中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2008年
1期
21-25
,共5页
陈远萍%韩光红%晋长伟%史瑞新%侯建华
陳遠萍%韓光紅%晉長偉%史瑞新%侯建華
진원평%한광홍%진장위%사서신%후건화
斯伐他汀%正畸学,矫正%牙移动%骨形成蛋白2
斯伐他汀%正畸學,矯正%牙移動%骨形成蛋白2
사벌타정%정기학,교정%아이동%골형성단백2
Simvastatin%Orthodontics,corrective%Tooth movement%Bone morphogenetic protein-2
目的 明确全身给予辛伐他汀对大鼠牙齿移动后复发的影响,探讨辛伐他汀促进牙齿稳定的作用机制.方法 选用32只雄性Wistar大鼠,随机分成4组:对照组(0.9%NaCl)、低剂量组(2.5 mg·kg-1 辛伐他汀)、中剂量组(5.0 mg·kg-1 辛伐他汀)、高剂量组(10.0 mg·kg-1辛伐他汀),牵引其上颌第一磨牙向近中移动,持续21 d.实验组在加力装置去除前1 d开始,腹膜下注射辛伐他汀,阴性对照组注射生理盐水,每天1次,连续4周.分别在加力装置去除时及其后1、4周,测量上颌第一、第二磨牙间距离.取上颌第一磨牙及其牙周组织行组织学切片,HE染色及骨形成蛋白2(BMP-2)免疫组织化学染色,并进行图像分析和统计.结果 ①低、中、高剂量组大鼠牙齿移动复发距离均小于对照组(P＜0.05)、复发率明显低于对照组(P＜0.01),且剂量越小复发程度越小,低剂量组复发率最低(1、4周的复发率分别为26.81%、53.38%);②牙周组织中BMP-2表达量,低、中、高剂量组均高于对照组,差异有统计学意义(P＜0.001),低剂量组灰度积分增高最明显(P＜0.001);牙周组织中BMP-2表达,张力区略高于压力区,差异无统计学意义(P＞0.05).结论 在正畸牙齿移动后复发的过程中,辛伐他汀能有效抑制实验性牙移动后牙齿复发的程度,低剂量的辛伐他汀效果最明显.其作用可能是通过促进牙周组织中BMP-2蛋白的表达,加速成骨细胞活动,促进骨形成,促进移动后的牙齿稳定.
目的 明確全身給予辛伐他汀對大鼠牙齒移動後複髮的影響,探討辛伐他汀促進牙齒穩定的作用機製.方法 選用32隻雄性Wistar大鼠,隨機分成4組:對照組(0.9%NaCl)、低劑量組(2.5 mg·kg-1 辛伐他汀)、中劑量組(5.0 mg·kg-1 辛伐他汀)、高劑量組(10.0 mg·kg-1辛伐他汀),牽引其上頜第一磨牙嚮近中移動,持續21 d.實驗組在加力裝置去除前1 d開始,腹膜下註射辛伐他汀,陰性對照組註射生理鹽水,每天1次,連續4週.分彆在加力裝置去除時及其後1、4週,測量上頜第一、第二磨牙間距離.取上頜第一磨牙及其牙週組織行組織學切片,HE染色及骨形成蛋白2(BMP-2)免疫組織化學染色,併進行圖像分析和統計.結果 ①低、中、高劑量組大鼠牙齒移動複髮距離均小于對照組(P＜0.05)、複髮率明顯低于對照組(P＜0.01),且劑量越小複髮程度越小,低劑量組複髮率最低(1、4週的複髮率分彆為26.81%、53.38%);②牙週組織中BMP-2錶達量,低、中、高劑量組均高于對照組,差異有統計學意義(P＜0.001),低劑量組灰度積分增高最明顯(P＜0.001);牙週組織中BMP-2錶達,張力區略高于壓力區,差異無統計學意義(P＞0.05).結論 在正畸牙齒移動後複髮的過程中,辛伐他汀能有效抑製實驗性牙移動後牙齒複髮的程度,低劑量的辛伐他汀效果最明顯.其作用可能是通過促進牙週組織中BMP-2蛋白的錶達,加速成骨細胞活動,促進骨形成,促進移動後的牙齒穩定.
목적 명학전신급여신벌타정대대서아치이동후복발적영향,탐토신벌타정촉진아치은정적작용궤제.방법 선용32지웅성Wistar대서,수궤분성4조:대조조(0.9%NaCl)、저제량조(2.5 mg·kg-1 신벌타정)、중제량조(5.0 mg·kg-1 신벌타정)、고제량조(10.0 mg·kg-1신벌타정),견인기상합제일마아향근중이동,지속21 d.실험조재가력장치거제전1 d개시,복막하주사신벌타정,음성대조조주사생리염수,매천1차,련속4주.분별재가력장치거제시급기후1、4주,측량상합제일、제이마아간거리.취상합제일마아급기아주조직행조직학절편,HE염색급골형성단백2(BMP-2)면역조직화학염색,병진행도상분석화통계.결과 ①저、중、고제량조대서아치이동복발거리균소우대조조(P＜0.05)、복발솔명현저우대조조(P＜0.01),차제량월소복발정도월소,저제량조복발솔최저(1、4주적복발솔분별위26.81%、53.38%);②아주조직중BMP-2표체량,저、중、고제량조균고우대조조,차이유통계학의의(P＜0.001),저제량조회도적분증고최명현(P＜0.001);아주조직중BMP-2표체,장력구략고우압력구,차이무통계학의의(P＞0.05).결론 재정기아치이동후복발적과정중,신벌타정능유효억제실험성아이동후아치복발적정도,저제량적신벌타정효과최명현.기작용가능시통과촉진아주조직중BMP-2단백적표체,가속성골세포활동,촉진골형성,촉진이동후적아치은정.
Objective To investigate the effect of systematic administration of simvastatin on the bone morphogenetie protein-2(BMP-2)expression in the periodontal tissue after rat tooth movement and on the relapse of tooth movement. Methods Orthodontic tooth movement of upper first molar was performed in 32 rats with coil spring for 21 days. The 32 rats were randomly allocated into 4 groups:negative control The simvastatin started to be administered to the experimental groups 1 day before appliances were removed,and once a day there after for 4 weeks. The negative control group received the isotonic saline only. The interdental distance between the first and second maxillary molars were measured,when appliances were removed, and 1 week and 4 weeks after tha. After the rats were sacrificed,sections of first maxillary molar and periodontal tissue were studied by immunohistochemistry. Results The number and percentage of relapse was lower in the three experimental groups than in the negative control group(P＜0.05,P＜0.01).The lower dose was given,the less relapse there was,with the lowerest dose resulting in lowest percentage of relapse(26.81% and 53.38%). BMP-2 expression in experimental groups was higher than in the negative control group,with the lowerest dose group showing the highest expression(P＜0.001). The BMP-2 expression on the tension side was slishtly stronger than that on the compression side(P＞0.05).Conclusions Systemie administration of simvastatin could decrease the extent of relapse of the orthodonticmoved tooth in rat, and the lower-dose of simvastatin seemed more effective. The possible mechanism for this may be that simvastatin functions by increasing the expression of BMP-2 in tlle periodontal tissue,accelerating the ossteoblast activity and promoting bone formation.
