侵袭与转移相关因子在脉络膜黑色素瘤中表达的研究
침습여전이상관인자재맥락막흑색소류중표체적연구
Research of factors related to invasion and metastasis in choroidal melanoma
  • 万方数据
    中华眼科杂志 중화안과잡지
    Chinese Journal of Ophthalmology
    2011年 7期 638-642 ,共5页

    牟莹莹%赵桂秋%林锦镛%赵洁%林红%胡丽婷%徐强%王青%孙为荣

    모형형%조계추%림금용%조길%림홍%호려정%서강%왕청%손위영

    脉络膜肿瘤%黑色素瘤%肿瘤侵袭%肿瘤转移%免疫组织化学 脈絡膜腫瘤%黑色素瘤%腫瘤侵襲%腫瘤轉移%免疫組織化學
    맥락막종류%흑색소류%종류침습%종류전이%면역조직화학
    Choroid neoplasms%Melanoma%Neoplasm invasiveness%Neoplasm metastasis%Immunohistochemistry
    目的 探讨脉络膜黑色素瘤中侵袭与转移相关因子缝隙连接蛋白43(Cx43)、上皮型钙黏附分子(E-cadherin)、磷脂酰肌醇-3激酶(PI3K)及结缔组织生长因子(CTGF)的表达及意义.方法 实验研究.采用免疫组织化学法检测27例脉络膜黑色素瘤组织及15例色素痣组织中Cx43、E-cadherin、PI3K及CTGF的表达,并与临床病理资料做对照分析,采用x2检验比较各因子表达阳性率的差异.结果 Cx43、E-cadherin、PI3K及CTGF蛋白在脉络膜黑色素瘤中的表达阳性率分别为74.07%(20/27),44.40%(12/27),74.07%(20/27)和66.67%(18/27),在色素痣中的表达阳性率分别为33.33%(5/15),86.67%(13/15),33.33%(5/15)和20.00%(3/15),Cx43、PI3K及CTGF蛋白在脉络膜黑色素瘤中的表达较色素痣增加,差异有统计学意义(x2=5.060,P=0.024;x2=5.060,P=0.024;x2=6.637,P=0.010).E-cadherin蛋白在脉络膜黑色素瘤中的表达较色素痣降低,差异有统计学意义(x2=5.490,P=0.019).Cx43蛋白在不同组织学类型的脉络膜黑色素瘤中表达阳性率不同:梭形细胞型为40%(4/10),混合型88.89%(8/9),类上皮型为100%(8/8),差异有统计学意义(x2=9.874,P=0.007).PI3K蛋白在不同大小的脉络膜黑色素瘤中表达阳性率不同:小肿瘤为42.86%(3/7),中肿瘤为75%(9/12),大肿瘤为100%(8/8),差异有统计学意义(x2=6.357,P=0.042).Cx43、E-cadherin、PI3K及CTGF蛋白在未累及巩膜的脉络膜黑色素瘤中的表达阳性率分别为50.00%(6/12),83.33%(10/12),50.00%(6/12)和41.66%(5/12),在累及巩膜的脉络膜黑色素瘤中的表达阳性率分别为93.33%(14/15),40.00%(6/15),93.33%(14/15)和86.67%(13/15),Cx43、PI3K及CTGF蛋白在脉络膜黑色素瘤中的表达,累及巩膜组较未累及组增加,差异有统计学意义(x2=4.457,P=0.016;x2=4.457,P=0.016;x2=4.218,P=0.019).E-cadherin在脉络膜黑色素瘤中的表达,累及巩膜组较未累及组降低,差异有统计学意义(x2=3.546,P=0.028).结论 Cx43、PI3K、CTGF蛋白的高表达及E-cadherin蛋白的低表达与脉络膜黑色素瘤的浸润和转移可能有一定的关系.
    목적 탐토맥락막흑색소류중침습여전이상관인자봉극련접단백43(Cx43)、상피형개점부분자(E-cadherin)、린지선기순-3격매(PI3K)급결체조직생장인자(CTGF)적표체급의의.방법 실험연구.채용면역조직화학법검측27례맥락막흑색소류조직급15례색소지조직중Cx43、E-cadherin、PI3K급CTGF적표체,병여림상병리자료주대조분석,채용x2검험비교각인자표체양성솔적차이.결과 Cx43、E-cadherin、PI3K급CTGF단백재맥락막흑색소류중적표체양성솔분별위74.07%(20/27),44.40%(12/27),74.07%(20/27)화66.67%(18/27),재색소지중적표체양성솔분별위33.33%(5/15),86.67%(13/15),33.33%(5/15)화20.00%(3/15),Cx43、PI3K급CTGF단백재맥락막흑색소류중적표체교색소지증가,차이유통계학의의(x2=5.060,P=0.024;x2=5.060,P=0.024;x2=6.637,P=0.010).E-cadherin단백재맥락막흑색소류중적표체교색소지강저,차이유통계학의의(x2=5.490,P=0.019).Cx43단백재불동조직학류형적맥락막흑색소류중표체양성솔불동:사형세포형위40%(4/10),혼합형88.89%(8/9),류상피형위100%(8/8),차이유통계학의의(x2=9.874,P=0.007).PI3K단백재불동대소적맥락막흑색소류중표체양성솔불동:소종류위42.86%(3/7),중종류위75%(9/12),대종류위100%(8/8),차이유통계학의의(x2=6.357,P=0.042).Cx43、E-cadherin、PI3K급CTGF단백재미루급공막적맥락막흑색소류중적표체양성솔분별위50.00%(6/12),83.33%(10/12),50.00%(6/12)화41.66%(5/12),재루급공막적맥락막흑색소류중적표체양성솔분별위93.33%(14/15),40.00%(6/15),93.33%(14/15)화86.67%(13/15),Cx43、PI3K급CTGF단백재맥락막흑색소류중적표체,루급공막조교미루급조증가,차이유통계학의의(x2=4.457,P=0.016;x2=4.457,P=0.016;x2=4.218,P=0.019).E-cadherin재맥락막흑색소류중적표체,루급공막조교미루급조강저,차이유통계학의의(x2=3.546,P=0.028).결론 Cx43、PI3K、CTGF단백적고표체급E-cadherin단백적저표체여맥락막흑색소류적침윤화전이가능유일정적관계.
