CAJ | 학술논문

利用改进的差异显示技术从烟草中分离受乙烯利调控的新基因.在分离到的24个差异片段中,通过点杂交验证,表明其中17个为阳性.对这些阳性片段的序列分析表明有4个新基因(DF6,DF8,DF9,DF13).其中DF8是一个未知基因,其他3个通过GenBank搜索可以找到一些同源的已知基因.RT-PCR分析表明,DF6、DF8、DF13在对照中也有表达,受乙烯利诱导其表达水平上调.而DF9则仅在处理植株中表达.
이용개진적차이현시기술종연초중분리수을희리조공적신기인.재분리도적24개차이편단중,통과점잡교험증,표명기중17개위양성.대저사양성편단적서렬분석표명유4개신기인(DF6,DF8,DF9,DF13).기중DF8시일개미지기인,기타3개통과GenBank수색가이조도일사동원적이지기인.RT-PCR분석표명,DF6、DF8、DF13재대조중야유표체,수을희리유도기표체수평상조.이DF9칙부재처리식주중표체.
An improved differential display approach was used to identify novel genes induced by the ethylene generator ethephon from tobacco leaves. Total 24 cDNA fragments that show different expression level between ethephon-treated and control plants were isolated. Dot blotting indicated that 17 of then were positive. Sequence analysis revealed that four of them ( DF6, DF8, DF9, DF13) represented novel genes. One of them (DF8) was an unknown gene, the other three were new cDNA fragments with homology with some known genes. RT-PCR analysis of the four novel cDNA clones revealed that three of them ( DF6, DF8, DF13) were expressed constitutively in untreated control plants, and had improved mRNA transcript level in ethephon treated tobacco plants. While DF9 mRNA occurred only in the treated plants.