CAJ | 학술논문

目的探讨塞来昔布联合放射对人鼻咽癌细胞株CNE-2Z凋亡的影响及其可能的机制.方法实验分4组进行,分别为对照组、药物组(25μmol/L塞来昔布)、照射组(8 Gy X线)和实验组(25 μmol/L塞来昔布+8 Gy X线照射).克隆形成实验检测塞来昔布对CNE-2Z细胞的放射增敏作用;流式细胞仪检测肿瘤细胞凋亡率;免疫细胞化学染色检测B淋巴细胞-2(Bcl-2)、凋亡活化基因(Bax)蛋白表达;Western blot技术检测Caspase-3蛋白表达.结果塞来昔布对人鼻咽癌细胞CNE-2Z有放射增敏作用,实验组中CNE-2Z细胞平均存活分数为0.50,细胞平均致死剂量为2.36,与对照组比较差异有统计学意义(P值均＜0.01);塞来昔布联合放射能够上调Bax的蛋白表达,对照组Box表达评分为1.221 ±0.116,实验组为2.758±0.256;同时抑制Bcl-2蛋白表达,对照组Bel-2表达评分为2.559±0.144,实验组为1.253±0.114,差异均有统计学意义(P值均＜0.05);并明显增加肿瘤细胞的凋亡率(F=7.63,P＜0.01),差异有统计学意义;Western blot结果显示实验组Caspase-3蛋白表达增强.结论 Cox-2抑制剂塞来昔布联合放射能诱导人鼻咽癌细胞株CNE-2Z凋亡,具有放射增敏作用.
목적 탐토새래석포연합방사대인비인암세포주CNE-2Z조망적영향급기가능적궤제.방법 실험분4조진행,분별위대조조、약물조(25μmol/L새래석포)、조사조(8 Gy X선)화실험조(25 μmol/L새래석포+8 Gy X선조사).극륭형성실험검측새래석포대CNE-2Z세포적방사증민작용;류식세포의검측종류세포조망솔;면역세포화학염색검측B림파세포-2(Bcl-2)、조망활화기인(Bax)단백표체;Western blot기술검측Caspase-3단백표체.결과 새래석포대인비인암세포CNE-2Z유방사증민작용,실험조중CNE-2Z세포평균존활분수위0.50,세포평균치사제량위2.36,여대조조비교차이유통계학의의(P치균＜0.01);새래석포연합방사능구상조Bax적단백표체,대조조Box표체평분위1.221 ±0.116,실험조위2.758±0.256;동시억제Bcl-2단백표체,대조조Bel-2표체평분위2.559±0.144,실험조위1.253±0.114,차이균유통계학의의(P치균＜0.05);병명현증가종류세포적조망솔(F=7.63,P＜0.01),차이유통계학의의;Western blot결과현시실험조Caspase-3단백표체증강.결론 Cox-2억제제새래석포연합방사능유도인비인암세포주CNE-2Z조망,구유방사증민작용.
Objective To investigate the effects of celecoxib combined with radiotherapy on apoptosis of CNE-2Z cell lines and the potential mechanisms.Methods Four groups were used,a control,celecoxib (25 μmol/L celecoxib) ,irradiation ( 8 Gy X ray) and celecoxib plus irradiation.The radiosensitising effect was detected by clone formation experiment.Flow cytometry was used to detect the apoptosis rate of cells.The expressions of Bcl-2 and Bax were assessed by immunocytochemistry.Western blot was used to examine the expression of Caspase-3.Results Celecoxib enhanced the radiosensitivity of CNE-2Z cells.In experimental group,the mean surviving fraction and the mean lethal dose of CNE-2Z cells were 0.50 and 2.36 respectively.Compared with the irradiated group,there was significant differences between the two groups (P ＜ 0.01 ).Celecoxib combined with radiotherapy up-regulation the expression of Bax.The score of the expression of Bax in the control group and the experimental group were 1.221 ±0.116 and 2.758 ±0.256 respectively.Celecoxib combined with radiotherapy could inhibit the expression of the protein of Bcl-2.The score of the expression of Bcl-2 in the control group and the experimental group were 2.559 ± 0.144 and 1.253 ± 0.114 respectively,with significant differences ( P ＜ 0.01 ).Celecoxib combined with radiotherapy cound increase the apoptosis rate of tumor cells with significant differences ( F =7.63,P ＜ 0.01 ).Western blot showed that the expression of Caspase-3 was strengthened.Conclusion Celecoxib combined with radiotherapy could induce apoptosis and enhance the radiosensitivity of human nasopharyngeal carcinoma CNE-2Z cell lines.