中国组织工程研究与临床康复
中國組織工程研究與臨床康複
중국조직공정연구여림상강복
JOURNAL OF CLINICAL REHABILITATIVE TISSUE ENGINEERING RESEARCH
2011年
41期
7715-7718
,共4页
视网膜%胚胎%发育%促红细胞生成素%大鼠
視網膜%胚胎%髮育%促紅細胞生成素%大鼠
시망막%배태%발육%촉홍세포생성소%대서
背景:促红细胞生成素是低氧诱导因子1 的下游靶基因,受低氧诱导因子1 的调节.目的:观察大鼠视网膜胚胎期及出生后早期发育过程中促红细胞生成素的时空表达与视网膜发育的关系.方法:取胚胎12,16,20d 鼠胚及成年Wistar 大鼠各5 只,处死后摘除眼球,分离视网膜,用免疫组织化学方法、半定量反转录聚合酶联反应检测视网膜促红细胞生成素蛋白及促红细胞生成素 mRNA 的表达.结果与结论:胚胎期大鼠视网膜神经上皮及色素上皮细胞的胞浆及胞核内均有促红细胞生成素阳性表达,表达趋势随胎龄增长而逐渐减弱;成年鼠视网膜促红细胞生成素阳性表达最弱.说明大鼠视网膜胚胎发育过程中促红细胞生成素的表达存在时空上的变化,这种变化可能与大鼠视网膜胚胎期的发育密切相关.
揹景:促紅細胞生成素是低氧誘導因子1 的下遊靶基因,受低氧誘導因子1 的調節.目的:觀察大鼠視網膜胚胎期及齣生後早期髮育過程中促紅細胞生成素的時空錶達與視網膜髮育的關繫.方法:取胚胎12,16,20d 鼠胚及成年Wistar 大鼠各5 隻,處死後摘除眼毬,分離視網膜,用免疫組織化學方法、半定量反轉錄聚閤酶聯反應檢測視網膜促紅細胞生成素蛋白及促紅細胞生成素 mRNA 的錶達.結果與結論:胚胎期大鼠視網膜神經上皮及色素上皮細胞的胞漿及胞覈內均有促紅細胞生成素暘性錶達,錶達趨勢隨胎齡增長而逐漸減弱;成年鼠視網膜促紅細胞生成素暘性錶達最弱.說明大鼠視網膜胚胎髮育過程中促紅細胞生成素的錶達存在時空上的變化,這種變化可能與大鼠視網膜胚胎期的髮育密切相關.
배경:촉홍세포생성소시저양유도인자1 적하유파기인,수저양유도인자1 적조절.목적:관찰대서시망막배태기급출생후조기발육과정중촉홍세포생성소적시공표체여시망막발육적관계.방법:취배태12,16,20d 서배급성년Wistar 대서각5 지,처사후적제안구,분리시망막,용면역조직화학방법、반정량반전록취합매련반응검측시망막촉홍세포생성소단백급촉홍세포생성소 mRNA 적표체.결과여결론:배태기대서시망막신경상피급색소상피세포적포장급포핵내균유촉홍세포생성소양성표체,표체추세수태령증장이축점감약;성년서시망막촉홍세포생성소양성표체최약.설명대서시망막배태발육과정중촉홍세포생성소적표체존재시공상적변화,저충변화가능여대서시망막배태기적발육밀절상관.
BACKGROUND: Erythropoietin (EPO) is downstream target gene of hypoxia inducible factor-1 and regulated by hypoxia inducible factor-1.OBJECTIVE: To investigate EPO expression during embryonic development of rat retina and postnatal early development and explore the role of EPO in retinal development.METHODS: Five embryo of 12, 16, 20 days and five adult Wistar rats were sacrificed. Their eyeballs were harvested and retina was separated. EPO protein and mRNA expression was detected by immunohistochemistry and RT-PCR, respectively.
RESULTS AND CONCLUSION: EPO expression was detected in cytoplasm and nuclei of neuroepithelium of retina and pigment epithelial cells during embryonic development of rat retina. The expression was gradually decreased. EPO expression was the weakest in adult rat retina. Results showed that EPO expression exhibited spatial changes during embryonic development of rat retina, which may correlate closely to the embryonic development of rat retina.