CAJ | 학술논문

[目的]为H-FABP基因运用于猪育种过程中的标记辅助选择提供基础资料。[方法]应用PCR-SSCP方法分析H-FABP基因在山西白猪、马身猪、大白猪、长白猪和杜洛克猪5个猪种的多态性,并研究基因型与肌内脂肪含量的相关性。[结果]在猪H-FABP基因内含子1扩增的片段上发现了一个多态性,检测到2个等位基因（A、B）,3种基因型（AA、AB、BB）,并对纯合子进行测序,发现SSCP的变异是由碱基C→T的替换造成的。基因型在不同猪种分布的多重比较表明,马身猪与山西白猪、长白猪、大白猪、杜洛克猪间基因型分布差异极显著（P〈0.01）,其他纯种猪群间基因型分布差异不显著（P〉0.05）。固定效应模型分析表明,肌内脂肪含量基因型间差异极显著（P〈0.01）。最小二乘分析表明,BB基因型个体与AA基因型个体比较肌内脂肪含量的差异显著（P〈0.05）。[结论]H-FABP基因对猪肉品质存在一定的影响。
[목적]위H-FABP기인운용우저육충과정중적표기보조선택제공기출자료。[방법]응용PCR-SSCP방법분석H-FABP기인재산서백저、마신저、대백저、장백저화두락극저5개저충적다태성,병연구기인형여기내지방함량적상관성。[결과]재저H-FABP기인내함자1확증적편단상발현료일개다태성,검측도2개등위기인（A、B）,3충기인형（AA、AB、BB）,병대순합자진행측서,발현SSCP적변이시유감기C→T적체환조성적。기인형재불동저충분포적다중비교표명,마신저여산서백저、장백저、대백저、두락극저간기인형분포차이겁현저（P〈0.01）,기타순충저군간기인형분포차이불현저（P〉0.05）。고정효응모형분석표명,기내지방함량기인형간차이겁현저（P〈0.01）。최소이승분석표명,BB기인형개체여AA기인형개체비교기내지방함량적차이현저（P〈0.05）。[결론]H-FABP기인대저육품질존재일정적영향。
[Objective] The aim was to provide basic reference for the use of H-FABP gene in marker-assisted selection of the breeding process of pig.[Method] Single-nucleotide polymorphisms of the H-FABP gene in Shanxi White pig,Mashen pig,Large White pig,Landrace and Duroc were tested by PCR-SSCP,and the correlation between genotype and intramuscular fat content in pigs were analyzed.[Result] One polymorphism was found in the amplified region of intron 1 of porcine H-FABP gene,in which two alleles（A and B）and three genotypes（AA,AB,and BB）were examined.C→T transition was detected by sequencing the homozygotes.The multiple comparison of the distribution of genotype in different pig varieties revealed that Mashen pig showed extremely significant difference（P0.01）in genotype distribution with Shanxi White,Landrace,Large White and Duroc breeds;whereas no significant differences（P0.05）were found in genotype distribution between other breeds.Based on the fixed effect model,extremely significant differences（P 0.01）were found in the intramuscular fat content among different H-FABP genotypes.Using least square analysis,it was found that there was significant differences（P 0.05）in the intramuscular fat content between the individuals of the BB genotypes and those of the AA genotypes.[Conclusion] The H-FABP genotype had significant effects on the meat quality.