CAJ | 학술논문

采用喷雾法制备了载牛血清白蛋白(BSA)的海藻酸钙微球,通过比较微球的平均粒径和包埋率,优化了喷雾法的制备参数,使微球对BSA的包埋率大于60%、载药率达8%以上.释放液中BSA的SDS-聚丙烯酰胺凝胶电泳、Western免疫印迹以及圆二色光谱显示,该制备方法并没有对被包埋蛋白的相对分子质量、抗原性以及二级结构产生影响.微球中BSA的体外释放曲线表明该给药系统具有pH响应特性,尤其在模拟胃液中,BSA的释放速率最慢、释放量最低,可以保护蛋白类药物免受胃酸等的破坏作用,达到肠部实现控制释放.
채용분무법제비료재우혈청백단백(BSA)적해조산개미구,통과비교미구적평균립경화포매솔,우화료분무법적제비삼수,사미구대BSA적포매솔대우60%、재약솔체8%이상.석방액중BSA적SDS-취병희선알응효전영、Western면역인적이급원이색광보현시,해제비방법병몰유대피포매단백적상대분자질량、항원성이급이급결구산생영향.미구중BSA적체외석방곡선표명해급약계통구유pH향응특성,우기재모의위액중,BSA적석방속솔최만、석방량최저,가이보호단백류약물면수위산등적파배작용,체도장부실현공제석방.
The alginate microspheres containing bovine serum albumin (BSA), chosen as a model protein, was prepared by spraying. The conditions of preparation were optimized by comparing the mean diameter of particles and the encapsulation efficiency of BSA. The results indicate that the encapsulation efficiency of BSA and the BSA loading are high (60% and 8%, respectively). The process of encapsulation has no effect on relative molecular mass, the molecular structure and the antigenicity of BSA by SDS-polyacrylamide gel electrophoresis (SDS-PAGE), Western-blot and circular dichroism (CD) spectrum. The in vitro release profile shows a controlled release of encapsulated BSA owing to the excellent pH responsive properties of alginate microspheres. Particularly in simulated gastric fluid, the release ratio and release amount of encapsulated BSA are low. Therefore, alginate microspheres are useful in intestinal-specific protein drugs delivery by protecting them from the destruction of gastric environment.