CAJ | 학술논문

选取2,3,6-三甲基-β-环糊精(TM-β-CD)作为流动相手性添加剂,建立了采用反相高效液相色谱法分离酸奶及含乳饮料中乳酸异构体的方法.实验采用hypersil ODS2-C18(250×5.0 mm,5 μm)色谱柱,以0.5 mmol/L pH 2.5的TM-β-CD(含4.5 mmol/L H2SO4)作为流动相,流速为1.0 mL/min,紫外检测波长为210 nm.实验考察了不同色谱柱、柱平衡时间、手性流动相添加剂浓度及pH值对分离效果的影响,并进一步研究了方法的线性范围、检出限、精密度及回收率.
선취2,3,6-삼갑기-β-배호정(TM-β-CD)작위류동상수성첨가제,건립료채용반상고효액상색보법분리산내급함유음료중유산이구체적방법.실험채용hypersil ODS2-C18(250×5.0 mm,5 μm)색보주,이0.5 mmol/L pH 2.5적TM-β-CD(함4.5 mmol/L H2SO4)작위류동상,류속위1.0 mL/min,자외검측파장위210 nm.실험고찰료불동색보주、주평형시간、수성류동상첨가제농도급pH치대분리효과적영향,병진일보연구료방법적선성범위、검출한、정밀도급회수솔.
A reversed phase-high performance liquid chromatographic (RP-HPLC) method for enantiomeric separation of lactic acid in yoghourt and milk beverage was developed using TM-β-CD as chiral mobile phase additives. The separation was performed on Hypersil ODS2-C18 column (250×5.0 mm,5 μm) with mobile phase of 0.5 mmol/L TM-β-CD solution containing 4.5 mmol/L H2SO4 (pH 2.5) at flow rate of 1.0 mL/min. The DAD detection wavelength was set at 210 nm. The effects of different columns, column equilibration time, pH and concentration of chiral mobile phase additives on separation of D, L-lactic acid were investigated, and the analytical performance of the developed method including linear range, detection limit and precision was also studied.