CAJ | 학술논문

目的分析2007-2009年云南省腮腺炎病毒(MuV)分离株的SH和HN遗传特征.方法采用反转录-聚合酶链反应从Vero细胞分离的病毒株基因组中扩增出SH基因和HN基因,测序后用Mega4.1软件分析其遗传特征.结果云南省分离14株MuV的SH基因其核苷酸和氨基酸同源性为98.3%～100.0%和96.5%～100.0%,与其他省份比较其同源性为92.6%～99.4%和87.7%～100.0%,其中Wsh1和Wsh2与其他F基因型差异较大;与疫苗株同源性为84.5%～85.1%和77.2%;与其他基因型同源性为83.4%～90.9%和70.1%～86.0%.6株MuV分离株的HN基因与核苷酸和氨基酸同源性分别为99.3%～99.5%和99.1%～99.7%;与中国分离株SP株的核苷酸和氨基酸同源性均为99.8%;与其他基因型同源性为94.7%～96.8%和95.5%～99.1%;与疫苗株的同源性为92.4%～93.2%和95.5%～96.4%.结论 2007-2009年云南省流行的MuV均为F基因型;其HN基因比SH基因保守.
목적 분석2007-2009년운남성시선염병독(MuV)분리주적SH화HN유전특정.방법 채용반전록-취합매련반응종Vero세포분리적병독주기인조중확증출SH기인화HN기인,측서후용Mega4.1연건분석기유전특정.결과 운남성분리14주MuV적SH기인기핵감산화안기산동원성위98.3%～100.0%화96.5%～100.0%,여기타성빈비교기동원성위92.6%～99.4%화87.7%～100.0%,기중Wsh1화Wsh2여기타F기인형차이교대;여역묘주동원성위84.5%～85.1%화77.2%;여기타기인형동원성위83.4%～90.9%화70.1%～86.0%.6주MuV분리주적HN기인여핵감산화안기산동원성분별위99.3%～99.5%화99.1%～99.7%;여중국분리주SP주적핵감산화안기산동원성균위99.8%;여기타기인형동원성위94.7%～96.8%화95.5%～99.1%;여역묘주적동원성위92.4%～93.2%화95.5%～96.4%.결론 2007-2009년운남성류행적MuV균위F기인형;기HN기인비SH기인보수.
Objective To analyze genetic characterization of the small hydrophobic and hemagglutinin-neuraminidase genes of mumps virus(MuV)isolated in Yunnan province,China from 2007 to 2009.Methods Fourteen MuV strains were isolated in Yunnan,China from 2007 to 2009.Using RT-PCR,the SH gene fragments contained 316 nucleotides in all strains and HN gene of six strains were sequenced.The sequences were aligned with other mumps virus sequences downloaded from GenBank using Mega 4.1 software.Results Fourteen isolated strains were closely related to other reference strains of F genotypes.In SH gene,the homology of nucleotide and amino acid among the fourteen isolated strains were 98.3%-100.0%and 96.5%-100.0%,respectively,and 92.6%%-99.4%and 87.7%-100.0% of homology when compared with that of strains isolated from other provinces in China,respectively.Wsh1 and Wsh2 strains had less homology when compared to other strains of F genotypes.The fourteen strains had homology of 84.5%-85.1%and 77.2%compared to vaccine strains on nucleotide and amino acid,respectively,and had homology of 83.4%-90.9% and 70.1%-86.0% compared to that of other genotypes.In HN gene,the homology of nucleotide and amino acid among the six isolated strains were 99.3%-99.5% and 99.1%-99.7%,respectively,and also 99.8% and 99.8% of homology respectively when compared to the SP strain in China.All the six strains had homology of 92.4%-93.2% and 95.5%-96.4% when compared to the vaecine strains on nucleotide and amino acid,respectively,and had homology of 94.7%-96.8% and 95.5%-99.1%compared to other genotypes.Conclusion Fourteen strains isolated in Yunnan from 2007 to 2009belonged to F genotype of MuV while the HN gene seemed more conservative than SH gene.