CAJ | 학술논문

目的研究5-硝基吲唑-3-甲酰亚氨基二乙酸的细胞毒性,以及对HeLa细胞的放射增敏作用.方法取指数生长期的HeLa细胞,接种于96孔板,加入不同浓度的药物作用后,用噻唑蓝比色法(MTT法)测定存活分数,并比较不同药物剂量加照射的实验组与单纯照射组的细胞存活分数.结果 5-硝基吲唑-3-甲酰亚氨基二乙酸对HeLa细胞的毒性较小.该药物对HeLa细胞有放射增敏作用,且乏氧处理的效果优于有氧处理:0、6、12、24、48、96μg/ml药物乏氧处理,存活率分别为0.91、0.87、0.84、0.81、0.76、0.60;48、96μg/ml药物有氧处理,存活率分别为0.85和0.73.结论 5-硝基吲唑-3-甲酰亚氨基二乙酸对HeLa细胞的毒性较小,且具有一定的乏氧放射增敏剂特征.
목적 연구5-초기신서-3-갑선아안기이을산적세포독성,이급대HeLa세포적방사증민작용.방법 취지수생장기적HeLa세포,접충우96공판,가입불동농도적약물작용후,용새서람비색법(MTT법)측정존활분수,병비교불동약물제량가조사적실험조여단순조사조적세포존활분수.결과 5-초기신서-3-갑선아안기이을산대HeLa세포적독성교소.해약물대HeLa세포유방사증민작용,차핍양처리적효과우우유양처리:0、6、12、24、48、96μg/ml약물핍양처리,존활솔분별위0.91、0.87、0.84、0.81、0.76、0.60;48、96μg/ml약물유양처리,존활솔분별위0.85화0.73.결론 5-초기신서-3-갑선아안기이을산대HeLa세포적독성교소,차구유일정적핍양방사증민제특정.
Objective To investigate the cytotoxicity and radiosensitization of 5-nitroindazole-3-formyliminodiacetic acid on HeLa cells.Methods HeLa cells in exponential growth phase were incubated in culture media with different doses and the survival rate was determined by MTT assay.The survival rate of cells receiving radiation combined with different doses of medicine was compared with that of the control.Results The cytotoxicity of S-nitroindazole-3-formyliminodiacetic acid on HeLa cells was very low.The drug had hypoxia radiosensitizing effect on HeLa cells.At doses of 0,6,12,24,48 and 96 μg/ml under hypoxia ,the survival rate were 0.91 ,0.87,0.84,0.81 ,0.76 and 0.60,respectively.At the dosage of 48 and 96 μg/ml,the survival rate were 0.85 and 0.73 under oxygenous).Conclusions 5-Nitroindazole-3-formyliminodiacetic acid has low cytotoxicity and rediosensitizing effect on HeLa cells.