中华胰腺病杂志
中華胰腺病雜誌
중화이선병잡지
CHINESE JOURNAL OF PANCREATOLOGY
2011年
5期
315-317
,共3页
高道键%满晓华%徐岷%张玉琦%高军%龚燕芳%吴红玉%金晶%许国铭%李兆申
高道鍵%滿曉華%徐岷%張玉琦%高軍%龔燕芳%吳紅玉%金晶%許國銘%李兆申
고도건%만효화%서민%장옥기%고군%공연방%오홍옥%금정%허국명%리조신
胰腺肿瘤%组蛋白脱乙酰基酶类%RNA干扰%细胞周期
胰腺腫瘤%組蛋白脫乙酰基酶類%RNA榦擾%細胞週期
이선종류%조단백탈을선기매류%RNA간우%세포주기
Pancreatic neoplasms%Histone deacetylases%RNA interference%Cell cycle
目的 探讨组蛋白脱乙酰基酶1( HDAC1)基因沉默对人胰腺癌PaTu8988细胞周期影响及可能机制.方法 常规培养胰腺癌PaTu8988细胞,分为对照组、阴性siRNA转染(c-siRNA)组及15、30 nmol/L HDAC1 siRNA转染组.采用脂质体2000转染细胞48 h后,应用蛋白质印迹法检测细胞HDAC1基因沉默效率及p21基因表达；流式细胞法检测细胞周期的变化.结果 与对照组比较,30 nmol/L HDAC1 siRNA组PaTu8988细胞的HDAC1蛋白表达明显下降,P21蛋白表达明显增加；G2/M 期细胞比例显著减少[(21.48±3.67)％比(28.28±2.94)％,P＜0.05],S期细胞比例显著增加[(50.20±6.85)％比(32.49±2.78)％,P＜0.05].结论 HDACl siRNA能特异、有效地抑制人胰腺癌PaTu8988细胞HDACl的表达,引起细胞S期阻滞,其机制可能与上调p21蛋白表达有关.
目的 探討組蛋白脫乙酰基酶1( HDAC1)基因沉默對人胰腺癌PaTu8988細胞週期影響及可能機製.方法 常規培養胰腺癌PaTu8988細胞,分為對照組、陰性siRNA轉染(c-siRNA)組及15、30 nmol/L HDAC1 siRNA轉染組.採用脂質體2000轉染細胞48 h後,應用蛋白質印跡法檢測細胞HDAC1基因沉默效率及p21基因錶達；流式細胞法檢測細胞週期的變化.結果 與對照組比較,30 nmol/L HDAC1 siRNA組PaTu8988細胞的HDAC1蛋白錶達明顯下降,P21蛋白錶達明顯增加；G2/M 期細胞比例顯著減少[(21.48±3.67)％比(28.28±2.94)％,P＜0.05],S期細胞比例顯著增加[(50.20±6.85)％比(32.49±2.78)％,P＜0.05].結論 HDACl siRNA能特異、有效地抑製人胰腺癌PaTu8988細胞HDACl的錶達,引起細胞S期阻滯,其機製可能與上調p21蛋白錶達有關.
목적 탐토조단백탈을선기매1( HDAC1)기인침묵대인이선암PaTu8988세포주기영향급가능궤제.방법 상규배양이선암PaTu8988세포,분위대조조、음성siRNA전염(c-siRNA)조급15、30 nmol/L HDAC1 siRNA전염조.채용지질체2000전염세포48 h후,응용단백질인적법검측세포HDAC1기인침묵효솔급p21기인표체；류식세포법검측세포주기적변화.결과 여대조조비교,30 nmol/L HDAC1 siRNA조PaTu8988세포적HDAC1단백표체명현하강,P21단백표체명현증가；G2/M 기세포비례현저감소[(21.48±3.67)％비(28.28±2.94)％,P＜0.05],S기세포비례현저증가[(50.20±6.85)％비(32.49±2.78)％,P＜0.05].결론 HDACl siRNA능특이、유효지억제인이선암PaTu8988세포HDACl적표체,인기세포S기조체,기궤제가능여상조p21단백표체유관.
Objective To investigate the effects of histone deacetylase 1 (HDAC1) gene silencing on cell cycle of pancreatic cancer cell line PaTu8988 and possible mechanism.Methods The PaTu8988 cells were routinely cultured and divided into control group,negative control siRNA group (c-siRNA),HDAC1 siRNA 15 nmol/L group and HDAC1 siRNA 30nmol/L group.After Liposomes 2000 transfection for 48 h,the Western blotting was used to detect the efficiency of HDAC1 gene silencing on protein levels and the expression of p21 protein.Cell cycle was evaluated by using flow cytometry.Results Compared with that of control group,the expression of HDAC1 protein in PaTu8988 cell of HDAC1 siRNA 30nmol/L group was significantly decreased,and the expression of p21 protein was significantly increased; the percentage of G2/M phase cells were significantly decreased[(21.48 ±3.67)％ vs.(28.28 ±2.94) ％,P＜0.05]; while the percentage of S phase cells were significantly increased[( 50.20 ± 6.85 ) ％ vs.( 32.49 ± 2.78 ) ％,P ＜ 0.05].Conclusions HDAC1 siRNA can efficiently and specifically inhibit the expression of HDAC1 in PaTu8988 cells,and can induce S phase cells arrest,which may be related with up-regulation of p21 protein expression.
