CAJ | 학술논문

目的探讨纳米化仿生诱导骨促进兔脊柱融合的可行性.方法成骨化兔脂肪基质细胞(ADSCs)接种在纳米级β磷酸三钙/壳聚糖/聚已内酯基质,构建纳米化仿生诱导骨;建立兔腰5～6横突间融合模型,A组纳米化仿生诱导骨,B组自体髂骨,C组脂肪基质细胞移植,D组空白支架,E组rhBMP-2注射.行影像学、组织学、免疫组化、新骨及诱导因子定量分析等检查.结果 A组融合能力最强,融合率100%、新骨面积比(87.32±1.68)%、BMP含量及力学指标(128±19)、(331.56±3.21)N、(526.78±3.19)Nmm均高于其他组,差异有统计学意义(P＜0.05);B、C组次之,E组新骨生长不明显,融合相对延缓.D组未融合.结论仿生诱导骨能强化骨再生,促进脊柱融合.
목적 탐토납미화방생유도골촉진토척주융합적가행성.방법 성골화토지방기질세포(ADSCs)접충재납미급β린산삼개/각취당/취이내지기질,구건납미화방생유도골;건립토요5～6횡돌간융합모형,A조납미화방생유도골,B조자체가골,C조지방기질세포이식,D조공백지가,E조rhBMP-2주사.행영상학、조직학、면역조화、신골급유도인자정량분석등검사.결과 A조융합능력최강,융합솔100%、신골면적비(87.32±1.68)%、BMP함량급역학지표(128±19)、(331.56±3.21)N、(526.78±3.19)Nmm균고우기타조,차이유통계학의의(P＜0.05);B、C조차지,E조신골생장불명현,융합상대연완.D조미융합.결론 방생유도골능강화골재생,촉진척주융합.
Objective To investigate the applied value of mimetically osteoinductive bone in promoting spinal fusion of rabbits. Methods With aid of biological gels compound and dynamic (oscillating pattern) seeding, three-dimensional construction of mimetically osteoinductive bone was made by implanting adipose-derived stromal cells (ADSCs) into rhBMP-2-mediated nβ-TCP/Cs/PCL matrix. Lumbar 5-6intertranverse process fusion was established in New Zealand rabbits. The rabbits in group A were transplanted by mimetically osteoinductive bone, those in group B by bone autograft, those in groups C and E implanted by ADSCs and rhBMP-2 respectively, and those in group D given nβ-TCP/Cs/PCL matrix as blank scaffold. Histology, immunohistochemistry, new bone ratio, BMP quantification, and palpation and biomechanical examinations were performed at different periods. Results Compared with other groups,group A displayed remarkable fusion capacity with statistically significant difference according to data of new bone ratio and BMP content and biomechanical testing [100% of fusion rate, ( 87. 32 ± 1.68 ) % of new bone ratio, ( 128 ± 19) of BMP content, (331.56 ±3.21 ) N, (526. 78 ±3. 19) Nmm,P ＜0. 05].Conclusion Enhanced osteogenic potential can be achieved by using mimetically osteoinductive bone and spinal fusion can be promoted with concord pattern of osteoinductive and osteoconductive effects.