CAJ | 학술논문

通过EMS诱变获得一个拟南芥突变体shrunken pollen grain1,spg1.根据横切片的观察结果,与野生型相比较,突变体观察到异常的花粉粒.遗传学的实验表明:spg1突变现象由一个隐性的单基因控制.通过图位克隆的方法,spg1突变位点定位在3号染色体的分子标记3 - AL138638 -6632和3- AL353865 -6814之间大约427kb的区间内.生物信息学的分析表明,在这个区间内没有任何已知的与育性相关的基因.以上结果说明SPG1是一个控制拟南芥雄性不育的新基因.
통과EMS유변획득일개의남개돌변체shrunken pollen grain1,spg1.근거횡절편적관찰결과,여야생형상비교,돌변체관찰도이상적화분립.유전학적실험표명:spg1돌변현상유일개은성적단기인공제.통과도위극륭적방법,spg1돌변위점정위재3호염색체적분자표기3 - AL138638 -6632화3- AL353865 -6814지간대약427kb적구간내.생물신식학적분석표명,재저개구간내몰유임하이지적여육성상관적기인.이상결과설명SPG1시일개공제의남개웅성불육적신기인.
An Arabidopsis male sterile mutantshrunken pollen grain1,spg1 was obtained through enthyl methane sulfona (EMS) mutagenesis strategy.By the observation of the transverse section,the mutant showed the aberrant microspores and pollen grains compared with wild type.Genetic assay indicated that spg1 phenotype was controlled by a single recessive mutant locus,and this locus was mapped to the region about 427kb flanked with genetic marker 3 - AL138638 - 6632 and 3 -AL353865 -6814 on chromosome 3 by map-based cloning approach.Bioinformatics analysis showed there were no known male sterile genes in this region.It indicated SPG1 was a new male sterile gene in Arabidopsis.