中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
7期
1257-1258
,共2页
冯彦博%陈建军%唐慧斌%王朝南%周进锋%余华晨
馮彥博%陳建軍%唐慧斌%王朝南%週進鋒%餘華晨
풍언박%진건군%당혜빈%왕조남%주진봉%여화신
钛合金%MG63细胞%生物相容性
鈦閤金%MG63細胞%生物相容性
태합금%MG63세포%생물상용성
Titanium Alloy%MG63 cell%Biocompatibility
目的 观察经磨砂、酸蚀、碱热复合处理的钛合金(Ti)表面对成骨样细胞(MG63细胞)生物相容性的影响.方法 以2×104或1×10/孔将MG63细胞分别接种于A组(光滑纯Ti表面),B组(磨砂、酸蚀处理表面)和C组(磨砂、酸蚀、碱热复合处理表面).对3组试样的MG63细胞的贴壁率、增殖率、碱性磷酸酶(ALP)活性进行检测,用扫描电镜(SEM)对共培养后试样表面MG63细胞的形态进行观察.结果 MG63细胞在A、B、C组的增殖率[吸光度(A)值]分别为0.752±0.080、0.614±0.020、0.567±0.062,A组与B、C组比较,差异有统计学意义(P＜0.05)；A、B、C组的ALP活性分别为0.450±0.014、0.465±0.012、0.510±0.036,C组与A组比较,差异有统计学意义(P＜0.05)；C组的细胞贴壁率高于A、B组(P＜0.05)；SEM可见C组试样表面MG63细胞贴附、伸展状态好于A、B组.结论 经磨砂、酸蚀、碱热复合处理的Ti表面对MG63细胞有良好的生物相容性,具有良好的细胞生物活性.
目的 觀察經磨砂、痠蝕、堿熱複閤處理的鈦閤金(Ti)錶麵對成骨樣細胞(MG63細胞)生物相容性的影響.方法 以2×104或1×10/孔將MG63細胞分彆接種于A組(光滑純Ti錶麵),B組(磨砂、痠蝕處理錶麵)和C組(磨砂、痠蝕、堿熱複閤處理錶麵).對3組試樣的MG63細胞的貼壁率、增殖率、堿性燐痠酶(ALP)活性進行檢測,用掃描電鏡(SEM)對共培養後試樣錶麵MG63細胞的形態進行觀察.結果 MG63細胞在A、B、C組的增殖率[吸光度(A)值]分彆為0.752±0.080、0.614±0.020、0.567±0.062,A組與B、C組比較,差異有統計學意義(P＜0.05)；A、B、C組的ALP活性分彆為0.450±0.014、0.465±0.012、0.510±0.036,C組與A組比較,差異有統計學意義(P＜0.05)；C組的細胞貼壁率高于A、B組(P＜0.05)；SEM可見C組試樣錶麵MG63細胞貼附、伸展狀態好于A、B組.結論 經磨砂、痠蝕、堿熱複閤處理的Ti錶麵對MG63細胞有良好的生物相容性,具有良好的細胞生物活性.
목적 관찰경마사、산식、감열복합처리적태합금(Ti)표면대성골양세포(MG63세포)생물상용성적영향.방법 이2×104혹1×10/공장MG63세포분별접충우A조(광활순Ti표면),B조(마사、산식처리표면)화C조(마사、산식、감열복합처리표면).대3조시양적MG63세포적첩벽솔、증식솔、감성린산매(ALP)활성진행검측,용소묘전경(SEM)대공배양후시양표면MG63세포적형태진행관찰.결과 MG63세포재A、B、C조적증식솔[흡광도(A)치]분별위0.752±0.080、0.614±0.020、0.567±0.062,A조여B、C조비교,차이유통계학의의(P＜0.05)；A、B、C조적ALP활성분별위0.450±0.014、0.465±0.012、0.510±0.036,C조여A조비교,차이유통계학의의(P＜0.05)；C조적세포첩벽솔고우A、B조(P＜0.05)；SEM가견C조시양표면MG63세포첩부、신전상태호우A、B조.결론 경마사、산식、감열복합처리적Ti표면대MG63세포유량호적생물상용성,구유량호적세포생물활성.
Objective To study effects of titanium alloy (Ti) by composite treatment using sandblasting acid etehing ( SLA ) and alkali-heat ( AH ) on cyto-biocompatibility of MG63 cells,Methods Osteoblast-like MG63 cells were cultured at 2 × 104 or 1×105 cells per well on samples of group A ( pure Ti),group B (SLA Ti) and group C ( SLA-AH Ti).The outcomes of cell adherence rate,cell proliferation rate,alkaline phosphatase (ALP) special assay were evaluated and compared and cell morphology was observed by scanning election microscopy (SEM).Results In group A,group B and group C,the cell proliferation rate was 0.752 ±0.080,0.614 ±0.020 and 0.567 ±0.062,respectively ( P ＜0.05,group A vs group B or group C),the ALP special assay was 0.450 ±0.014,0.465 ±0.012 and 0.510 ±0.036,respectively ( P ＜0.05,group C vs group A).The cell adherence rate in group C showed higher than in the two other groups ( P ＜0.05 ),and the cells on composite treated Ti were attached well and spread more extensively.Conclusion The surface of composite treated Ti is biocompatible and better bioactive than that of pure Ti or SLA Ti.
