CAJ | 학술논문

目的建立一种可早期、快速检测肾综合征出血热(HFRS)患者血清中抗汉坦病毒IgM抗体的胶体金试纸条方法.方法用胶体金标记纯化的汉坦病毒重组核蛋白(rNP),将羊抗人μ链抗体固定于硝酸纤维素膜上作为检测区,以鼠抗汉坦病毒抗体作为质控区,组装成免疫层析试纸条检测HFRS确诊患者以及健康人血清,并与ELISA对照,评价其特异度和敏感度.结果在试纸条加入待检血清后10～15 min内即可获得结果,经临床ELISA确诊的50份HFRS患者血清均呈阳性,健康人血清标本均为阴性.结论该胶体金免疫层析快检纸条与ELISA法的检测结果具有很高符合率,可用于HFRS患者的早期诊断,该法操作简便、反应迅速、无需特殊仪器设备,适于在基层医疗单位推广使用.
목적 건립일충가조기、쾌속검측신종합정출혈열(HFRS)환자혈청중항한탄병독IgM항체적효체금시지조방법.방법 용효체금표기순화적한탄병독중조핵단백(rNP),장양항인μ련항체고정우초산섬유소막상작위검측구,이서항한탄병독항체작위질공구,조장성면역층석시지조검측HFRS학진환자이급건강인혈청,병여ELISA대조,평개기특이도화민감도.결과 재시지조가입대검혈청후10～15 min내즉가획득결과,경림상ELISA학진적50빈HFRS환자혈청균정양성,건강인혈청표본균위음성.결론 해효체금면역층석쾌검지조여ELISA법적검측결과구유흔고부합솔,가용우HFRS환자적조기진단,해법조작간편、반응신속、무수특수의기설비,괄우재기층의료단위추엄사용.
Objective To establish an early,simple and rapid colloidal gold strip method for the detection of IgM against hantavirus nucleocapsid protein in patients with hemorrhagic fever with renal syndrome(HFRS).Methods Purified recombinant Hantavirus nucleoprotein(rNP)was labeled by colloidal gold particles and then sprayed and fixed on fiberglass membrane as the combination pad.Anti-Human IgM(μ-chain specific)antibody produced in goat was fixed in the detection area,and mouse antihantavirus antibody was fixed in the quality control area.Both of them were on nitrocellous membrane strip in tandem.Together with a specimen pad ahead.The conbination pad and the nitrocellous membrane were assembled into a test strip.The colloidal gold strip assay was compared with ELISA for evaluation of specificity and sensitivity.Results The colloidal gold strip tests showed positive in the serum samples from 50 cases of HFRS which was clinically diagnosed and then verified by ELISA within 10-15 minutes.Whereas 30 serum samples of healthy donors have tested negative.Conclusions Our new colloidal gold immunochromatographic test strip method was well concordant with the ELISA assay,but the former was more raoid and simole.It could be used primary medical services.