CAJ | 학술논문

目的明确弥漫大B细胞淋巴瘤(DLBCL)患者常见的染色体改变及其与生存期的关系.方法采用改进的染色体制备方法,对30例经病理活组织检查确诊的DLBCL患者进行染色体分析,并予相应的化疗方案处理,统计各组患者的生存期.结果 28例获得满意的染色体分裂象,其中5例染色体核型正常,8例为多倍体,4例发生t( 14；18)(q32;q21),5例发生t(3；14)( q27；q32),2例发生t(2;3) (p11;q27),1例发生t(3;22) (q27;q11),2例涉及7号染色体与其他染色体间异位,1例涉及17号染色体与其他染色体间异位.染色体核型正常以及发生t( 14；18) (q32；q21)和3q+改变的患者,生存期分别为( 71.4±2.0)、(67.9±1.6)、(69.1±1.7)个月,较多倍体、7号染色体异常、17号染色体异常患者明显延长(均P＜0.05).Ⅰ+Ⅱ期患者中,正常染色体核型、t(14; 18)( q32；q21)以及3q+患者生存期较发生多倍体改变患者以及7号染色体发生改变患者明显延长(P＜0.05).Ⅲ+Ⅳ期患者中,发生多倍体改变患者生存期亦较发生t( 14; 18) (q32;q21)、3q+以及正常染色体核型患者缩短(P＜0.05).Ⅰ+Ⅱ期正常染色体核型、t( 14;18) (q32;q21)、3q+以及多倍体患者与Ⅲ+Ⅳ期生存期比较,差异均有统计学意义(均P＜ 0.05).结论依据恶性淋巴瘤患者染色体改变及其与恶性淋巴瘤分期等的相关性研究,可以预测淋巴瘤患者的治疗效果、生存期等.
목적 명학미만대B세포림파류(DLBCL)환자상견적염색체개변급기여생존기적관계.방법 채용개진적염색체제비방법,대30례경병리활조직검사학진적DLBCL환자진행염색체분석,병여상응적화료방안처리,통계각조환자적생존기.결과 28례획득만의적염색체분렬상,기중5례염색체핵형정상,8례위다배체,4례발생t( 14；18)(q32;q21),5례발생t(3；14)( q27；q32),2례발생t(2;3) (p11;q27),1례발생t(3;22) (q27;q11),2례섭급7호염색체여기타염색체간이위,1례섭급17호염색체여기타염색체간이위.염색체핵형정상이급발생t( 14；18) (q32；q21)화3q+개변적환자,생존기분별위( 71.4±2.0)、(67.9±1.6)、(69.1±1.7)개월,교다배체、7호염색체이상、17호염색체이상환자명현연장(균P＜0.05).Ⅰ+Ⅱ기환자중,정상염색체핵형、t(14; 18)( q32；q21)이급3q+환자생존기교발생다배체개변환자이급7호염색체발생개변환자명현연장(P＜0.05).Ⅲ+Ⅳ기환자중,발생다배체개변환자생존기역교발생t( 14; 18) (q32;q21)、3q+이급정상염색체핵형환자축단(P＜0.05).Ⅰ+Ⅱ기정상염색체핵형、t( 14;18) (q32;q21)、3q+이급다배체환자여Ⅲ+Ⅳ기생존기비교,차이균유통계학의의(균P＜ 0.05).결론 의거악성림파류환자염색체개변급기여악성림파류분기등적상관성연구,가이예측림파류환자적치료효과、생존기등.
Objective To study the relationship between chromosomal abnormalities of diffuse large B-cell lymphoma and its survival time.Methods Chromosome preparations were made by using modified method.Karyotypes were analyzed by stain of G-banding. And all patients were treated by chemotherapy. All patients' survival time was calculated.Results Mitotic cells that could be used for analysis were found in 28 cases.5 of 28 karyotypes were normal and 8 cases were polyploid.There were 4 cases with t(14,18)(q32;q21),5 cases with t(3; 14) (q27;q32),2 cases with t(2;3) (p11 ;q27),1 case with t(3 ;22) (q27 ;q11) respectively.There were 2 cases with ectopia between 7 chromosome and other chromosomes and 1 case with ectopia between 17 chromosome and other chromosomes.The survival time of patients with normal karyotype,t(14,18) (q32;q21)or 3q+ was longer than that of other groups.The survival time of group in Ⅰ, Ⅱ stages was longer than that in Ⅲ, Ⅳ stages. Conclusion The treatment, survival time and prognosis could be expected according to chromosomal abnormalities and its relevance to stages in diffuse large B-cell lymphoma.