CAJ | 학술논문

以交联法制备壳聚糖纳米颗粒,用透射电子显微镜和Zeta电位仪对壳聚糖纳米颗粒进行表征,发现颗粒呈球形,粒径约为50 nm,微球表面光滑、球形圆整、颗粒比较均匀、分散性好,电位约为11.1 mv;琼脂糖凝胶电泳结果显示,壳聚糖纳米颗粒能有效地结合质粒DNA,并能保护所结合的DNA防止DNase I 的酶切;将含GFP基因的壳聚糖纳米颗粒复合物使用基因枪转化洋葱表皮细胞,在倒置荧光显微镜下观察,发现细胞表达了绿色荧光蛋白,表达效率为8%.
이교련법제비각취당납미과립,용투사전자현미경화Zeta전위의대각취당납미과립진행표정,발현과립정구형,립경약위50 nm,미구표면광활、구형원정、과립비교균균、분산성호,전위약위11.1 mv;경지당응효전영결과현시,각취당납미과립능유효지결합질립DNA,병능보호소결합적DNA방지DNase I 적매절;장함GFP기인적각취당납미과립복합물사용기인창전화양총표피세포,재도치형광현미경하관찰,발현세포표체료록색형광단백,표체효솔위8%.
Chitosan nanoparticles were synthesized with cocervation process.The particles were characterized with transmission electron microscope and Zeta-Sizer measurement.Chitosan nanoparticles were shown to be spherical structure distributed evenly, the average sizes of the particles were about 50 nm in diameter, and the particles were 11.1 mv charge.Plasmid DNA was absorbed to chitosan nanoparticles by electrostafic forces.Agarose gel electrophoresis showed that the pEGAD plasmid DNA was effectively absorbed to chitosan nanoparticles.Furthermore, the complexes could protect the absorbed DNA from DNase I damage.Chitosan nanoparticles-DNA mixed was transformed into the onion cell via particle bombardment, and onion cells were observed under invert-fluorescence-microscope.The results showed that transformed onions with GFP gene were obtained by particle bombardment successfully.Analysis showed that the reporter GFP gene was expressed with high efficiency (8 %).