CAJ | 학술논문

目的探讨慢性肾衰竭(CRF)大鼠肠道通透性及损伤的变化.方法雄性SD大鼠20只分为CRF组(n=10)和对照组(n=10),采用5/6肾切除制备CRF大鼠模型,定期监测血生化至模型成功建立.术后第12周,大鼠禁食12 h后灌饲含有1 g乳果糖(L)和0.5 g甘露醇(M)的测试液4 ml,收集大鼠口服测试液后6 h内的全部尿液,采用高压液相色谱-示差法(HPLC-RID),检测尿液排泄率比值(L/M),评估大鼠肠黏膜通透性水平;HE染色观察各组大鼠小肠黏膜光镜下的形态(肠黏膜绒毛高度、肌层厚度、绒毛数量)并行组织学损伤评分.结果 CRF组大鼠尿L/M比值高于对照组(1.75±0.11比1.20±0.06,P＜0.01);其肠黏膜绒毛高度、肌层厚度显著高于对照组(P＜0.01),绒毛数量显著低于对照组(P＜0.01).CRF组大鼠肠道病理组织学评分显著高于对照组(1.00±0.71比0,P＜0.01).结论 CRF大鼠肠道通透性增加,并且伴有不同程度的肠道损伤.
목적 탐토만성신쇠갈(CRF)대서장도통투성급손상적변화.방법 웅성SD대서20지분위CRF조(n=10)화대조조(n=10),채용5/6신절제제비CRF대서모형,정기감측혈생화지모형성공건립.술후제12주,대서금식12 h후관사함유1 g유과당(L)화0.5 g감로순(M)적측시액4 ml,수집대서구복측시액후6 h내적전부뇨액,채용고압액상색보-시차법(HPLC-RID),검측뇨액배설솔비치(L/M),평고대서장점막통투성수평;HE염색관찰각조대서소장점막광경하적형태(장점막융모고도、기층후도、융모수량)병행조직학손상평분.결과 CRF조대서뇨L/M비치고우대조조(1.75±0.11비1.20±0.06,P＜0.01);기장점막융모고도、기층후도현저고우대조조(P＜0.01),융모수량현저저우대조조(P＜0.01).CRF조대서장도병리조직학평분현저고우대조조(1.00±0.71비0,P＜0.01).결론 CRF대서장도통투성증가,병차반유불동정도적장도손상.
Objective To observe the histopathologic injury of small intestine and intestinal permeability in chronic renal failure (CRF) rats. Methods Twenty male Sprague-Dawley rats were randomly assigned to CRF group (n=10) and control group (n=10). 5/6 nephrectomy was used to establish CRF rats, while sham operation for control. Blood biochemistry was regularly monitored until CRF model was successfully established. The model rats were fed with lactulose (L) and mannitol (M) through intragastric administration. Urine was collected after 6 hours, and the concentration of lactulose and mannitol in urine was measured using high pressure liquid chromatograph with refractive index detector (HPLC-RID), and the ratio of urinary excretion of L/M was calculated to evaluate intestinal permeability. Small intestinal mucosa were stained by hematoxylin-eosin (HE) and observed with light microscope (villus height, thickness of muscle layer and villus count), histological damage score was used to evaluate intestinal injury. Results The L/M ratio of CRF group was higher than that of control group (1.75±0.11 vs 1.20±0.06, P<0.01). The small intestinal mucosal villus height and thickness of muscle layer in CRF group were higher (P<0.01), and the number of villi was lower compared to control group (P<0.01). The score of histopathologic intestine damage of CRF group was higher than that of control group (1.00±0.71 vs 0, P<0.01). Conclusion The intestinal permeability of CRF rats is increased with varying degrees of intestinal damage.