CAJ | 학술논문

用膜片钳技术研究了Transwell小室趋化迁移后的鼻咽癌CNE-2Z细胞容积激活性Cl-电流.47%低渗刺激迁移后的CNE-2Z细胞诱发容积激活性氯电流,与未迁移细胞相比,其特性以及其对氯通道阻断剂的敏感性发生明显的变化,此电流的密度明显高于未迁移细胞,而且该电流几乎完全被氯通道阻断剂adenosine-5'-triphosphate(ATP,10 mmol/L)、5-nitro-2-3-phenylpropylamino benzoic acid(NPPB,100μmol/L)和他莫昔芬(30 μmol/L)抑制,其中NPPB和他莫昔芬对迁移细胞的抑制作用明显强于未迁移细胞.迁移后的CNE-2Z细胞容积激活性氯通道对阴离子的通透性为:Br-＞Cl-＞Y＞葡萄糖酸,与未迁移细胞(I＞Br-＞Cl-＞葡萄糖酸)不同.结果提示,容积激活性氯通道可能参与CNE-2Z细胞的迁移过程.
용막편겸기술연구료Transwell소실추화천이후적비인암CNE-2Z세포용적격활성Cl-전류.47%저삼자격천이후적CNE-2Z세포유발용적격활성록전류,여미천이세포상비,기특성이급기대록통도조단제적민감성발생명현적변화,차전류적밀도명현고우미천이세포,이차해전류궤호완전피록통도조단제adenosine-5'-triphosphate(ATP,10 mmol/L)、5-nitro-2-3-phenylpropylamino benzoic acid(NPPB,100μmol/L)화타막석분(30 μmol/L)억제,기중NPPB화타막석분대천이세포적억제작용명현강우미천이세포.천이후적CNE-2Z세포용적격활성록통도대음리자적통투성위:Br-＞Cl-＞Y＞포도당산,여미천이세포(I＞Br-＞Cl-＞포도당산)불동.결과제시,용적격활성록통도가능삼여CNE-2Z세포적천이과정.
The transwell chamber migration assay and the patch-clamp technique were used to investigate the volume-activated Cl- current (ICl.vol) in migrated nasopharyngeal carcinoma cells (CNE-2Z). 47％ hypotonic solution activated a ICl.vol in the migrated CNE-2Z cells.Compared with the control cells (non-migrated), the properties of this current and the sensitivity to Cl- channel blockers were changed.The current density in migrated CNE-2Z cells was higher than that in non-migrated cells. The current was almost completely inhibited by extracellular application of adenosine-5'-triphosphate (ATP, 10 mmol/L), 5-nitro-2-3-phenylpropylamino benzoic acid (NPPB, 100mmol/L) and tamoxifen (30 mmol/L) in all voltage steps applied. The inhibition of NPPB and tamoxifen on the current was stronger in migrated cells than that in non-migrated cells. The permeability sequence of the four anions was Br-＞Cl-＞I-＞Gluconate. The sequence was different from that of the non-migrated cells (I-＞ Br-＞Cl-＞Gluconate). The results suggest that volume-activated chloride channels may be involved in the CNE-2Z cell migration.