    Objective To investigate the expression of factors related to invasion and metastasis in choroidal melanoma and to determine their relationships with malignant features. Methods The expression of Connexin43 ( Cx43 ) , epithelial cadherin ( E-cadherin) , phosphatidylinositol 3-kinase (PI3K) and connective tissue growth factor ( CTGF ) in choroidal melanoma and nevi were detected by immunohistochemistry, and the correlation between these factors and clinicopathological features were analyzed. Results Positive rates of Cx43, E-cadherin , PI3K and CTGF were 74. 07% (20/27) , 44.4%(12/27), 74.07% (20/27) and 66.67% (18/27) in choroidal melanoma tissues, respectively; and 33.33% (5/15), 86.67% (13/15), 33.33% (5/15) and 20.00% (3/15) in the nevi tissues,respectively. There were significant differences in the expression of these markers between the two groups (x2 =5.060, P=0.024; x2 =5.490, P = 0.019; x2 =5.060, P=0.024; x2 =6.637, P=0.010). The expression rates of Cx43 protein were 40% (4/10) , 88. 89% (8/9) and 100% (8/8) in spindle, mixed and epithelioid cell type, respectively. The expression of these data was related to histological type ( x2 =9. 874,P =0. 007). The expression rates of PI3K protein were 42. 86% (3/7) , 75% (9/12) and 100%( 8/8 ) , in small, medium and large tumors, respectively, and their expression were co-related to the tumor size ( x2 = 6. 357,P = 0. 042 ). Positive rates of Cx43, E-cadherin, PI3K and CTGF were 50% (6/12 ) ,83.33% (10/12), 50% (6/12) and 41.66% (5/12), respectively, in choroidal melanoma tissues without sclera invasion and were 93. 33% ( 14/15 ) , 40% ( 6/15 ) , 93. 33% ( 14/15 ) and 86. 67%(13/15), respectively, in choroidal melanoma tissues with violation involved the sclera. There were significant differences of the expression of these markers between the two groups ( x2 =4. 457, P = 0. 016;X2 = 3. 546, P = 0. 028; x2 = 4. 457, P = 0. 016; x2 = 4. 218, P = 0. 019 ) . Conclusion Increased expression of Cx43, PI3K and CTGF and decreased expression of E-cadherin are involved in the processes of invasion and metastasis of choroidal melanoma.
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