ | 학술논문

目的观察丹参-川芎水提取物有效组分配伍的注射液对大鼠心肌缺血/再灌注(I/R)损伤的影响.方法将SD大鼠按随机数字表法分为假手术组、模型组、冠心宁组及丹参-川芎低、高剂量组,每组10只.采用结扎冠状动脉左前降支建立I/R损伤模型.于结扎成功后10 min股静脉注射给药,其中冠心宁组给予冠心宁注射液含生药2.88 g/kg,丹参-川芎低、高剂量组分别给予丹参-川芎水提取物有效组分注射液含生药2.43 g/kg和4.86 g/kg;假手术组和模型组给予等量生理盐水.于缺血40 min、再灌注120 min取血后处死动物,测定血中肌钙蛋白T(cTnT)、肌酸激酶同工酶(CK-MB)、6-酮-前列腺素F_(1a)(6-keto-PGF_(1a))、血栓素B_2(TXB_2)含量和血小板最大聚集率,并且测定心肌梗死程度.结果丹参-川芎低、高剂量组心肌梗死面积((23.0±3.8)%、(20.8±4.7)%]较模型组[(29.1±3.2)%]显著降低(P＜0.05和P＜0.01);cTnT[(0.78±0.29)mg/L、(0.76±0.29)mg/L]和CK-MB[(891.5±252.5)U/L、(759.5±191.3)U/L]也较模型组[(1.04±0.14)mg/L,(1 268.2±256.5)U/L]显著降低(均P＜0.05),丹参-川芎高剂量组6-keto-PGF_(1a)较模型组升高[(206.7±35.6)ng/L比(138.6±28.9)ng/L,P＜0.05],血小板最大聚集率较模型组降低[(49.4±9.3)%比(77.1±16.7)%,P＜0.05].结论丹参-川芎水提取物有效组分配伍可减小心肌梗死面积,降低I/R后cTnT、CK-MB含量,提高6-keto-PGF_(1a)/TXB_2比值,从而减轻心肌I/R损伤.
목적 관찰단삼-천궁수제취물유효조분배오적주사액대대서심기결혈/재관주(I/R)손상적영향.방법 장SD대서안수궤수자표법분위가수술조、모형조、관심저조급단삼-천궁저、고제량조,매조10지.채용결찰관상동맥좌전강지건립I/R손상모형.우결찰성공후10 min고정맥주사급약,기중관심저조급여관심저주사액함생약2.88 g/kg,단삼-천궁저、고제량조분별급여단삼-천궁수제취물유효조분주사액함생약2.43 g/kg화4.86 g/kg;가수술조화모형조급여등량생리염수.우결혈40 min、재관주120 min취혈후처사동물,측정혈중기개단백T(cTnT)、기산격매동공매(CK-MB)、6-동-전렬선소F_(1a)(6-keto-PGF_(1a))、혈전소B_2(TXB_2)함량화혈소판최대취집솔,병차측정심기경사정도.결과 단삼-천궁저、고제량조심기경사면적((23.0±3.8)%、(20.8±4.7)%]교모형조[(29.1±3.2)%]현저강저(P＜0.05화P＜0.01);cTnT[(0.78±0.29)mg/L、(0.76±0.29)mg/L]화CK-MB[(891.5±252.5)U/L、(759.5±191.3)U/L]야교모형조[(1.04±0.14)mg/L,(1 268.2±256.5)U/L]현저강저(균P＜0.05),단삼-천궁고제량조6-keto-PGF_(1a)교모형조승고[(206.7±35.6)ng/L비(138.6±28.9)ng/L,P＜0.05],혈소판최대취집솔교모형조강저[(49.4±9.3)%비(77.1±16.7)%,P＜0.05].결론 단삼-천궁수제취물유효조분배오가감소심기경사면적,강저I/R후cTnT、CK-MB함량,제고6-keto-PGF_(1a)/TXB_2비치,종이감경심기I/R손상.
Objective To observe effects of effective components compatibility of aqueous extracts of Salviae Miltiorrhizae (丹参) and Rhizoma Chuanxiong (川芎) on myocardial ischemia/reperfusion (I/R)injury in rat.Methods Sprague-Dawley (SD) rats were randomly divided into sham group，model group,Guanxinning injection (冠心宁注射液) group and effective components of aqueous extracts from Salviae Miltiorrhizae and Rhizoma Chuanxiong salvianolic low dose group and high dose group,with 10 rats in each group.The myocardial I/R injury model was reproduced by ligation of the left anterior descending artery,and the experimental drugs were injected intravenously via femoral vein at 10 minutes after ligation.2.88 g/kg Guanxinning injection was given in Guanxinning group,and 2.43 g/kg or 4.86 g/kg effective components of aqueous extracts of Salviae Miltiorrhizae and Rhizoma Chuanxiong salvianolic was given in low dose group and high dose group,respectively,and equal volume of normal saline was given in sham group and model group.The anesthetic rats were sacrificed 40 minutes after isehemia and 120 minutes of reperfusion.Blood samples were collected before rats were sacrificed.The contents of serum troponin T (cTnT) and MB isoenzyme of creatine kinase (CK-MB) were determined,6-keto-prostaglandin F_(1a)(6-keto-PGF_(1a)),thromboxane B_2 (TXB_2) and platelet aggregation rate in blood plasma were assessed,and the degree of myocardial infarction in rats was determined.Results The myocardial infarction size in combined Salviae Miltiorrhizae and Rhizoma Chuanxiong low close group and high dose group[(23.0±3.8) %,(20.8±4.7)%]were lower significantly than that in model group[(29.1±3.2)%,P＜0.05 and P＜0.01],the contents of serum cTnT[(0.78±0.29) mg/L,(0.76±0.29) mg/L]and CK-MB[(891.5±252.5) U/L,(759.5±191.3) U/L]were lower significantly than those in model group[(1.04±0.14) mg/L,(1 268.2±256.5) U/L,all P＜0.05].The level of 6-keto-PGF_(1a) was higher significantly in high dose of the combination group than that in model group[(206.7±35.6) ng/L vs.(138.6±28.9) ng/L,P＜0.05],and platelet aggregation rate was inhibited significantly[(49.4±9.3)% vs.(77.1±16.7)%,P ＜ 0.05].Conclusion Effective components compatibility of aqueous extracts from Salviae Miltiorrhizae and Rhizoma Chuanxiong may reduce significantly the size of myocardial infarct and blood content of myocardial enzyme CK-MB and cTnT,and increase the ratio of 6-keto-PGF_(1a)/TXB_2,thus reducing myocardial I/R injury.