CAJ | 학술논문

学术背景:骨髓间充质干细胞由于其易于获取,并可以在体外短期内大量扩增,是目前最有希望应用于软骨组织工程的干细胞.目的:对骨髓问充质干细胞在软骨缺损修复中的应用状况进行综述.检索策略: 由该论文的研究人员应用计算机检索1982-10/2006-12Pubmed数据库与骨髓间充质干细胞修复软骨缺损相关文献,检索词"Marrow;Mesenchymal stem cells:cartilage defect",限定语言种类为English,同时检索1982-10/2006-12中国科技成果数据库检索词"骨髓;间充质干细胞;软骨缺损",并限定文章语言种类为中文.共检索到126篇文献,对资料进行初审,纳入标准:①综述类及实验类文章;②中文核心期刊收录文献.排除标准:重复性研究.文献评价:文献的来源主要来自Pubmed数据库及中国科技成果数据库.文献类型为综述类及实验研究.资料综合:骨髓间充质干细胞在体内具有分化产生软骨的能力已被证明,而在体外培养条件下软骨表型的分化却是一个受多种因素限制的复杂过程,目前调控机制仍不明确.动物实验表明,骨髓间充质干细胞能够修复具有临床意义的骨和软骨缺损.虽然近年来对骨髓间充质干细胞及其在软骨组织工程方面的研究已取得较大进展,但对骨髓间充质干细胞分化各阶段细胞标志物、骨髓间充质干细胞的增殖、分化的控制及基因转染技术及临床应用的评估结果都有待进一步研究.结论:动物实验表明,骨髓间充质干细胞能够修复具有临床意义的骨和软骨缺损,虽然近年来对骨髓间充质干细胞及其在软骨组织工程方面的研究已取得较大进展,但对骨髓间充质干细胞的基础和临床研究中仍存在诸多问题需要解决.
학술배경:골수간충질간세포유우기역우획취,병가이재체외단기내대량확증,시목전최유희망응용우연골조직공정적간세포.목적:대골수문충질간세포재연골결손수복중적응용상황진행종술.검색책략: 유해논문적연구인원응용계산궤검색1982-10/2006-12Pubmed수거고여골수간충질간세포수복연골결손상관문헌,검색사"Marrow;Mesenchymal stem cells:cartilage defect",한정어언충류위English,동시검색1982-10/2006-12중국과기성과수거고검색사"골수;간충질간세포;연골결손",병한정문장어언충류위중문.공검색도126편문헌,대자료진행초심,납입표준:①종술류급실험류문장;②중문핵심기간수록문헌.배제표준:중복성연구.문헌평개:문헌적래원주요래자Pubmed수거고급중국과기성과수거고.문헌류형위종술류급실험연구.자료종합:골수간충질간세포재체내구유분화산생연골적능력이피증명,이재체외배양조건하연골표형적분화각시일개수다충인소한제적복잡과정,목전조공궤제잉불명학.동물실험표명,골수간충질간세포능구수복구유림상의의적골화연골결손.수연근년래대골수간충질간세포급기재연골조직공정방면적연구이취득교대진전,단대골수간충질간세포분화각계단세포표지물、골수간충질간세포적증식、분화적공제급기인전염기술급림상응용적평고결과도유대진일보연구.결론:동물실험표명,골수간충질간세포능구수복구유림상의의적골화연골결손,수연근년래대골수간충질간세포급기재연골조직공정방면적연구이취득교대진전,단대골수간충질간세포적기출화림상연구중잉존재제다문제수요해결.
BACKGROUND: Bone marrow mesenchymal stem cells are prospective used in cartilage tissue engineering due to easy acquire and plentiful amplification in vitro in a short term.OBJECTIVE: To summarize the application of bone marrow mesenchymal stem cells in repairing cartilage defect.RETRIEVAL STRATEGY: A computer-based online search was conducted in Pubmed for English language publications containing the key words of "marrow; mesenchymal stem cells; cartilage defect" from October 1982 to December 2006, Relevant data were also searched in China Scientific and Technological Achievement Database for Chinese language publications containing the key words of "marrow; mesenchymai stem cells; cartilage defect" from October 1982 to December 2006. There were 126Exclusion criteria: duplicated articles.LITERATURE EVALUATION: Literatures including reviews and experimental studies were mainly derived from Pubmed database and China Scientific and Technological Achievement Database.DATA SYNTHESIS: It has been proved that bone marrow mesenchymal stem cells could differentiate into cartilage in vivo.However, differentiation of cartilage phenotype in vitro was restricted by multiple factors, and the controlling mechanism is still unclear up to now. Animal experiments demonstrated that bone marrow mesenchymal stem cells could repair bone and cartilage defect. Although studies on bone marrow mesenchymal stem cells and cartilage tissue engineering have developed to a certain degree, clinical applications and evaluations, including cell marks of bone marrow mesenchymal stem cells in different differentiated stages, controls of proliferation and differentiation of bone marrow mesenchymal stem cells, and gene transfection technique, still need a further study.CONCLUSION: Animal experiments indicate that bone marrow mesenchymal stem cells can significantly repair bone and cartilage defect. Although studies on bone marrow mesenchymal stem cells and cartilage tissue engineering have developed to a certain degree, problems of bone marrow mesenchymal stem cells in basic and clinical researches still need to be solved further.